Accumulation and spatial distribution of arsenic and phosphorus in the fern Pityrogramma calomelanos evaluated by micro X-ray fluorescence spectrometry

Campos, Naiara Viana; Guerra, Marcelo Braga Bueno; Mello, Jaime Wilson Vargas de; Schaefer, Carlos Ernesto Gonçalves Reynaud; Krug, Francisco José; Alves, Elton Eduardo Novais; Azevedo, Aristéa Alves

doi:10.1039/c5ja00348b

Cited by 22 publications

(15 citation statements)

References 43 publications

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“…50 mg) in closed TFM ® digestion vessels (ETHOS 1600, Milestone, Italy) before adding 5.0 ml of 2.8 M HNO 3 and 2.0 ml of 30% H 2 O 2 (v/v). Microwave‐assisted digestions and As and P determinations in an inductively coupled plasma atomic emission spectrometer (ICP OES; iCAP 6500 Duo, Thermo Scientific, Waltham, MA, USA) were performed as described by Campos et al ().…”

Section: Methodsmentioning

confidence: 99%

Arsenic hyperaccumulation induces metabolic reprogramming in Pityrogramma calomelanos to reduce oxidative stress

Campos

Araújo

Arcanjo-Silva

et al. 2016

Physiologia Plantarum

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Arsenic (As) pollution is a major environmental concern due to its worldwide distribution and high toxicity to organisms. The fern Pityrogramma calomelanos is one of the few plant species known to be able to hyperaccumulate As, although the mechanisms involved are largely unknown. This study aimed to investigate the metabolic adjustments involved in the As-tolerance of P. calomelanos. For this purpose, ferns with five to seven fronds were exposed to a series of As concentrations. Young fronds were used for biochemical analysis and metabolite profiling using gas chromatography-mass spectrometry. As treatment increased the total concentration of proteins and soluble phenols, enhanced peroxidase activities, and promoted disturbances in nitrogen and carbon metabolism. The reduction of the glucose pool was one of the striking responses to As. Remarkable changes in amino acids levels were observed in As-treated plants, including those related to biosynthesis of glutathione and phenols, osmoregulation and two photorespiratory intermediates. In addition, increases in polyamines levels and antioxidant enzyme activities were observed. In summary, this study indicates that P. calomelanos tolerates high concentration of As due to its capacity to upregulate biosynthesis of amino acids and antioxidants, without greatly disturbing central carbon metabolism. At extremely high As concentrations, however, this protective mechanism fails to block reactive oxygen species production, leading to lipid peroxidation and leaf necrosis.

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Section: Methodsmentioning

confidence: 99%

Arsenic hyperaccumulation induces metabolic reprogramming in Pityrogramma calomelanos to reduce oxidative stress

Campos

Araújo

Arcanjo-Silva

et al. 2016

Physiologia Plantarum

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“…The determination of the spatial distribution of elements can show possible synergism or antagonism between the potentially toxic elements that one intends to remove from soil and the mineral nutrients in the plant ( Bueno Guerra et al, 2013 ). Imaging techniques can be used to evaluate such interactions ( Campos et al, 2015 ). The main techniques currently used are LA-ICP-MS ( Tian et al, 2011 ), μ-XRF ( Campos et al, 2015 ), synchrotron XRF ( Hokura et al, 2006 ), and XRF microtomography ( Terzano et al, 2008 ).…”

Section: Resultsmentioning

confidence: 99%

Laboratory Microprobe X-Ray Fluorescence in Plant Science: Emerging Applications and Case Studies

et al. 2018

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In vivo and micro chemical analytical methods have the potential to improve our understanding of plant metabolism and development. Benchtop microprobe X-ray fluorescence spectroscopy (μ-XRF) presents a huge potential for facing this challenge. Excitation beams of 30 μm and 1 mm in diameter were employed to address questions in seed technology, phytopathology, plant physiology, and bioremediation. Different elements were analyzed in several situations of agronomic interest: (i) Examples of μ-XRF yielding quantitative maps that reveal the spatial distribution of zinc in common beans (Phaseolus vulgaris) primed seeds. (ii) Chemical images daily recorded at a soybean leaf (Glycine max) infected by anthracnose showed that phosphorus, sulfur, and calcium trended to concentrate in the disease spot. (iii) In vivo measurements at the stem of P. vulgaris showed that under root exposure, manganese is absorbed and transported nearly 10-fold faster than iron. (iv) Quantitative maps showed that the lead distribution in a leaf of Eucalyptus hybrid was not homogenous, this element accumulated mainly in the leaf border and midrib, the lead hotspots reached up to 13,400 mg lead kg-1 fresh tissue weight. These case studies highlight the ability of μ-XRF in performing qualitative and quantitative elemental analysis of fresh and living plant tissues. Thus, it can probe dynamic biological phenomena non-destructively and in real time.

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“…Laser‐induced breakdown spectroscopy (LIBS) is another complementary tool that presents promising features aiming at the direct analysis of solid samples. It is fast, multielemental, allows simultaneous determinations, and the instruments are also available as portable devices …”

Section: Introductionmentioning

confidence: 99%

Direct determination of mineral nutrients in soybean leaves under vivo conditions by portable X‐ray fluorescence spectroscopy

et al. 2019

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A portable energy dispersive X‐ray fluorescence (XRF) spectrometer furnished with an Rh X‐ray tube was evaluated for the determination of macronutrients and micronutrients in soybean leaves (Glycine max L.). XRF instrumental parameters were optimized in a univariate way, and emission intensities were measured for 60 s and under vacuum for macronutrients, and during 180 s, under air, and 305 μm Al/25.4 μm Ti filter, for micronutrients. Fresh and dried leaves were irradiated, and it was possible to identify P, K, Ca, S, Mn, Fe, Cu, and Zn Kα emission lines. For comparative purpose, the samples were also microwave assisted, digested and analyzed by inductively coupled plasma optical emission spectrometry. In general, linear correlations between K, Ca, Mn, Fe, Cu, and Zn concentrations in the tested samples and the corresponding portable XRF (pXRF) intensities were obtained. The linear correlation coefficients (R2) ranged from 0.42 to 0.86. In addition, the detection limits were suitable for plant nutrient diagnosis. It is demonstrated that pXRF is a simple and powerful tool for analysis of plant materials.

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Accumulation and spatial distribution of arsenic and phosphorus in the fern Pityrogramma calomelanos evaluated by micro X-ray fluorescence spectrometry

Abstract: μ-EDXRF is a strong analytical tool enabling the simultaneous mapping of As and P in the As-hyperaccumulator fern Pityrogramma calomelanos.

Cited by 22 publications

References 43 publications

Arsenic hyperaccumulation induces metabolic reprogramming in Pityrogramma calomelanos to reduce oxidative stress

Arsenic hyperaccumulation induces metabolic reprogramming in Pityrogramma calomelanos to reduce oxidative stress

Laboratory Microprobe X-Ray Fluorescence in Plant Science: Emerging Applications and Case Studies

Direct determination of mineral nutrients in soybean leaves under vivo conditions by portable X‐ray fluorescence spectroscopy

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