Accumulation of BEL1-like transcripts in solanaceous species

Campbell, Brian A.

doi:10.31274/rtd-180813-16677

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…The tobacco leaves were cut directly, and the phloem sap collection was performed as previously described (Campbell et al ., 2008). Total RNA was extracted using TRIzol reagent, and reverse transcription was performed using PrimeScript RT Reagent Kit.…”

Section: Methodsmentioning

confidence: 99%

Armet from whitefly saliva acts as an effector to suppress plant defences by targeting tobacco cystatin

Wang

et al. 2022

New Phytologist

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Summary Arginine rich, mutated in early stage of tumours (Armet), is a well‐characterized bifunctional protein as an unfolded protein response component intracellularly and a neurotrophic factor extracellularly in mammals. Recently, a new role of Armet as an effector protein mediating insect–plant interactions has been reported; however, its molecular mechanisms underlying the regulation of plant defences remain unclear. We investigated the molecular mechanisms underlying whitefly‐secreted Armet‐mediated regulation of insect–plant interaction by agrobacterium‐mediated transient expression, RNA interference, electrical penetration graph, protein–protein interaction studies, virus‐induced gene silencing assay, phytohormone analysis and whitefly bioassays. Armet, secreted by Bemisia tabaci whitefly, is highly expressed in the primary salivary gland and is delivered into tobacco plants during feeding. Overexpression of the BtArmet gene in tobacco enhanced whitefly performance, while silencing the BtArmet gene in whitefly interrupted whitefly feeding and suppressed whitefly performance on tobacco plants. BtArmet was shown to interact with NtCYS6, a cystatin protein essential for tobacco anti‐whitefly resistance, and counteract the negative effects of NtCYS6 on whitefly. These results indicate that BtArmet is a salivary effector and acts to promote whitefly performance on tobacco plants through binding to the tobacco cystatin NtCYS6. Our findings provide novel insight into whitefly–plant interactions.

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Section: Methodsmentioning

confidence: 99%

Armet from whitefly saliva acts as an effector to suppress plant defences by targeting tobacco cystatin

Wang

et al. 2022

New Phytologist

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“…The gene message persists in the phloem, although it decreases in fibres, probably due to the onset of lignification. The BP-like mRNAs occur in stem phloem (Tioni et al, 2003;Douglas and Riggs, 2005;Du et al, 2009), but their roles are still undefined during PG; complications arise because in situ experiments can also reveal sap-transported KNOX I mRNAs (Campbell et al, 2008;Ham et al, 2009) for long-distance functions (Kim et al, 2001). However, the ARK2-overexpressing and silenced lines of aspen showed delayed and precocious phloem differentiation during SG, respectively (Du et al, 2009).…”

Section: Knope1 Exerts Its Function In Peach Stem Elongation and Lignmentioning

confidence: 99%

The peach (Prunus persica L. Batsch) genome harbours 10 KNOX genes, which are differentially expressed in stem development, and the class 1 KNOPE1 regulates elongation and lignification during primary growth

Testone

Condello

Verde

et al. 2012

Journal of Experimental Botany

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The KNOTTED-like (KNOX) genes encode homeodomain transcription factors and regulate several processes of plant organ development. The peach (Prunus persica L. Batsch) genome was found to contain 10 KNOX members (KNOPE genes); six of them were experimentally located on the Prunus reference map and the class 1 KNOPE1 was found to link to a quantitative trait locus (QTL) for the internode length in the peach×Ferganensis population. All the KNOPE genes were differentially transcribed in the internodes of growing shoots; the KNOPE1 mRNA abundance decreased progressively from primary (elongation) to secondary growth (radial expansion). During primary growth, the KNOPE1 mRNA was localized in the cortex and in the procambium/metaphloem zones, whereas it was undetected in incipient phloem and xylem fibres. KNOPE1 overexpression in the Arabidopsis bp4 loss-of-function background (35S:KNOPE1/bp genotype) restored the rachis length, suggesting, together with the QTL association, a role for KNOPE1 in peach shoot elongation. Several lignin biosynthesis genes were up-regulated in the bp4 internodes but repressed in the 35S:KNOPE1/bp lines similarly to the wild type. Moreover, the lignin deposition pattern of the 35S:KNOPE1/bp and the wild-type internodes were the same. The KNOPE1 protein was found to recognize in vitro one of the typical KNOX DNA-binding sites that recurred in peach and Arabidopsis lignin genes. KNOPE1 expression was inversely correlated with that of lignin genes and lignin deposition along the peach shoot stems and was down-regulated in lignifying vascular tissues. These data strongly support that KNOPE1 prevents cell lignification by repressing lignin genes during peach stem primary growth.

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Accumulation of BEL1-like transcripts in solanaceous species

Cited by 2 publications

References 25 publications

Armet from whitefly saliva acts as an effector to suppress plant defences by targeting tobacco cystatin

Armet from whitefly saliva acts as an effector to suppress plant defences by targeting tobacco cystatin

The peach (Prunus persica L. Batsch) genome harbours 10 KNOX genes, which are differentially expressed in stem development, and the class 1 KNOPE1 regulates elongation and lignification during primary growth

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