Accumulation of AGO2 Facilitates Tumorigenesis of Human Hepatocellular Carcinoma

Yang, Yang; Mei, Qi

doi:10.1155/2020/1631843

Cited by 3 publications

(2 citation statements)

References 26 publications

(36 reference statements)

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“…Indeed, five prognosis-related MALAT1 -interacting proteins, AGO2, HNRNPC, EZH2, SFPQ, and SRSF1 , have previously been linked to hepatotumorigenesis. AGO promotes cell proliferation and angiogenesis in HCC ( 27 , 28 ); silencing HNRNPC inhibits proliferation, migration, and invasion of HCC cells ( 29 ); EZH2 enhances protein kinase B activation to promote HCC progression ( 30 ); SFPQ plays an important role in the enhancement of fatty acid biosynthesis by NONO to promote HCC progression ( 31 ); and SRSF1 promotes HCC development, which can be regulated by MALAT1 ( 32 , 33 ).…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of long non-coding RNA MALAT1 in hepatocellular carcinoma: A study based on multi-omics analysis and RT-PCR validation

Liao¹,

Chen²,

Luo³

et al. 2023

Pathol. Oncol. Res.

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Background: This study aimed to explore the relationship between MALAT1 and the prognosis of patients with hepatocellular carcinoma (HCC).Methods: We constructed a MALAT1 protein-protein interaction network using the STRING database and a network of competing endogenous RNAs (ceRNAs) using the StarBase database. Using data from the GEPIA2 database, we studied the association between genes in these networks and survival of patients with HCC. The potential mechanisms underlying the relationship between MALAT1 and HCC prognosis were studied using combined data from RNA sequencing, DNA methylation, and somatic mutation data from The Cancer Genome Atlas (TCGA) liver cancer cohort. Tumor tissues and 19 paired adjacent non-tumor tissues (PANTs) from HCC patients who underwent radical resection were analyzed for MALAT1 mRNA levels using real-time PCR, and associations of MALAT1 expression with clinicopathological features or prognosis of patients were analyzed using log-rank test and Gehan-Breslow-Wilcoxon test.Results: Five interacting proteins and five target genes of MALAT1 in the ceRNA network significantly correlated with poor survival of patients with HCC (p < 0.05). High MALAT1 expression was associated with mutations in two genes leading to poor prognosis and may upregulate some prognostic risk genes through methylation. MALAT1 was significantly co-expressed with various signatures of genes involved in HCC progression, including the cell cycle, DNA damage repair, mismatch repair, homologous recombination, molecular cancer m6A, exosome, ferroptosis, infiltration of lymphocyte (p < 0.05). The expression of MALAT1 was markedly upregulated in HCC tissues compared with PANTs. In Kaplan-Meier analysis, patients with high MALAT1 expression had significantly shorter progression-free survival (PFS) (p = 0.033) and overall survival (OS) (p = 0.023) than those with low MALAT1 expression. Median PFS was 19.2 months for patients with high MALAT1 expression and 52.8 months for patients with low expression, while the corresponding median OS was 40.5 and 78.3 months. In subgroup analysis of patients with vascular invasion, cirrhosis, and HBsAg positive or AFP positive, MALAT1 overexpression was significantly associated with shorter PFS and OS. Models for predicting PFS and OS constructed based on MALAT1 expression and clinicopathological features had moderate predictive power, with areas under the receiver operating characteristic curves of 0.661–0.731. Additionally, MALAT1 expression level was significantly associated with liver cirrhosis, vascular invasion, and tumor capsular infiltration (p < 0.05 for all).Conclusion:MALAT1 is overexpressed in HCC, and higher expression is associated with worse prognosis. MALAT1 mRNA level may serve as a prognostic marker for patients with HCC after hepatectomy.

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Section: Discussionmentioning

confidence: 99%

Prognostic value of long non-coding RNA MALAT1 in hepatocellular carcinoma: A study based on multi-omics analysis and RT-PCR validation

Liao¹,

Chen²,

Luo³

et al. 2023

Pathol. Oncol. Res.

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“…The PAZ domain binds single-stranded nucleic acids, the PIWI domain has an RNase-H-like fold denoting its endonuclease activity, and Mid shows similarity to the MC domain, which binds to the mRNA cap and is required for efficient translation [ 148 ]. The functions of AGO2 in tumorigenesis are diverse and range from high levels associated with poor prognosis in HCC [ 149 ], ovarian carcinoma [ 150 ], and gastric cancer [ 151 ], or mediating the elevation of oncogenic miR-378a-3p in Burkitt lymphoma [ 152 ], to its low expression as an indicator of poor prognosis in CRC patients [ 153 ]. AGO2 protein–protein interactions (e.g., KRAS and AGO2) are involved in the progression of pancreatic ductal adenocarcinoma [ 154 ] and NSCLC [ 155 ].…”

Section: Cancer-associated Dna/rna Binding Proteinsmentioning

confidence: 99%

DNA and RNA Binding Proteins: From Motifs to Roles in Cancer

Bonczek

Wang

Gnanasundram

et al. 2022

IJMS

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DNA and RNA binding proteins (DRBPs) are a broad class of molecules that regulate numerous cellular processes across all living organisms, creating intricate dynamic multilevel networks to control nucleotide metabolism and gene expression. These interactions are highly regulated, and dysregulation contributes to the development of a variety of diseases, including cancer. An increasing number of proteins with DNA and/or RNA binding activities have been identified in recent years, and it is important to understand how their activities are related to the molecular mechanisms of cancer. In addition, many of these proteins have overlapping functions, and it is therefore essential to analyze not only the loss of function of individual factors, but also to group abnormalities into specific types of activities in regard to particular cancer types. In this review, we summarize the classes of DNA-binding, RNA-binding, and DRBPs, drawing particular attention to the similarities and differences between these protein classes. We also perform a cross-search analysis of relevant protein databases, together with our own pipeline, to identify DRBPs involved in cancer. We discuss the most common DRBPs and how they are related to specific cancers, reviewing their biochemical, molecular biological, and cellular properties to highlight their functions and potential as targets for treatment.

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New findings on the action of hypericin in hypoxic cancer cells with a focus on the modulation of side population cells

Buľková¹,

Vargová²,

Babinčák³

et al. 2023

Biomedicine & Pharmacotherapy

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Accumulation of AGO2 Facilitates Tumorigenesis of Human Hepatocellular Carcinoma

Cited by 3 publications

References 26 publications

Prognostic value of long non-coding RNA MALAT1 in hepatocellular carcinoma: A study based on multi-omics analysis and RT-PCR validation

Prognostic value of long non-coding RNA MALAT1 in hepatocellular carcinoma: A study based on multi-omics analysis and RT-PCR validation

DNA and RNA Binding Proteins: From Motifs to Roles in Cancer

New findings on the action of hypericin in hypoxic cancer cells with a focus on the modulation of side population cells

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