Accumulation of dsRNA in endosomes contributes to inefficient RNA interference in the fall armyworm, Spodoptera frugiperda

Yoon, June‐Sun; Gurusamy, Dhandapani; Palli, Subba Reddy

doi:10.1016/j.ibmb.2017.09.011

Cited by 101 publications

(88 citation statements)

References 36 publications

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“…This tissue‐dependent variability in RNAi has already been described in many lepidopteran species . The lack of dsRNA uptake into the cell or the intracellular accumulation of dsRNA in endosomes, preventing the cleavage into small interference RNA, is one of the limiting factors that have been advanced to explain the low sensitivity of some tissues to RNAi . Given that in D. melanogaster and mammals, evidence is accumulated that NR4A receptors are implicated in the regulation of locomotor activity through their effects on both carbohydrate metabolism and glycogen homeostasis in the muscle , it seems reasonable to assume that AiHR38 exerts a similar function in A. ipsilon males.…”

Section: Discussionmentioning

confidence: 93%

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

et al. 2018

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In the male moth, Agrotis ipsilon, the behavioural response and neuron sensitivity within the olfactory centres, the antennal lobes (ALs), to female sex pheromone increase with age, in correlation with the maturation of sex accessory glands (SAGs). By contrast, newly mated males cease to be attracted to sex pheromone and remate when their SAGs are refilled during the next night. The insect hormone receptor 38 (HR38), an ortholog of the vertebrate NR4A receptors, is a component of ecdysteroid signalling pathway which controls adult male physiology and behaviour. Here, we cloned the A. ipsilon HR38 (AiHR38) and explored its function in the coordination of reproductive events in the male. AiHR38 was detected in SAGs and ALs, and where its amount raised with age, in parallel with SAG protein content and sex pheromone responsiveness. By contrast, the AL and SAG AiHR38 expressions declined at 0-2 h after mating, in linking with depletion of SAG protein reserves and loss of sensitivity to sex pheromone. The increased AL and SAG AiHR38 expressions at 20-24 h postmating coincided with replenishing of SAGs and recovery of sensitivity to sex pheromone for a new mating. Moreover, AiHR38 knockdown resulted in reduction in SAG protein amount and disruption of sex pheromone-orientated flight. These results show that the insect HR38 is essential both for SAG activity, probably by controlling the protein synthesis, and display of male sexual behaviour, and that the concomitant regulation of its expression within SAGs and olfactory centres contributes to synchronisation between fertility and sexual activity. DATABASE: The nucleotide sequence of Agrotis ipsilon HR38 is available in the DDBJ/EMBL/GenBank databases under the accession number MF402845.

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Section: Discussionmentioning

confidence: 93%

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

et al. 2018

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“…This might be related to the differential response to externally administered dsRNA by insects, such as the degradation of dsRNA by ribonucleases in Lepidoptera . The dsRNAs cannot be processed into siRNA in lepidopteran cells because they are trapped in acidic bodies, which is one major factor contributing to inefficient RNAi response in lepidopteran insects . The knockdown of nuclease activity in the gut of Leptinotarsa decemlineata enhances RNAi efficiency, but this is not the case in Schistocerca gregaria …”

Section: Discussionmentioning

confidence: 99%

“…37,38 The dsRNAs cannot be processed into siRNA in lepidopteran cells because they are trapped in acidic bodies, which is one major factor contributing to inefficient RNAi response in lepidopteran insects. 39 The knockdown of nuclease activity in the gut of Leptinotarsa decemlineata enhances RNAi efficiency, but this is not the case in Schistocerca gregaria. 40 The interference effect of dsRNA transgenic lines on P. xylostella was not enhanced by a higher dsRNA expression level.…”

Section: Discussionmentioning

confidence: 99%

Silencing arginine kinase/integrin β₁subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella

Liu

Chen

et al. 2019

Pest Management Science

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Background Plutella xylostella is a devastating agricultural insect pest of cruciferous plants, including crops. Plant‐mediated RNA interference (RNAi) is currently being developed for plant protection. In this study, we investigated the response of P. xylostella exposed to transgenic Arabidopsis thaliana plants that expressed double‐stranded RNA (dsRNA) targeting P. xylostella genes of arginine kinase (PxAK) and integrin β1 subunit (Pxβ). RESULTS Transgenic plants producing dsRNAs of the 384‐bp fragment of PxAK (dsAK plants), the 497‐bp fragment of Pxβ (dsβ plants), and the 881 bp of the combination of both genes (dsAK‐β plants) were generated and verified. Insect bioassay with these transgenic plants showed that the development of P. xylostella was affected, causing longer developmental time, and lower pupal weight and pupation rate. P. xylostella mortality rates were 25.0% when exposed to dsAK plants, 22.5% with dsβ plants, and 30.0% with dsAK‐β plants, which were all higher than 7.5% for the wild‐type plant. PxAK and Pxβ in P. xylostella were suppressed by 26.6–79.7% at the transcription level by the transgenic plants. CONCLUSION These results suggest that plant‐mediated RNAi targeting single gene or both PxAK and Pxβ may have the potential to control P. xylostella. © 2019 Society of Chemical Industry

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“…However, the accumulated literature reveals that achieving an effective RNAi response in insects is challenging and its efficiency varies greatly among insect orders . Notably, RNAi tends to be effective in Coleopterans, but lepidopteran insects exhibit significantly lower sensitivity due to various factors that include the low rate of dsRNA uptake into epithelial cells and degradation of dsRNA in the digestive system …”

Section: Introductionmentioning

confidence: 99%

Enhancing RNAi by using concatemerized double‐stranded RNA

Chandra

Asokan

Manamohan

et al. 2018

Pest Management Science

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Accumulation of dsRNA in endosomes contributes to inefficient RNA interference in the fall armyworm, Spodoptera frugiperda

Cited by 101 publications

References 36 publications

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

Silencing arginine kinase/integrin β₁subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella

Enhancing RNAi by using concatemerized double‐stranded RNA

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Accumulation of dsRNA in endosomes contributes to inefficient RNA interference in the fall armyworm, Spodoptera frugiperda

Cited by 101 publications

References 36 publications

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

The insect HR38 nuclear receptor, a member of the NR4A subfamily, is a synchronizer of reproductive activity in a moth

Silencing arginine kinase/integrin β1subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella

Enhancing RNAi by using concatemerized double‐stranded RNA

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Silencing arginine kinase/integrin β₁subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella