Accumulation of dry substances in hydrolysate during the processing of barley malt sprouts with a celluloid enzyme complex

Abstract

The article presents the results of a study of the enzymatic hydrolysis of barley malt sprouts by cellulolytic enzymes. The influence of the process parameters (temperature, substrate concentration, concentration of the enzyme preparation, duration of the process) on the output of dry substances in the hydrolysate is shown. The results of the experiments were presented by a mathematical model of the process and graphically-in the form of surface projections reflecting the mutual influence of each possible pair of factors on the dry matter content in the hydrolysate. It is found that all the studied factors have an effect on the accumulation of dry substances in the hydrolysate. As a result of optimization the parameters of the enzymatic hydrolysis process, the following parameter values were obtained: temperature - 50 ˚С, duration of enzymatic hydrolysis-89 min, substrate concentration - 0.37 (hydromodule 1:10), concentration of the enzyme preparation - 0.05 %.