Accretion and turnover of RNA in the renoprival kidney

Ra, Malt; Da, Lemaitre

doi:10.1152/ajplegacy.1968.214.5.1041

Cited by 62 publications

(31 citation statements)

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“…Our values for DNA and RNA concentrations are slightly greater than those reported by others for 42-day-old mice 15 days after nephrectomy (35). Similarly, the increase in RNA:DNA ratio is not as dramatic as that in older mice (35).…”

Section: Discussioncontrasting

confidence: 79%

Compensatory Renal Growth in the Mouse. II. The Effect of Growth Hormone Deficiency

et al. 1981

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Summarysuppressed (21,32,49). Three days after nephrectomy in hypophysectomized rats, tritiated thymidine shows that mitoses are still Allometry was used to study the effect of growth hormone (GH) 60% as frequent as normal and GH only partially restores this deficiency on compensatory renal growth (CRG) in a dwarf mouse deficiency (32), strain (Little). Nucleic acid and protein estimations were used to study of the role of GH is complicated by the postulated assess changes in cellular hyperplasia and hypertrophy. Nephrec-existence of a GH-related "renotrophic factorn (18), for which tomy was ~erformed at 5,15, or 35 days of age with removal of the there is accumulating evidence in vitro (39,43) Of because of its similarity to somatomedin (41) (a proposed "second age was closely related to that of the normal heteroz~gote (lit/ messengern for GH (24), but nonsuppresslble, insulin-like activity +). A regression line for the renoprival kidneys in lit/lit animals actually inhibited CRG in hypophysectomized rats, was parallel to that of the rightStudying the role of GH is further complicated by problems interval between the regression lines was equivalent to a constant that hypophysectomy creates in animal models, One of these is difference of approximately "% between reno~riva' and the ensuing severity of weight loss. Difficulties due to a relatively right kidneys and was similar to that found in the normal hetero-greater loss of kidney weight (KW) (42) in hypophysectomized zygote (43%). Increases in DNA, RNA, and protein in animals, arise when comparing normal animals with those that during CRG indicate that division and have lost weight after hypophysectomy, Such problems are overwere occurring in similar proportions. In the GH-deficient mouse, come by using the allometric relationship between KW and BW the amount Of DNA in renoprival kidneys was 0'451 mg (25). Another problem is the multiple hormone deficiency. Pituicompared with 0.439 mg in (NS). This suggests that tary ablation may obscure the effect that the hormones exert in replication was suppressed. The protein:DNA ratio increased from above and beyond their individual action, To avoid 20.91 to 24.27 (P < 0.001) and the RNA:DNA ratio increased from complications due to hypophysectomy, we chose, instead, to use 0.732 to 0.912 ( P < 0.001), suggesting that cell size was markedly a GH-deficient dwarf mouse strain, increased. These suggest that reduced amounts Of GH The Little mouse (lit), an atellotic dwarf strain (16), was used in may produce a dissociation between h~p'r~lasia and h~~e r t r o~h~, this study, ~h~ animal's autosomal recessive defect appears to be with CRG occurring predominantly by cellular hypertrophy. a selective deficiency of pituitary GH. resembling type 1 GH deficiency in humans (7, 16). The mice do not have neonatal or Speculation pubertal growth spurts, but the females are fertile. They grow with Growth hormone deficiency does not prevent compensatory repetitive pregnancies and can achieve normal weights after mulrenal growth, which occ...

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Section: Discussioncontrasting

confidence: 79%

Compensatory Renal Growth in the Mouse. II. The Effect of Growth Hormone Deficiency

et al. 1981

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“…The formula for SA closely resembles the allometric equation (32,39,41,44,45) but the power of 0.66 (for SA) is less than that found by allometry for KW (0.75-0.80) in most strains of rats. Recently Aschinberg et al (31) reported greater CRG in puppies compared with older dogs. However, their results are not directly comparable with ours because 75% rather than 50% renal ablation was used, and thus, the older dogs developed a degree of renal failure.…”

Section: Discussionmentioning

confidence: 96%

Compensatory Renal Growth in the Mouse. I. Allometric Approach to the Effect of Age

et al. 1981

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SummaryAllometry, defined as the relationship between the growth rates of organs to the weight of the whole body (38), was used to study the effect of age on the degree of compensatory renal growth (CRG) in the mouse. The normal growth of the kidneys relative to body weight (BW) was determined in animals between 5 to 50 days of age. In one group, nephrectomy and sham operations were performed at 5, 15, and 35 days of age. The remaining ("renoprival") kidney was removed 15 days postnephrectomy. In a second group, nephrectomy was performed on 5-day-old animals, the renoprival kidney being removed after 30 or 45 days. Regression equations were calculated by least-squares after logarithmic transformation and different groups were compared by analysis of covariance. The regression equation for the control kidney was kidney weight (KW) = 0.0093 BW0.86 (r = 0.96). The regression for renoprival kidneys in females was KW = 0.0142 BwO.'~ (r = O.%) after 15 days and, in comparison, was not significantly different after 30 to 45 days. The intrval between control and renoprival regressions was equivalent to a difference of 243% KW. In male mice, the regression for renoprival kidneys after 15 days was KW = 0.0103 BW0.96 (r = 0.98) and was not significantly different after 30 to 45 days. This study suggests that in young mice the time required for complete CRG may be a maximum of 15 days and that the amount of CRG does not depend on the age at operation. After CRG, a new equilibrium is reached which is therafter maintained up to a minimum of 50 days of age.

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“…Fig. 1 shows the loss of radioactivity in rRNA per milligram DNA During this 5-day period, the DNA content per kidney is unchanged (2). The degradation (Table 1).…”

Section: Degradation Rate Of Ribosomal Rnamentioning

confidence: 93%

“…Mice were maintained on Wayne chow and housed in quarters with a 12-h light, 12-h dark cycle. UNI and sham nephrectomy (sham) were performed as previously described (2,3). Isotope was administered by a subcutaneous injection of I mCi of (2.6 Ci/mmol, Schwarz/Mann Div., Becton, Dickinson & Co., Orangeburg, N.Y.).…”

Section: Methodsmentioning

confidence: 99%

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Ribosomal RNA metabolism during renal hypertrophy. Evidence of decreased degradation of newly synthesized ribosomal RNA.

Jm¹

1975

The Journal of Cell Biology

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Renal hypertrophy, induced by uninephrectomy (UNi), results in a 40 50% increase in the RNA content per cell 4 days postoperatively (1). Virtually no change in the DNA content occurs over the 4-day period (2). During the first 4 days of renal hypertrophy, incorporation of labeled nucleosides (uridine, orotate, and cytidine) into RNA showed no increase. Rates of synthesis of total kidney RNA from UNI mice, determined by measuring the labeling rates of RNA and UTP, showed no increase when compared to shams (3).Since the rate of synthesis is unchanged after UNI, the accretion of rRNA could occur by a decrease in degradation. However, the half-life of rRNA labeled before UNI is identical to sham value. Apparently RNA accretion during kidney hypertrophy does not occur by increased rate of transcription or decreased degradation of rRNA. These facts are compatible with the hypothesis that excess production and rapid degradation of newly synthesized rRNA occurs in the unstimulated kidney and that during renal hypertrophy the degradation is reduced. Other investigators have evidence of a similar phenomenon in the estrogenstimulated uterus (4), phytohemagglutininstimulated lymphocytes (5, 6), regenerating liver 260 THE JOURNAl. OF CH.t. Blot.oo ', 9 Vc, t.u~'u: 64. 1975 9 pages 260-265 on

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Accretion and turnover of RNA in the renoprival kidney

Cited by 62 publications

References 0 publications

Compensatory Renal Growth in the Mouse. II. The Effect of Growth Hormone Deficiency

Compensatory Renal Growth in the Mouse. II. The Effect of Growth Hormone Deficiency

Compensatory Renal Growth in the Mouse. I. Allometric Approach to the Effect of Age

Ribosomal RNA metabolism during renal hypertrophy. Evidence of decreased degradation of newly synthesized ribosomal RNA.

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