Accumulation and Transport of 1-Aminocyclopropane-1-Carboxylic Acid (ACC) in Plants: Current Status, Considerations for Future Research and Agronomic Applications

Vanderstraeten, Lisa; Straeten, Dominique Van Der

doi:10.3389/fpls.2017.00038

Cited by 86 publications

(80 citation statements)

References 172 publications

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“…They are also able to conjugate ACC with glutathione to γ-glutamyl-ACC (GACC) and with jasmonic acid to JA-ACC to control the ACC content. Additionally, plants can degrade ACC irreversibly by an ACC deaminase α-ketobutyrate (for reviews about ACC content regulation see Van de Poel and Van Der Straeten (2014), Le Deunff and Lecourt (2016), and Vanderstraeten and Van Der Straeten (2017)). To date neither the contribution of each of these ACC degradation pathways to control the ACC pool nor their interplay has been studied, yet.…”

Section: Discussionmentioning

confidence: 99%

AtDAT1 is a key enzyme of D-amino acid stimulated ethylene production inArabidopsis thaliana

Suárez

Hener

Lehnhardt

et al. 2019

Preprint

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Keywords: D-amino acids in plants, D-amino acid-stimulated ethylene production, D-amino acid 9 specific transaminase, D-methionine, 1-aminocyclopropane-1-carboxylic acid, ethylene, amino acid 10 malonylation 11 Manuscript type: Original research 12Abstract 15 D-enantiomers of proteinogenic amino acids (D-AAs) are found ubiquitously, but the knowledge 16 about their metabolism and functions in plants is scarce. A long forgotten phenomenon in this regard 17 is the D-AA-stimulated ethylene production in plants. As a starting point to investigate this effect the 18Arabidopsis accession Landsberg erecta (Ler) got into focus as it was found defective in 19 metabolizing D-AAs. Combining genetics and molecular biology of T-DNA lines and natural 20 variants together with biochemical and physiological approaches we could identify AtDAT1 as a 21 major D-AA transaminase in Arabidopsis. Atdat1 loss-of-function mutants and Arabidopsis 22 accessions with defective AtDAT1 alleles were not able to produce D-Ala, D-Glu and L-Met, the 23 metabolites of D-Met, anymore. This result corroborates the biochemical characterization of 24AtDAT1, which showed highest activity using D-Met as substrate. Germination of seedlings in light 25and dark led to enhanced growth inhibition of atdat1 mutants on D-Met. Ethylene measurements 26revealed an enhanced D-AA stimulated ethylene production in these mutants. According to initial 27 working models of this phenomenon D-Met is preferentially malonylated instead of the ethylene 28 precursor 1-aminocyclopropane-1-carboxylic acid (ACC). This decrease of ACC degradation should 29 then lead to the increase of ethylene production. We could observe in our studies a reciprocal relation 30 of malonylated methionine and ACC upon D-Met application and even significantly more malonyl-31 methionine in atdat1 mutants. Unexpectedly, the malonyl-ACC levels did not differ between mutants 32 and wild type in these experiments. With AtDAT1, the first central enzyme of plant D-AA 33 metabolism was characterized biochemically and physiologically. The specific effects of D-Met on 34 ACC metabolization, ethylene production and plant development of dat1 mutants unraveled the 35 impact of AtDAT1 on these processes, but they are not in full accordance to previous working 36 AtDAT1 regulates ethylene production 2 models. Instead, our results imply the influence of additional candidate factors or processes on D-37 AA-stimulated ethylene production which await to be uncovered. 38

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Section: Discussionmentioning

confidence: 99%

AtDAT1 is a key enzyme of D-amino acid stimulated ethylene production inArabidopsis thaliana

Suárez

Hener

Lehnhardt

et al. 2019

Preprint

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“…As mentioned above, D-Met may affect the levels of ACC and all its derivatives. It has been shown before that ACC itself acts as a signaling molecule and the same is also discussed for its derivatives (for reviews, see Van de Poel and Van Der Straeten, 2014;Vanderstraeten and Van Der Straeten, 2017;Nascimento et al, 2018). D-Met accumulation leads to an increase of ethylene concentrations, but possibly other compounds like ACC and its derivatives may also contribute to the observed physiological responses of dat1 affected plants.…”

Section: Discussionmentioning

confidence: 94%

“…There is also the possibility that ACC is conjugated with glutathione to g-glutamyl-ACC (GACC) and with jasmonic acid to JA-ACC to control the ACC homeostasis. Additionally, plants can irreversibly degrade ACC to a-ketobutyrate by an ACC deaminase [for reviews about ACC content regulation, see Van de Poel and Van Der Straeten (2014); Le Deunff and Lecourt (2016), and Vanderstraeten and Van Der Straeten (2017)]. To date, neither the contribution of each of these ACC catabolic pathways nor their interplay for the control of ACC homeostasis have been studied, yet.…”

Section: Discussionmentioning

confidence: 99%

AtDAT1 Is a Key Enzyme of D-Amino Acid Stimulated Ethylene Production in Arabidopsis thaliana

et al. 2019

View full text Add to dashboard Cite

D-Enantiomers of proteinogenic amino acids (D-AAs) are found ubiquitously, but the knowledge about their metabolism and functions in plants is scarce. A long forgotten phenomenon in this regard is the D-AA-stimulated ethylene production in plants. As a starting point to investigate this effect, the Arabidopsis accession Landsberg erecta (Ler) got into focus as it was found defective in metabolizing D-AAs. Combining genetics and molecular biology of T-DNA insertion lines and natural variants together with biochemical and physiological approaches, we could identify AtDAT1 as a major D-AA transaminase in Arabidopsis. Atdat1 loss-of-function mutants and Arabidopsis accessions with defective AtDAT1 alleles were unable to produce the metabolites of D-Met, D-Ala, D-Glu, and L-Met. This result corroborates the biochemical characterization, which showed highest activity of AtDAT1 using D-Met as a substrate. Germination of seedlings in light and dark led to enhanced growth inhibition of atdat1 mutants on D-Met. Ethylene measurements revealed an increased D-AA stimulated ethylene production in these mutants. According to initial working models of this phenomenon, D-Met is preferentially malonylated instead of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). This decrease of ACC degradation should then lead to the increase of ethylene production. We could observe a reciprocal relation of malonylated methionine and ACC upon D-Met application and significantly more malonyl-methionine in atdat1 mutants. Unexpectedly, the malonyl-ACC levels did not differ between mutants and wild type. With AtDAT1, the first central enzyme of plant D-AA metabolism was characterized biochemically and physiologically. The specific effects of D-Met on ACC metabolism, ethylene production, and plant development of dat1 mutants unraveled the impact of AtDAT1 on these processes; however, they are not in full accordance to previous working models. Instead, our results imply the influence of additional factors or processes on D-AA-stimulated ethylene production, which await to be uncovered.

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“…Ethylene biosynthesized in plants occurs via a well-defined pathway in which ACC synthase (ACS) catalyses the conversion of S-adenosyl-L-methionine (SAM) to ACC, which is then converted to ethylene by ACC oxidase (ACO) 37 . ACS-catalysed ACC formation is thought to be the primary regulatory step in ethylene biosynthesis 60 . Based on the maize B73 reference genome sequence (Version 5b.60) (http://ensembl.…”

Section: Discussionmentioning

confidence: 99%

Identification of Quantitative Trait Loci Controlling Ethylene Production in Germinating Seeds in Maize (Zea mays L.)

Jia

Wang

et al. 2020

Sci Rep

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Plant seed germination is a crucial developmental event that has significant effects on seedling establishment and yield production. This process is controlled by multiple intrinsic signals, particularly phytohormones. The gaseous hormone ethylene stimulates seed germination; however, the genetic basis of ethylene production in maize during seed germination remains poorly understood. In this study, we quantified the diversity of germination among 14 inbred lines representing the parental materials corresponding to multiple recombinant inbred line (RIL) mapping populations. Quantitative trait loci (QTLs) controlling ethylene production were then identified in germinating seeds from an RIL population constructed from two parental lines showing differences in both germination speed and ethylene production during germination. To explore the possible genetic correlations of ethylene production with other traits, seed germination and seed weight were evaluated using the same batch of samples. On the basis of high-density single nucleotide polymorphism-based genetic linkage maps, we detected three QTLs for ethylene production in germinating seeds, three QTLs for seed germination, and four QTLs for seed weight, with each QTL explaining 5.8%-13.2% of the phenotypic variation of the trait. No QTLs were observed to be co-localized, suggesting that the genetic bases underlying the three traits are largely different. Our findings reveal three chromosomal regions responsible for ethylene production during seed germination, and provide a valuable reference for the future investigation of the genetic mechanism underlying the role of the stress hormone ethylene in maize germination control under unfavourable external conditions. Maize (Zea mays L.) is one of the most important food crops worldwide and is widely used as genetic research material for studying various traits. Seed germination is a developmental event that is crucial for plant propagation. Uniform seed germination and seedling emergence are prerequisites for high yield production in corn. Germination is initiated following the imbibition of water by quiescent dry seeds and is completed following the protrusion of the radicle through the seed coat and endosperm 1 . This process is regulated by sophisticated endogenous plant components as well as environmental factors 2-6 .Intensive exploration of the mechanism underlying germination in the model plant Arabidopsis (Arabidopsis thaliana) has revealed the pivotal roles of plant hormones and other signalling molecules in this complicated process 2,4,5,7-11 . Although germination in crop species has not received as much attention as that in Arabidopsis, early findings in maize using mutants that are deficient in or insensitive to abscisic acid (ABA) 12-14 have facilitated the establishment of a major concept in germination: that the balance between ABA and bioactive gibberellins (GA) and not the actual amount of each hormone is the primary factor that determines seed germination vigor 2,5,15,16 . Post-genomic technologies ...

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Accumulation and Transport of 1-Aminocyclopropane-1-Carboxylic Acid (ACC) in Plants: Current Status, Considerations for Future Research and Agronomic Applications

Cited by 86 publications

References 172 publications

AtDAT1 is a key enzyme of D-amino acid stimulated ethylene production inArabidopsis thaliana

AtDAT1 is a key enzyme of D-amino acid stimulated ethylene production inArabidopsis thaliana

AtDAT1 Is a Key Enzyme of D-Amino Acid Stimulated Ethylene Production in Arabidopsis thaliana

Identification of Quantitative Trait Loci Controlling Ethylene Production in Germinating Seeds in Maize (Zea mays L.)

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