Accumulation and biotransformation of chitosan-modified selenium nanoparticles in exposed radish (Raphanus sativus)

Palomo-Siguero, María; López-Heras, Maria Isabel; Cámara, Carmen; Madrid, Yolanda

doi:10.1039/c4ja00407h

Cited by 31 publications

(23 citation statements)

References 18 publications

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“…To understand the adverse or beneficial effects of NPs in biological samples, studies are carried out by using a decomposition method for the samples to quantify the elemental content in a given NP through ICP‐MS. On the contrary, for specific information regarding NP morphology and biotransformation in biological samples, additional techniques coupled or not to ICP‐MS, discussed in the next section, are required …”

Section: Icp‐ms For Elemental Quantification Of Nanoparticlesmentioning

confidence: 99%

“…iquid chromatography coupled to ICP‐MS (LC‐ICP‐MS) has been extensively used for evaluating metallobiospecies in several organisms because such a strategy can provide elemental information that is related to molecular information . From a nanometallomics point of view, it is important to monitor and evaluate the NPs and their effects because they exhibit different properties of ionic and microscale materials .…”

Section: Np Evaluation Using Separation Techniques Coupled To Icp‐msmentioning

confidence: 99%

“…From a nanometallomics point of view, it is important to monitor and evaluate the NPs and their effects because they exhibit different properties of ionic and microscale materials . Although only a few studies report on the evaluation of NPs using LC‐ICP‐MS, such a strategy combined with high‐resolution mass spectrometry (HR‐MS) is a valuable alternative to obtain more information regarding NP biotransformation and its effects in a given organism. In fact, the NPs can be monitored and separated according to their size using LC‐ICP‐MS, as well as NPs can be distinguished by their free ions.…”

Section: Np Evaluation Using Separation Techniques Coupled To Icp‐msmentioning

confidence: 99%

“…Palomo‐Siguero et al used LC‐ICP‐MS to evaluate the biotransformation of chitosan‐modified SeNPs (CS‐SeNPs) and their effects in radish plants. For comparison, radish plants were grown in hydroponic media in the presence of spherical CS‐SeNPs, with an average diameter of 26 ± 3 nm, and selenite as a source of selenium (Se).…”

Section: Np Evaluation Using Separation Techniques Coupled To Icp‐msmentioning

confidence: 99%

“…However, differences in Se accumulation from CS‐SeNPs and selenite groups were observed, because the radish plants treated with selenite absorbed an additional 25% Se. Despite this fact, no toxic symptoms were noticed in the plants grown in both CS‐SeNPs and selenite, because after CS‐SeNPs are accumulated, they are biotransformed into MeSeCys—a non‐proteinogenic Se amino acid related to the Se detoxification pathway in plants . In fact, this approach based on LC‐ICP‐MS is ideal for evaluating the effects of NPs in a system, because LC/ICP‐MS provides information regarding NP biotransformation, thus enabling the prediction of the behavior of NPs in a given organism.…”

Section: Np Evaluation Using Separation Techniques Coupled To Icp‐msmentioning

confidence: 99%

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Inductively coupled plasma mass spectrometry based platforms for studies involving nanoparticle effects in biological samples

Galazzi

Chacón-Madrid

Freitas

et al. 2020

Rapid Comm Mass Spectrometry

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The widespread application of nanoparticles (NPs) in recent times has caused concern because of their effects in biological systems. Although NPs can be produced naturally, industrially synthesized NPs affect the metabolism of a given organism because of their high reactivity. The biotransformation of NPs involves different processes, including aggregation/agglomeration, and reactions with biomolecules that will be reflected in their toxicity. Several analytical techniques, including inductively coupled plasma mass spectrometry (ICP‐MS), have been used for characterizing and quantifying NPs in biological samples. In fact, in addition to providing information regarding the morphology and concentration of NPs, ICP‐MS‐based platforms, such as liquid chromatography/ICP‐MS, single‐particle ICP‐MS, field‐flow fractionation (asymmetrical flow field‐flow fractionation)‐ICP‐MS, and laser ablation‐ICP‐MS, yield elemental information about molecules. Furthermore, such information together with speciation analysis enlarges our understanding of the interaction between NPs and biological organisms. This study reports the contribution of ICP‐MS‐based platforms as a tool for evaluating NPs in distinct biological samples by providing an additional understanding of the behavior of NPs and their toxicity in these organisms.

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Section: Icp‐ms For Elemental Quantification Of Nanoparticlesmentioning

confidence: 99%

Section: Np Evaluation Using Separation Techniques Coupled To Icp‐msmentioning

confidence: 99%

Section: Np Evaluation Using Separation Techniques Coupled To Icp‐msmentioning

confidence: 99%

Section: Np Evaluation Using Separation Techniques Coupled To Icp‐msmentioning

confidence: 99%

Section: Np Evaluation Using Separation Techniques Coupled To Icp‐msmentioning

confidence: 99%

See 3 more Smart Citations

Inductively coupled plasma mass spectrometry based platforms for studies involving nanoparticle effects in biological samples

Galazzi

Chacón-Madrid

Freitas

et al. 2020

Rapid Comm Mass Spectrometry

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Nanospeciation Analysis Using Field Flow Fractionation

Moreno-Martín

Sanz‐Landaluze

Madrid

2017

Encyclopedia of Analytical Chemistry

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Trace element speciation is currently moving toward nanotechnology field. Nanosized materials such as metallic nanoparticles (MNPs) and metallic oxide nanoparticles (MONPs) are being used in many fields. However, their impact on the environment and human health is still poorly understood. Characterizing metal and metalloids in nanosize form is not an easy task, and it is still at an incipient stage of development. There is a current trend of using multimethod approaches to provide information, especially for nano‐objects in complex matrices. Field flow fractionation (FFF) techniques, especially asymmetrical flow‐field flow fractionation (AF 4 ), have been shown as a promising approach for size‐based elemental speciation. This article provides a detailed description of the application of AF 4 to trace element speciation in nanosize form. The description will focus first on the difficulties encountered in MNPs and MONPs determination, especially those factors affecting their stability (sample treatment and interaction with living systems). The second part outlines the application of the AF 4 in nanospeciation consisting of a description on the basic principles of the technique and the parameters affecting MNPs and MONPs separation along with different methodologies for their sizing and quantification. The article presents throughout selected examples on the application of AF 4 for characterizing nanoparticles.

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Biophysical Methods of Detection and Quantification of Uptake, Translocation, and Accumulation of Nanoparticles

Medina-Velo

Zuverza‐Mena

Tan

et al. 2016

Plant Nanotechnology

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Accumulation and biotransformation of chitosan-modified selenium nanoparticles in exposed radish (Raphanus sativus)

Abstract: Biotransformation of chitosan-modified SeNPs (CS-SeNPs) in radish plants was investigated by using high performance liquid chromatography (HPLC) and asymmetrical flow field flow fractionation (AF4) on line coupled to inductively coupled plasma mass spectrometry (ICP-MS) as well as TEM.

Cited by 31 publications

References 18 publications

Inductively coupled plasma mass spectrometry based platforms for studies involving nanoparticle effects in biological samples

Inductively coupled plasma mass spectrometry based platforms for studies involving nanoparticle effects in biological samples

Nanospeciation Analysis Using Field Flow Fractionation

Biophysical Methods of Detection and Quantification of Uptake, Translocation, and Accumulation of Nanoparticles

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