Accreditation of the GHEP-ISFG proficiency test: One step forward to assure and improve quality

Fernandez, Kenneth E.; Gómez, Josefina; Garcı́a-Hirschfeld, Julia; Cubillo, Eva; Torre, Carolina Sánchez de la; Vallejo, G.

doi:10.1016/j.fsigss.2015.09.204

Cited by 8 publications

(4 citation statements)

References 1 publication

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“…Numerous guidance papers on forensic STR typing and interpretation were published (e.g., [4][5][6][7][8][9][10]) that highlight the importance of internal and external quality control (QC). Community based (e.g., [11][12][13][14]) and commercial (see example in [15]) proficiency testing programs that are powerful in highlighting problems with the analytical procedure and interpretational issues of STR typing in a routine forensic laboratory have been implemented. However, published datasets that form the basis for statistical evaluation in forensic genetics are not yet part of any external standardized quality control program -despite a growing number of contributions.…”

Section: Status Quo Of Autosomal Str Quality Control and Databasingmentioning

confidence: 99%

Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on quality control of autosomal Short Tandem Repeat allele frequency databasing (STRidER)

Bodner

Bastisch

Butler

et al. 2016

Forensic Science International: Genetics

141

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Section: Status Quo Of Autosomal Str Quality Control and Databasingmentioning

confidence: 99%

Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on quality control of autosomal Short Tandem Repeat allele frequency databasing (STRidER)

Bodner

Bastisch

Butler

et al. 2016

Forensic Science International: Genetics

141

View full text Add to dashboard Cite

“…In this respect, collaborative exercises and proficiency tests among laboratories allow monitoring forensic expertise and grant valuable insight into the quality of typing and accuracy of results. Such trials predate the analysis of STR markers and are regularly organized with varying focus by diverse entities in the forensic field, such as CTS [ 54 ], EDNAP [ 55 ], ESWG-ISFG [ 46 ], GEDNAP [ 48 ], GeFi [ 28 ], GHEP-ISFG [ 56 ] and others. Typically, aliquots or identical replicates of a few samples or stains are sent to participating laboratories for analysis; results are reported back to the organizers and (blindly) evaluated, sometimes also considering raw data to investigate the origin of inconsistencies in more detail [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

The STRidER Report on Two Years of Quality Control of Autosomal STR Population Datasets

Bodner

Parson

2020

Genes

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STRidER, the STRs for Identity ENFSI Reference Database, is a curated, freely publicly available online allele frequency database, quality control (QC) and software platform for autosomal Short Tandem Repeats (STRs) developed under the endorsement of the International Society for Forensic Genetics. Continuous updates comprise additional STR loci and populations in the frequency database and many further STR-related aspects. One significant innovation is the autosomal STR data QC provided prior to publication of datasets. Such scrutiny was lacking previously, leaving QC to authors, reviewers and editors, which led to an unacceptably high error rate in scientific papers. The results from scrutinizing 184 STR datasets containing >177,000 individual genotypes submitted in the first two years of STRidER QC since 2017 revealed that about two-thirds of the STR datasets were either being withdrawn by the authors after initial feedback or rejected based on a conservative error rate. Almost no error-free submissions were received, which clearly shows that centralized QC and data curation are essential to maintain the high-quality standard required in forensic genetics. While many errors had minor impact on the resulting allele frequencies, multiple error categories were commonly found within single datasets. Several datasets contained serious flaws. We discuss the factors that caused the errors to draw the attention to redundant pitfalls and thus contribute to better quality of autosomal STR datasets and allele frequency reports.

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“…In brief, the exercise was organized in two steps. In a first phase, four samples from the annual GHEP-ISFG proficiency tests were used for quality control purposes (samples 1 to 4 included in 2012 or 2013 kinship basic modules; see [15] for details on GHEP-ISFG proficiency tests). Written informed consent was obtained from the donors for using these samples in the exercise.…”

Section: Ghep-isfg Working Commission On Autosomal Indels For Human Imentioning

confidence: 99%

A GHEP-ISFG collaborative study on the genetic variation of 38 autosomal indels for human identification in different continental populations

Pereira

Alves

Aler

et al. 2018

Forensic Science International: Genetics

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A collaborative effort was carried out by the Spanish and Portuguese Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) to promote knowledge exchange between associate laboratories interested in the implementation of indel-based methodologies and build allele frequency databases of 38 indels for forensic applications. These databases include populations from different countries that are relevant for identification and kinship investigations undertaken by the participating laboratories. Before compiling population data, participants were asked to type the 38 indels in blind samples from annual GHEP-ISFG proficiency tests, using an amplification protocol previously described. Only laboratories that reported correct results contributed with population data to this study. A total of 5839 samples were genotyped from 45 different populations from Africa, America, East Asia, Europe and Middle East. Population differentiation analysis showed significant differences between most populations studied from Africa and America, as well as between two Asian populations from China and East Timor. Low F values were detected among most European populations. Overall diversities and parameters of forensic efficiency were high in populations from all continents.

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Accreditation of the GHEP-ISFG proficiency test: One step forward to assure and improve quality

Cited by 8 publications

References 1 publication

Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on quality control of autosomal Short Tandem Repeat allele frequency databasing (STRidER)

Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on quality control of autosomal Short Tandem Repeat allele frequency databasing (STRidER)

The STRidER Report on Two Years of Quality Control of Autosomal STR Population Datasets

A GHEP-ISFG collaborative study on the genetic variation of 38 autosomal indels for human identification in different continental populations

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