Accumulation of Glucosinolates in Broccoli

Miao, Heng; Wang, Jiansheng; Cai, Congxi; Chang, Jiaqi; Zhao, Yong; Wang, Qiaomei

doi:10.1007/978-3-319-25462-3_16

Cited by 11 publications

(7 citation statements)

References 207 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The level of GLs and their composition vary considerably not only between different Brassica crops, but also between different tissues within the same crop ( 48 ). More than 17 different GL have been identified in broccoli, classified as aliphatic (derived from the amino acids methionine, alanine, valine, leucine, or isoleucine), indole (derived from tryptophan), and aromatic GLs (derived from phenylalanine or tyrosine) ( 49 ).…”

Section: Gls Chemical Structure Metabolism and Regulation In mentioning

confidence: 99%

“…The biosynthetic pathway of GLs has been thoroughly described in previous reviews ( 20 , 39 , 49 ). Aliphatic GLs are biosynthesized following three key independent steps: (1) the side chain elongation, (2) the GLs core structure building, and (3) the amino acid side chain modification ( >Figure 4 ).…”

Section: Gls Chemical Structure Metabolism and Regulation In mentioning

confidence: 99%

“…Simultaneously, GSH is produced by the action of specific ligases (GSH1 and GSH2) from cysteine, glutamate, and glycine ( Figure 4 , central part) and ensures sulfur supply for GL biosynthesis, yielding GSH conjugates ( 50 , 51 ). Alkenylation, benzoylation, glycosylation, hydroxylation, methoxylation, oxygenation, and sulfonation may affect the elongated amino acid, increasing the chemical structure diversity of GLs ( 20 , 39 , 49 ). The methylthioalkyl GLs are S-oxygenated in a reaction catalyzed by flavin monooxygenases (FMOGS-OXs) to methylsulfinylalkyl GLs, such as 4-methylsulfinylbutyl-GL ( Figure 6 ).…”

Section: Gls Chemical Structure Metabolism and Regulation In mentioning

confidence: 99%

See 2 more Smart Citations

Pre- and Post-harvest Factors Affecting Glucosinolate Content in Broccoli

et al. 2020

View full text Add to dashboard Cite

Owing to several presumed health-promoting biological activities, increased attention is being given to natural plant chemicals, especially those frequently entering the human diet. Glucosinolates (GLs) are the main bioactive compounds found in broccoli (Brassica oleracea L. var. italica Plenck). Their regular dietary assumption has been correlated with reduced risk of various types of neoplasms (lung, colon, pancreatic, breast, bladder, and prostate cancers), some degenerative diseases, such as Alzheimer's, and decreased incidence of cardiovascular pathologies. GL's synthesis pathway and regulation mechanism have been elucidated mainly in Arabidopsis. However, nearly 56 putative genes have been identified as involved in the B. oleracea GL pathway. It is widely recognized that there are several pre-harvest (genotype, growing environment, cultural practices, ripening stage, etc.) and post-harvest (harvesting, post-harvest treatments, packaging, storage, etc.) factors that affect GL synthesis, profiles, and levels in broccoli. Understanding how these factors act and interact in driving GL accumulation in the edible parts is essential for developing new broccoli cultivars with improved health-promoting bioactivity. In this regard, any systematic and comprehensive review outlining the effects of pre-and post-harvest factors on the accumulation of GLs in broccoli is not yet available. Thus, the goal of this paper is to fill this gap by giving a synoptic overview of the most relevant and recent literature. The existence of substantial cultivar-to-cultivar variation in GL content in response to pre-harvest factors and post-harvest manipulations has been highlighted and discussed. The paper also stresses the need for adapting particular pre-and post-harvest procedures for each particular genotype in order to maintain nutritious, fresh-like quality throughout the broccoli value chain.

show abstract

Section: Gls Chemical Structure Metabolism and Regulation In mentioning

confidence: 99%

Section: Gls Chemical Structure Metabolism and Regulation In mentioning

confidence: 99%

Section: Gls Chemical Structure Metabolism and Regulation In mentioning

confidence: 99%

See 1 more Smart Citation

Pre- and Post-harvest Factors Affecting Glucosinolate Content in Broccoli

et al. 2020

View full text Add to dashboard Cite

show abstract

“…As natural chemicals that facilitate defense responses against different types of stresses (biotic and abiotic) in plant [ 7 ], GSs/GSHPs, have other diverse functions that have caused them to quickly gain in popularity as a subject of growing scientific interest. Plants utilize the GSs-myrosinase system, the “mustard oil bomb” [ 8 ] as a self-defense system against microbes and herbivores [ 9 ], and lowering of myrosin cell activity makes plants more susceptible to predators and insects like aphids [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Human, Animal and Plant Health Benefits of Glucosinolates and Strategies for Enhanced Bioactivity: A Systematic Review

et al. 2020

View full text Add to dashboard Cite

Glucosinolates (GSs) are common anionic plant secondary metabolites in the order Brassicales. Together with glucosinolate hydrolysis products (GSHPs), they have recently gained much attention due to their biological activities and mechanisms of action. We review herein the health benefits of GSs/GSHPs, approaches to improve the plant contents, their bioavailability and bioactivity. In this review, only literature published between 2010 and March 2020 was retrieved from various scientific databases. Findings indicate that these compounds (natural, pure, synthetic, and derivatives) play an important role in human/animal health (disease therapy and prevention), plant health (defense chemicals, biofumigants/biocides), and food industries (preservatives). Overall, much interest is focused on in vitro studies as anti-cancer and antimicrobial agents. GS/GSHP levels improvement in plants utilizes mostly biotic/abiotic stresses and short periods of phytohormone application. Their availability and bioactivity are directly proportional to their contents at the source, which is affected by methods of food preparation, processing, and extraction. This review concludes that, to a greater extent, there is a need to explore and improve GS-rich sources, which should be emphasized to obtain natural bioactive compounds/active ingredients that can be included among synthetic and commercial products for use in maintaining and promoting health. Furthermore, the development of advanced research on compounds pharmacokinetics, their molecular mode of action, genetics based on biosynthesis, their uses in promoting the health of living organisms is highlighted.

show abstract

“…However, broccoli has high respiration and transpiration rates, which makes it very perishable [10]. Lots of post-harvest handlings have been investigated to extend its shelf life [11]. Nevertheless, commercial freezing is one good method to maintain the quality of broccoli and to allow it to be available throughout the year [12].…”

Section: Introductionmentioning

confidence: 99%

Main Health-Promoting Compounds Response to Long-Term Freezer Storage and Different Thawing Methods in Frozen Broccoli Florets

Miao

Lin

Zeng

et al. 2019

Foods

View full text Add to dashboard Cite

The effects of long-term freezer storage and different defrosting methods on the retention of glucosinolates, vitamin C, and total phenols in frozen broccoli florets were investigated in the present study. Frozen broccoli florets were stored in a freezer at −20 °C for 165 days or subjected to defrosting by three different house-hold thawing methods (water, air, and refrigerator defrosting). Results showed that all glucosinolates were well preserved, while vitamin C and total phenols were reduced by less than 12% and 19% of the control, respectively, during the storage. Besides, refrigerator and air defrosting were better than water defrosting in glucosinolates retention, and refrigerator defrosting was the best in vitamin C preservation. No difference was observed in reserving phenolic compounds among the three methods. In conclusion, long-term freezer storage is an excellent way to preserve broccoli florets, and refrigerator defrosting is the best way to maintain the nutritional compounds in frozen broccoli florets.

show abstract

Accumulation of Glucosinolates in Broccoli

Cited by 11 publications

References 207 publications

Pre- and Post-harvest Factors Affecting Glucosinolate Content in Broccoli

Pre- and Post-harvest Factors Affecting Glucosinolate Content in Broccoli

Human, Animal and Plant Health Benefits of Glucosinolates and Strategies for Enhanced Bioactivity: A Systematic Review

Main Health-Promoting Compounds Response to Long-Term Freezer Storage and Different Thawing Methods in Frozen Broccoli Florets

Contact Info

Product

Resources

About