Accumulation of Arginine in Plants Afflicted with Iron-Deficiency Type Chlorosis

Holley, Robert W.; Cain, John C.

doi:10.1126/science.121.3136.172

Cited by 29 publications

(11 citation statements)

References 12 publications

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“…An ornithine accumulation following chlorosis has never been reported to our knowledge. Only the related compound arginine does accumulate under certain tvpes of chlorosis (VON EULER andBuRSTROM 1933, HOLLEY andCAIN 1955). This was also observed in our experiments with etiolated leaves and amino-triazol treated bean shoots.…”

Section: Discussionsupporting

confidence: 87%

The Accumulation of L-Ornithine in Halo-Blight Infected Bean Plants (Phaseolus vulgaris L.), Induced by the Toxin of the Pathogen Pseudomonas phaseolicola (Burkh.) Dowson

Rudolph

Stahmann

1966

J Phytopathol

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Section: Discussionsupporting

confidence: 87%

The Accumulation of L-Ornithine in Halo-Blight Infected Bean Plants (Phaseolus vulgaris L.), Induced by the Toxin of the Pathogen Pseudomonas phaseolicola (Burkh.) Dowson

Rudolph

Stahmann

1966

J Phytopathol

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“…The relative shortage of one or more minerals is well known to lead to increased accumulation of free amino acids (Holley and Cain, 1955;Rabe, 1990;Ericsson et al, 1993). Achituv and Bar-Akiva (1973) and Rabe and Lovatt (1986) showed that phosphorus deficiency and consequential growth retardation leads to a strong accumulation of arginine in Citrus sp.…”

Section: Discussionmentioning

confidence: 99%

The effects of ammonium on growth, accumulation of free amino acids and nutritional status of young phosphorus deficient Stratiotes aloides plants

et al. 1996

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Turions of Stratiotes aloides L. were grown at different ammonium levels under phosphorus deficient conditions in a staged gradient. After 13 weeks the plantlets appeared to be severely phosphorus deficient with the growth being seriously impaired. Nitrogen in the plants appeared to be predominantly present as soluble amino acids. This indicates that net protein synthesis was seriously disturbed. Asparagine was by far the dominant soluble amino acid in the plantlets. At the highest extemal ammonium levels, net growth was significantly retarded when compared with the other concentrations with free ammonium accumulating in the plants. Ammonium uptake under phosphorus deficient conditions strongly increases nutrient imbalances in the plants, and thus their vitality.

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“…Arginine and the arginine pathway intermediates, ornithine and citrulline accumulate in leaves during K, P, Mg, S, Fe, Mn, Cl, and Cu deficiencies in a variety of vascular plant species (1,5,6,8,10,12,15,19,21,23,24 …”

mentioning

confidence: 99%

Increased Arginine Biosynthesis during Phosphorus Deficiency

Rabe

Lovatt²

1986

Plant Physiol.

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ABSTRACIT he accumulation of arginine in leaves of four citrus rootstock cultivars during P deficiency has been demonstrated to be due to increased de novo synthesis rather than decreased catabolism or increased protein degradation (E Rabe, CJ Lovatt, 1984, Plant Physiol 76: 747-752). In this report, we provide evidence (a) that the increased activity of the arginine biosynthetic pathway observed for citrus rootstocks grown under Pdeficient conditions for 7 months is due to an increase in the concentration of ammonia in leaves of P-deficient plants and (b) that ammonia accumulation and removal through arginine systhesis are early responses to phosphorus deficiency for both a woody perenniaL rough lemon (Citrus limon), and an herbaceous annuaL summer squash (Cucurbita pepo). Transferring 5-day-old squash plants to a phosphorus-deficient nutrient solution for only 10 days resulted in a 2-fold increase in the concentration of nitrate in the youngest fully expanded leaves (YFE). Concomitantly, the specific activity of nitrate reductase doubled and the ammonia content of P-deficient YFE leaves increased to a concentration significantly greater that of leaves from healthy control plants (P < 0.05). Consistent with increased availability of ammonia, the incorporation of NaH'4CO3 into arginine plus urea doubled during phosphorus deficiency and arginine accunlulated. Despite the accumulation of nitrate and ammonia in YFE leaves during phosphorus deficiency, the total nitrogen content of these leaves was less than that of the healthy control plants. Similar results were obtained for rough lemon. Nitrate content of the YFE leaves increased 1.5-and 3.0-fold in plants deprived of phosphorus for 6 and 12 weeks, respectively. Ammonia content of the leaves increased as P deficiency progressed to 1.4 ± 0.08 mg (± sE, n = 4) per gram dry weight, a level 1.8-fold greater than that of the P-sufficient control plants. During P deficiency de novo arginine biosynthesis in rough lemon increased 10-fold. Immersing the petiole of YFE leaves from P-sufficient squash and rough lemon plants in 50 millimolar NH4' for 3 hours resulted in the accumulation of ammonia in the leaves, and a 4-fold increase in the incorporation of NaH"CO3 into arginine plus urea. Taken together, these results provide strong evidence that the accumulation of nitrate and ammonia in leaves is an early response of both woody and herbaceous plants to P deprivation. The data are consistent with the hypothesis that increased de novo arginine biosynthesis in leaves during P deficiency is in response to ammonia content of the leaves.We have recently demonstrated that the accumulation of arginine in the leaves of four citrus rootstock cultivars 'Supported by a grant from the Chancellor's Patent Funds awarded to ER, by a grant from the Academic Senate of the University of California to CJL, and by the Agricultural Experiment Station of the University of California, Riverside, CA 92521. 2The work presented here is also submitted in partial fulfillment of requirements for ...

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Accumulation of Arginine in Plants Afflicted with Iron-Deficiency Type Chlorosis

Cited by 29 publications

References 12 publications

The Accumulation of L-Ornithine in Halo-Blight Infected Bean Plants (Phaseolus vulgaris L.), Induced by the Toxin of the Pathogen Pseudomonas phaseolicola (Burkh.) Dowson

The Accumulation of L-Ornithine in Halo-Blight Infected Bean Plants (Phaseolus vulgaris L.), Induced by the Toxin of the Pathogen Pseudomonas phaseolicola (Burkh.) Dowson

The effects of ammonium on growth, accumulation of free amino acids and nutritional status of young phosphorus deficient Stratiotes aloides plants

Increased Arginine Biosynthesis during Phosphorus Deficiency

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