Accumulation of heavy metals in gametophytes of the epilithic mosses

Polishchuk, A. I.; Antonyak, Halyna

doi:10.30970/sbi.1302.601

Cited by 2 publications

(3 citation statements)

References 19 publications

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“…According to the results of the study, the concentrations of the analysed metals in P. polyantha moss can be arranged in a descending order as follows: Fe> Mn> Zn> Pb> Cu> Ni> Cr> Co> Cd. The pattern of heavy metal deposition in gametophytes of P. polyantha generally corresponds to the distribution of metallic elements recorded in other moss species collected from the city of Lviv and from different locations in Europe (Marinova et al, 2010;Harmens et al, 2013;Qarri et al 2014;Polishchuk & Antonyak, 2019.…”

Section: Resultssupporting

confidence: 68%

“…Bryophytes can resist the toxicity of heavy metals and accumulate significant amounts of these substances in their cells. The metal-accumulating property is inherent to both terrestrial and aquatic bryophytes (Macedo-Miranda et al 2016;Polishchuk & Antonyak, 2019Lesiv et al, 2020;Maresca et al, 2022). The ability of mosses to tolerate high concentrations of metals and metalloids and to resist their toxicity is of considerable interest.…”

Section: Introductionmentioning

confidence: 99%

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Metal-accumulating capacity and antioxidant activity of Pylaisia polyantha (Hedw.) Schimp. moss in urban areas

Polishchuk,

Zhylishchych,

Antonyak

2023

Biol. Stud.

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Background. Bryophytes, including mosses, are widely used in biomonitoring of atmospheric pollution due to their ability to accumulate metals from atmospheric air. However, the effects of metal accumulation on metabolic processes in bryophyte cells have not been thoroughly studied. The aim of this work was to analyse the accumulation of heavy metals, indices of lipid peroxidation (LPO), and antioxidant status in gametophytes of the epiphytic moss Pylaisia polyantha (Hedw.) Schimp. collected from urban areas with different levels of technogenic load. Materials and Methods. The study was conducted in the city of Lviv (western part of Ukraine). Within the city, 15 sampling sites were selected and grouped as follows: the green zone (A) conditionally used as the control, zone influenced by transport activities (B) and the industrial zone (C). In gametophyte shoots of P. polyantha, concentrations of heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) were analysed using the method of atomic absorption spectrophotometry; the content of LPO products (lipid hydroperoxides, thiobarbituric acid reactive substances (TBARS)) and the activities of antioxidant system enzymes (superoxide dismutase, catalase, glutathione S-transferase) were analysed using standard methods. The results were processed using standard statistical methods. Results. Concentrations of heavy metals in P. polyantha gametophytes collected from the study area can be arranged in descending order as follows: Fe> Mn> Zn> Pb> Cu> Ni> Cr> Co> Cd. The content of individual metals in moss material varied depending on the sampling site. Moss shoots collected from zone B accumulated significantly higher levels of Cd, Co, Cu, Fe, Mn, Pb and Zn, and shoots from zone C had higher levels of Cr and Zn compared to those from the green zone. Moss gametophytes collected from sites in zone B had higher concentrations of LPO products and increased levels of antioxidant enzymes activity compared to the control. Conclusions. Elevated concentrations of heavy metals in moss material from sites exposed to traffic and industrial activities reflect atmospheric heavy metal pollution in urban areas. Intensive accumulation of heavy metals in gametophytes of P. polyantha that grows in the areas of transport activities is accompanied by increased lipid peroxidation processes and activation of enzymes of the antioxidant system in moss cells. Activation of antioxidant enzymes may play an important role in the adaptation of the moss P. polyantha to urban environments contaminated with heavy metals as a result of anthropogenic activities.

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Section: Resultssupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Metal-accumulating capacity and antioxidant activity of Pylaisia polyantha (Hedw.) Schimp. moss in urban areas

Polishchuk,

Zhylishchych,

Antonyak

2023

Biol. Stud.

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“…1, demonstrate the accumulative capacity of Didymodon rigidulus moss for four heavy metals, which are often found in high concentrations in the environmental components of industrial cities. It has been found that the content of Cr, Ni, Pb and Zn in the D. rigidulus gametophyte as a whole reaches values of the same order of magnitude as in other previously studied epilithic mosses, such as Schistidium apocarpum and Rhynchostegium murale [20]. Depending on the level of their accumu lation in D. rigidulus shoots, the analyzed metals can be arranged in the following order of decreasing concentration: Zn> Cr> Ni> Pb.…”

Section: Resultsmentioning

confidence: 67%

Accumulation of heavy metals and antioxidant defense system in the gametophyte of Didymodon rigidulus Hedw. in areas with high traffic loads

Polishchuk

Antonyak

2021

Biol. Stud.

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Background. Various modes of transport are among the main sources of environmental pollution with heavy metals and other pollutants. Bryophytes are known to accumulate heavy metals; however, metabolic changes in mosses under conditions of metal accumulation have not been extensively studied. The aim of this article was to analyze the accumulation of heavy metals, the process of lipid peroxidation (LPO) and the activity of enzymes of the antioxidant system in Didymodon rigidulus Hedw. moss collected in urban areas with an intense load created by road and railway transport. Materials and Methods. Gametophyte shoots were collected at three sampling sites in the city of Lviv (Ukraine). Site 1 was selected in a park zone, which was considered a control one; sites 2 and 3 were selected in areas with heavy road and rail traffic, respectively. Concentrations of chromium (Cr), nickel (Ni), lead (Pb), and zinc (Zn) in the moss material were determined by atomic absorption spectrophotometry. The levels of LPO products, namely lipid hydroperoxides and thiobarbituric acid reactive substances (TBARS), as well as superoxide dismutase (SOD) and catalase activities, were determined by standard methods. The results were processed using the methods of variation statistics. Results. Moss D. rigidulus growing in the park area (site 1) accumulated metals in the following order of decreasing concentration: Zn> Cr> Ni> Pb. Gametophyte shoots of D. rigidulus collected at site 2 accumulated higher levels of Pb, Zn and Ni (by 2.27, 1.78 and 1.45 times, respectively), and at site 3, higher levels of Pb and Zn (by 1.8 and 1.67 times, respectively) compared to gametophytes collected in the park zone. In the moss samples from these sites, no significant differences in the Cr content were found as compared to the control. Concentration of lipid hydroperoxides in the moss sampled at sites 2 and 3 was 4.26 and 3.75 times higher, respectively, compared to the control, and TBARS production was more intense in plant material from site 2 than from the control site. SOD and catalase activities were considerably increased in D. rigidulus moss from site 2 compared with those from the control area; however, the activity of both enzymes in the moss samples from site 3 did not significantly exceed the control levels. Conclusions. Both road and rail traffic loads contribute to the accumulation of heavy metals, especially Zn and Pb, in D. rigidulus moss growing in the surrounding areas. Under such conditions, LPO process is stimulated, which is more pronounced in moss growing in area with heavy road traffic. The increased activity of antioxidant enzymes (SOD and catalase) in moss growing in this area can play an important role in protecting bryophyte cells against metal-induced oxidative stress under conditions of intense metal accumulation.

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Accumulation of heavy metals in gametophytes of the epilithic mosses

Cited by 2 publications

References 19 publications

Metal-accumulating capacity and antioxidant activity of Pylaisia polyantha (Hedw.) Schimp. moss in urban areas

Metal-accumulating capacity and antioxidant activity of Pylaisia polyantha (Hedw.) Schimp. moss in urban areas

Accumulation of heavy metals and antioxidant defense system in the gametophyte of Didymodon rigidulus Hedw. in areas with high traffic loads

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