Accumulation of Dietary DDE and Dieldrin by Largemouth Bass, Micropterus salmoides floridanus

Muller, Jennifer K.; Johnson, Kevin G.; Sepúlveda, Marı́a S.; Borgert, Christopher J.; Gross, Timothy S.

doi:10.1007/s00128-004-0535-5

Cited by 13 publications

(13 citation statements)

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“…Feed was administered to each tank at 1% mean tank body weight per each tank as described by Muller et al [3]. Feed doses of 1, 7, 37, and 185 mg/kg DDE were predicted to result in final carcass concentrations of 0.1, 0.63, 3, and 17 mg/kg DDE, respectively, based on 30% accumulation over 30 d. Feed doses of 0.02, 0.1, 0.6, and 3 mg/kg dieldrin were predicted to result in final carcass concentrations of 0.0018, 0.009, 0.054, and 0.27 mg/kg dieldrin, respectively, based on 50% accumulation.…”

Section: Methodsmentioning

confidence: 99%

“…Pesticide doses were chosen to create a range of whole‐carcass concentrations encompassing those reported for wild largemouth bass in reclaimed agriculture areas along the Ocklawaha River Basin (FL, USA) such as the ECMA. Target carcass concentrations were calculated assuming that only 30% of the DDE and 50% of the dieldrin ingested would accumulate in body tissues [3]. Characterization of the effects of these two pesticides may assist in determining if they act alone, or as part of a more complex mixture, in impairing the reproductive success of largemouth bass found in waterways contaminated with OCPs.…”

Section: Introductionmentioning

confidence: 99%

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Influence of seasonality and exposure on the accumulation and reproductive effects of p,p′‐dichlorodiphenyldichloroethane and dieldrin in largemouth bass

Johnson

Muller²,

Price³

et al. 2007

Enviro Toxic and Chemistry

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Two studies investigated the accumulation and reproductive effects of p,p'-dichlorodiphenyldichloroethane (DDE) and dieldrin over 30 or 120 d of oral exposure in captive Florida, USA, largemouth bass (Micropterus salmoides floridanus). The 30-d exposures were conducted during the peak reproductive season, and the 120-d study was conducted to simulate exposure throughout the ovarian cycle. Whole body chemical residue concentrations were similar, regardless of exposure duration, for the medium and high feed concentrations of either chemical; however, the low-dose residue concentrations were much lower, yet similar to natural exposures. No clear dose-response relationships were identified between chemical dose and morphological (length, weight, hepatosomatic index) or reproductive endpoints (sex steroid concentration, gonadosomatic index, percentage of fry hatching). Reproductive parameters were variable within treatment groups, indicating that circulating sex steroids and percent hatch endpoints have high natural variability among fish of the same age and reproductive stage. However, in general there was a decrease in plasma estradiol and 11-ketotestosterone for female and male fish, respectively, that were exposed to dieldrin. Overall, results suggest that exposure throughout ovarian (follicular) development to either DDE or dieldrin alone does not result in the depressed endocrine status and poor reproductive success reported in highly organochlorine pesticide-contaminated environments in Central Florida, USA.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of seasonality and exposure on the accumulation and reproductive effects of p,p′‐dichlorodiphenyldichloroethane and dieldrin in largemouth bass

Johnson

Muller²,

Price³

et al. 2007

Enviro Toxic and Chemistry

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“…Liver and gonadal tissues were excised, immediately flash frozen in liquid nitrogen, and stored at −80 °C until RNA was isolated. Carcasses were extracted and analyzed by gas chromatographymass spectrometry (GC-MS) for measurement of p,p′-DDE and dieldrin concentrations, using the method reported in Gelsleichter et al (2005) and described in detail in Muller et al (2004). The analyses were performed in the Analytical Toxicology Core Laboratory (University of Florida).…”

Section: Animals and Sample Collectionmentioning

confidence: 99%

Dietary exposure of largemouth bass to OCPs changes expression of genes important for reproduction

García-Reyero

Barber

Gross³

et al. 2006

Aquatic Toxicology

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Dieldrin and p,p′-DDE are ubiquitous contaminants known to act as endocrine disruptors, causing impaired development and reproduction in fish and wildlife. In order to elucidate the mechanisms by which dieldrin and p,p′-DDE cause endocrine disruption in largemouth bass (Micropterus salmoides), fish were exposed subchronically through the diet to both contaminants. Following 120 days of exposure, p,p′-DDE decreased estradiol in females, but increased 11-ketotestosterone in both sexes. Dieldrin on the other hand, decreased estradiol and 11-ketotestosterone in both sexes. Both pesticides also altered steady state mRNA expression levels of a set of genes chosen to represent three possible mechanisms of endocrine disruption: (1) direct interaction with soluble sex steroid receptors, (2) biosynthesis of endogenous sex hormones, and (3) metabolism of endogenous hormones. p,p′-DDE acted as a weak estrogen, increasing the expression of vitellogenin and estrogen receptor α in the liver. p,p′-DDE also altered the expression of genes involved in the synthesis of endogenous hormones as well as their metabolism. Dieldrin, on the other hand, only altered expression of vitellogenin and not estrogen receptor α . Dieldrin also altered the expression of genes involved in hormone synthesis and metabolism, and it dramatically lowered plasma hormone levels. Both pesticides targeted expression of genes involved in all three modes of action, suggesting that they each have multiple modes of action.

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“…Estradiol and 11-ketotestosterone (11-KT) were determined in LMB plasma by radioimmunoassay using the method described by Muller et al (2004). Estradiol antibody was purchased from ICN Biomedical (Costa Mesa, CA) and 11-KT antibody was purchased from Helix Biotech (Richmond, BC, Canada).…”

Section: Steroid Analysismentioning

confidence: 99%

Exposure to p,p′-DDE or dieldrin during the reproductive season alters hepatic CYP expression in largemouth bass (Micropterus salmoides)

et al. 2007

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Largemouth bass (LMB) in Central Florida living on sites with high levels of organochlorine pesticides (OCPs) have exhibited poor reproductive success and altered steroid profiles. The mechanism underlying these changes is unknown, however changes in the rate of steroid metabolism could alter steroid homeostasis. Members of the CYP2 and CYP3A families play a significant role in the metabolism of many xenobiotics and endogenous compounds, including sex steroids. Therefore, the goal of this study was to identify members of the CYP2 and CYP3A families in LMB and characterize the effects of OCP exposure on their expression. Full-length clones of two CYP3A isoforms were obtained from LMB liver, CYP3A68 and 3A69, which exhibited significant sequence divergence. Full-length clones for CYP2N14 and CYP2P11 were also obtained from LMB liver. Steady-state mRNA levels of each of these CYPs increased in both sexes between early reproductive phase (December) and peak reproductive phase (March). Expression of CYP3A68 and CYP2P11 was sexually dimorphic during peak reproductive phase with 2-fold higher expression in females and males, respectively. Foodborne exposure to 46 ppm p,p′-DDE or 0.8 ppm dieldrin for 30 days did not have a significant effect on expression of CYPs. However, 4 months exposure to p,p′-DDE induced CYP3A68 and 3A69 expression in both sexes, while dieldrin produced weak induction of CYP3A68 and suppressed CYP3A69 expression in females, but had no effect on males. Neither p,p ′-DDE nor dieldrin significantly altered the expression of CYP2P11 or CYP2N14. This work demonstrates that there are significant changes in CYP expression that occur during LMB reproduction which can be modified by exposure to OCPs.

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Accumulation of Dietary DDE and Dieldrin by Largemouth Bass, Micropterus salmoides floridanus

Cited by 13 publications

References 0 publications

Influence of seasonality and exposure on the accumulation and reproductive effects of p,p′‐dichlorodiphenyldichloroethane and dieldrin in largemouth bass

Influence of seasonality and exposure on the accumulation and reproductive effects of p,p′‐dichlorodiphenyldichloroethane and dieldrin in largemouth bass

Dietary exposure of largemouth bass to OCPs changes expression of genes important for reproduction

Exposure to p,p′-DDE or dieldrin during the reproductive season alters hepatic CYP expression in largemouth bass (Micropterus salmoides)

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