Accumulation and Distribution of Fluorescent Microplastics in the Early Life Stages of Zebrafish

Xu, Chao; Guo, Hangqin; Wang, Ruijiong; Li, Tianyang; Gu, Linqi; Sun, Liwei

doi:10.3791/62117

Cited by 1 publication

(1 citation statement)

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“…The diameter of the yolk sac is calculated using an ocular micrometer fitted to the microscope, as described previously [23]. To assess the presence of edema in the sac, a Nikon microscope, DS-Fi1, Kyoto, Japan was employed to manually record relevant information.…”

Section: Analysis Of Yolk Sac Edemamentioning

confidence: 99%

Aspartame Causes Developmental Defects and Teratogenicity in Zebra Fish Embryo: Role of Impaired SIRT1/FOXO3a Axis in Neuron Cells

Pandaram,

Paul,

Wankhar

et al. 2024

Biomedicines

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Aspartame, a widely used artificial sweetener, is present in many food products and beverages worldwide. It has been linked to potential neurotoxicity and developmental defects. However, its teratogenic effect on embryonic development and the underlying potential mechanisms need to be elucidated. We investigated the concentration- and time-dependent effects of aspartame on zebrafish development and teratogenicity. We focused on the role of sirtuin 1 (SIRT1) and Forkhead-box transcription factor (FOXO), two proteins that play key roles in neurodevelopment. It was found that aspartame exposure reduced the formation of larvae and the development of cartilage in zebrafish. It also delayed post-fertilization development by altering the head length and locomotor behavior of zebrafish. RNA-sequencing-based DEG analysis showed that SIRT1 and FOXO3a are involved in neurodevelopment. In silico and in vitro analyses showed that aspartame could target and reduce the expression of SIRT1 and FOXO3a proteins in neuron cells. Additionally, aspartame triggered the reduction of autophagy flux by inhibiting the nuclear translocation of SIRT1 in neuronal cells. The findings suggest that aspartame can cause developmental defects and teratogenicity in zebrafish embryos and reduce autophagy by impairing the SIRT1/FOXO3a axis in neuron cells.

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Section: Analysis Of Yolk Sac Edemamentioning

confidence: 99%