Accumulation of a Brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine

Altenbach, Susan B.; Kuo, Chiung-Chi; Staraci, Lisa C.; Pearson, Karen W.; Wainwright, Connie; Georgescu, Anca Meda; Townsend, Jeffrey A.

doi:10.1007/bf00034952

Cited by 187 publications

(90 citation statements)

References 23 publications

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“…6, using the known amount of purified Brazil nut 2S albumin in lane 1 as a standard, indicates that the transgenic plant lines used in that experiment of types 14, 15 and 16 express the transgenic proteins at 0.30, 0.16 and 0.27% of the total extractable proteins. This expression level is better than that obtained with chimeric Brazil nut 2S genes in B. napus [6], but less than the levels with modified Arabidopsis 2S albumins expressed in Arabidopsis [22], and the levels (1.74%) obtained by Altenbach et al [24] in B. napus. Part of the difference may be due to our use of the relatively constitutive 35S promoter instead of a strong seed promoter.…”

Section: Protein Analysiscontrasting

confidence: 57%

Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants

Marcellino¹,

Neshich²,

Sá³

et al. 1996

FEBS Letters

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Brazil nut 2S albumins lack the essential amino acid tryptophan. In order to improve the protein's nutritional value and create a basis for structural investigations, three separate modified Brazil nut 2S albumin genes were constructed. The first mutant contains five consecutive tryptophan codons, while the other two modified genes encode proteins carrying single tryptophan residues at sites that will allow confirmation of the predicted protein structure through fluorescence quenching techniques. The modified genes, under the regulation of the CaMV 35S promoter, were introduced into Nicotiana tabacum. All three modified genes were correctly transcribed and the 2S albumin accumulated in the seeds of transgenic plants.

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Section: Protein Analysiscontrasting

confidence: 57%

Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants

Marcellino¹,

Neshich²,

Sá³

et al. 1996

FEBS Letters

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“…Quality breeding of oilseed rape has been largely orientated by nutritional concerns driven by consumer and food industry demands. Several promising attempts have been made in rapeseed to increase cysteine, methionine and lysine contents, with these two latter amino acids being essential (Altenbach et al, 1992). Current goals for improving seed quality in rapeseed deal with the development of yellow-seeded cultivars that are high yielding and display higher levels of oil and proteins.…”

Section: Total Phenolic Contentsmentioning

confidence: 99%

Variations in fatty acid composition, glucosinolate profile and some phytochemical contents in selected oil seed rape (<i>Brassica napus</i> L.) cultivars

El-Beltagi¹,

Mohamed²

2010

Grasas y Aceites

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Rapeseed (Brassica napus L.) is now the third most important source of edible oil in the world after soybean and palm oil. In this study seeds of five different rapeseed cultivars namely; pactol, silvo, topas, serw 4 and serw 6 were evaluated for their fatty acid composition, glucosinolate profile, amino acids, total tocopherols and phenolic content. Among all cultivars significant variability in fatty acids were observed. The oleic acid (C18:1) ranged from 56.31% to 58.67%, linoleic acid (C18:2) from 10.52% to 13.74%, α-linolenic acid (C18:3) from 8.83% to 10.32% and erucic acid (22:1) from 0.15% to 0.91%. The glucosinolate profile of rapeseed was also separated and identified using high-performance liquid chromatography. Small variations in the glucosinolate profile were observed among all tested cultivars; however, progoitrin and gluconapin were the major glucosinolate found. Additionally, silvo cultivar showed the highest total glucosinolate c ontents (5.97 μmol/g dw). Generally, the contents of aspartic, glutamic, arginine and leucine were high, while the contents of tyrosine and isoleucine were low among all cultivars. For total tocopherols, the results indicated that both serw 6 and pactol cultivars had the highest total tocopherol contents (138.3 and 102.8 mg/100 g oil, respectively). Total phenolic contents varied from 28.0 to 35.4 mg/g dw. The highest total phenolic content was found in topas while the lowest value was detected in serw 6. These parameters; fatty acid contents, glucosinolate profile and amino acids together with total tocopherols and phenolic contents, could be taken into consideration by oilseed rape breeders as selection criteria for developing genotypes with modified seed quality traits in Brassica napus L.

La colza (Brassica napus L.) es hoy en día el tercer cultivo más importante de aceites comestibles en el mundo tras el aceite de soja y de palma. En este estudio semillas de cinco cultivos diferentes de colza, denominadas pactol, silvo, topas, serw 4 y serw 6, fueron evaluadas mediante su composición en ácidos grasos, perfil de glucosinolato, aminoácidos, tocoferoles totales y contenido fenólico. En todos los cultivos, una significativa variabilidad en los ácidos grasos fue observada. El ácido oleico (C18:1) vario desde un 56.31% a un 58.67%, ácido linoleico (C18:2) desde un 10.52% a un 13.74%, ácido α-linolenic (C18:3) desde 8.83% a un 10.32% y ácido erúcico (22:1) desde un 0.15% a un 0.91%. El perfil de glucosinolatos de colza fue también separado e identificado usando cromatografía líquida de alta eficacia. Una baja variación en el perfil de glucosinolato fue observado en todos los cultivos ensayados; sin embargo, progoitrina y gluconapina fueron los principales glucosinolatos encontraos en todos los cultivos. Además, el cultivo silvo mostro el contenido de glucosinolatos totales más alto (5.97 μmol/g dw). Generalmente, el contenido de aspártico, glutámico, arginina y leucina fue alto, mientras que el contenido de tirosina y isoleucina fue bajo en tod...

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“…Typical 2S albumins, such as the napins from the Brassicaceae or the Brazil nut 2S albumin Ber e 1, are heterodimeric proteins consisting of two subunits of approximately 4 and 9 kDa, which are held together by conserved interchain disulfide bonds [13]. In addition to their biological interest, 2S albumins have been used by means of genetic engineering as carriers for the synthesis of biologically active peptides as well as for improving the nutritional properties of grain crops by increasing their content of essential amino acids [16].…”

Section: S Albuminsmentioning

confidence: 99%

Molecular Properties of Plant Food Allergens: A Current Classification into Protein Families

Hauser¹,

Egger²,

Wallner³

et al. 2008

TOIJ

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So far the allergen list of the International Union of Immunological Societies (IUIS) allergen nomenclature subcommittee comprises 130 plant-derived food allergens. Based on sequence homology these allergens can be classified into only 27 out of 9,000 known protein families according to the Allfam database. These families comprise the prolamin and cupin superfamilies, pathogenesis related proteins, profilins, thaumatin-like proteins, oleosins, expansins, a number of enzymes and protease inhibitors among others. The classification on structural and thus biochemical and functional similarities will provide a new outlook on the molecules and might contribute to answer the question about allergenicity of different proteins. This will help to define clinical relevant allergenic molecules and explain cross-reactive phenomena between single food allergen sources as well as between food allergens and allergenic molecules of other origins (e.g. pollen).

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Accumulation of a Brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine

Cited by 187 publications

References 23 publications

Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants

Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants

Variations in fatty acid composition, glucosinolate profile and some phytochemical contents in selected oil seed rape (<i>Brassica napus</i> L.) cultivars

Molecular Properties of Plant Food Allergens: A Current Classification into Protein Families

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