In this study we try to evaluate the accumulation of heavy metals, including Pb, Zn, Cu and Cr in the soil, the entry into plants, and the effect on the physiological and biochemical processes of olive tree. We try to study the contamination of the leaves, stem and roots of olive trees collected from 24 sampling sites throughout three roads in the region of Sfax, Tunisia. To better study this contamination, we used two contamination indices, the accumulation and translocation factor. For Gremda road, Pb and Zn contamination does not exceed 50m. For Tunis road, contamination of leaves stems and roots by Pb and Cr arrived up to 50m in the eastern and western side of the road. In contrast, Zn contamination reached 50m in the eastern and 25m in the western side of road. Likewise, metal contamination affects the roots and leaves of Manzel Chaker olive trees at a distance of 3m but this contamination does not affect the physiological and biochemical mechanisms of the latter. The contamination of sites near Tunis and Gremda roads affects the chlorophyll assimilation of olive trees planted in these sites but, the chlorophyll contents of Manzel chaker sites are less affected. On the other hand, for soluble sugars, the olive leaves close to Manzel Chaker and Tunis roads recorded higher sugar contents than those of Gremda sites. The leaves of olive trees in the sites close to Gremda and Tunis roads showed a weakened antioxidant activity compared to that recorded in the control sites.