Accumulation of glycine betaine in transplastomic potato plants expressing choline oxidase confers improved drought tolerance

You, Lili; Song, Qiping; Wu, Yuyong; Li, Shengchun; Jiang, Chunmei; Chang, Ling; Yang, Xinghong; Jiang, Zhang

doi:10.1007/s00425-019-03132-3

Cited by 55 publications

(28 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Leaf total RNAs (10 μg) were separated in a 1.2% (w/v) agarose‐formaldehyde gel, transferred to a nylon membrane (Hybond‐N + ; Amersham, GE Healthcare) by capillary blotting, and fixed to the membrane by baking in 80°C for 3 h, and hybridized with specific probes labeled with 32 P (for primers used, see Table S2). The hybridizations were carried out overnight at 42°C or 65°C (You et al 2019).…”

Section: Methodsmentioning

confidence: 99%

Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3

Wang

Yang

Zhang

et al. 2020

JIPB

View full text Add to dashboard Cite

To gain a better understanding of the molecular mechanisms of photosystem I (PSI) biogenesis, we characterized the Arabidopsis thaliana photosystem I biogenesis factor 2 (pbf2) mutant, which lacks PSI complex. PBF2 encodes a P-class pentatricopeptide repeat (PPR) protein. In the pbf2 mutants, we observed a striking decrease in the transcript level of only one gene, the chloroplast gene ycf3, which is essential for PSI assembly. Further analysis of ycf3 transcripts showed that PBF2 is specifically required for the splicing of ycf3 intron 1. Computational prediction of binding sequences and electrophoretic mobility shift assays reveal that PBF2 specifically binds to a sequence in ycf3 intron 1. Moreover, we found that PBF2 interacted with two general factors for group II intron splicing CHLOROPLAST RNA SPLICING2-ASSOCIATED FACTOR1 (CAF1) and CAF2, and facilitated the association of these two factors with ycf3 intron 1. Our results suggest that PBF2 is specifically required for the splicing of ycf3 intron 1 through cooperating with CAF1 and CAF2. Our results also suggest that additional proteins are required to contribute to the specificity of CAFdependent group II intron splicing.

show abstract

Section: Methodsmentioning

confidence: 99%

Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3

Wang

Yang

Zhang

et al. 2020

JIPB

View full text Add to dashboard Cite

show abstract

“…For Southern blot analyses, samples of 5 µg total DNA were digested with the restriction enzymes AgeI and MluI for 12-16 h, separated by electrophoresis in 1% agarose gels, and transferred onto Hybond nylon membranes (GE Healthcare) by capillary blotting. A PCR product covering a portion of the psbZ coding region (You et al ., 2019) was used as a hybridization probe.…”

Section: Methodsmentioning

confidence: 99%

Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

33 Transplastomic potato plants expressing double-stranded RNA (dsRNA) targeted 34 against essential genes of the Colorado potato beetle (CPB) can be lethal to larvae by 35 triggering an RNA interference (RNAi) response. High accumulation levels of 36 dsRNAs in plastids are crucial to confer an efficient RNAi response in the insects. 37 However, whether length and sequence of the dsRNA determine the efficacy of RNAi 38 and/or influence the level of dsRNA accumulation in plastids is not known. Here we 39 compared the RNAi efficacy of different lengths of dsRNA targeted against the CPB 40 β -Actin gene (ACT) by feeding in vitro-synthesized dsRNAs to larvae. We show that, 41 while the 60 bp dsRNA induced only a relatively low RNAi response in CPB, 42 dsRNAs of 200 bp and longer caused high mortality and similar larval growth 43 retardation. When the dsRNAs were expressed from the plastid (chloroplast) genome 44 of potato plants, we found that their accumulation levels were correlated with length. 45 dsRNA accumulation levels were positively associated with the observed mortality, 46 suppression of larval growth and suppression of target gene expression. Importantly, 47 transplastomic potato plants expressing the 200 bp dsRNA were better protected from 48 CPB than plants expressing the 297 bp dsRNA, the best-performing line in our 49 previous study. Our results suggest that the length of dsRNAs is an important factor 50 that influences their accumulation levels in plastids and thus determines the strength 51 of the insecticidal RNAi effect. Our findings will aid the design of optimized dsRNA 52 expression constructs for plant protection by plastid-mediated RNAi. 53 54 Key words: RNA interference; Plastid engineering; Transplastomic plants; 55 double-stranded RNA; Pest control; Colorado potato beetle 56 57 58 59

show abstract

“…The third or fourth leaf from the bottom to the top was harvested from each plant at 0, 4, and 8 days under drought stress, immediately frozen in liquid nitrogen and then stored at − 80 °C for qRT-PCR, MDA content and antioxidant activities quantifications. Total chlorophyll contents of each plant were measured by the method described by You et al (2019). All experiment was repeated at least three times.…”

Section: Drought Tolerance Assaysmentioning

confidence: 99%

Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

Wang

Yang

et al. 2021

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Phosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

show abstract

Accumulation of glycine betaine in transplastomic potato plants expressing choline oxidase confers improved drought tolerance

Cited by 55 publications

References 74 publications

Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3

Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3

Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle

Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

Contact Info

Product

Resources

About