Acclimatization of Thalassiosira pseudonana Photosynthetic Membranes to Environmental Temperature Changes

Bojko, Monika; Olchawa-Pajor, Monika; Chyc, Marek; Goss, Reimund; Schaller-Laudel, Susann; Latowski, Dariusz

doi:10.11159/icepr17.120

Cited by 5 publications

(7 citation statements)

References 37 publications

(45 reference statements)

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“…In contrast to cyanobacteria and green algae, the photosynthetic capacity, as well as the growth rate, of diatoms seems to be less sensitive to these rapid and pronounced changes (Litchman, ; Mitrovic, Howden, Bowling, & Buckney, ; Nicklisch, ). This resistance suggests an unusual flexibility of the diatom photosynthetic membranes (Bojko et al, , ; MacIntyre, Kana, & Geider, ; Schubert & Forster, ). It is commonly known that the fluidity of all kinds of cell membranes is strongly affected by changes of some environmental factors, especially temperature (Los & Murata, ).…”

Section: Introductionmentioning

confidence: 99%

Diadinoxanthin de‐epoxidation as important factor in the short‐term stabilization of diatom photosynthetic membranes exposed to different temperatures

Bojko

Olchawa-Pajor

Goss

et al. 2018

Plant Cell & Environment

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The importance of diadinoxanthin (Ddx) de‐epoxidation in the short‐term modulation of the temperature effect on photosynthetic membranes of the diatom Phaeodactylum tricornutum was demonstrated by electron paramagnetic resonance (EPR), Laurdan fluorescence spectroscopy, and high‐performance liquid chromatography. The 5‐SASL spin probe employed for the EPR measurements and Laurdan provided information about the membrane area close to the polar head groups of the membrane lipids, whereas with the 16‐SASL spin probe, the hydrophobic core, where the fatty acid residues are located, was probed. The obtained results indicate that Ddx de‐epoxidation induces a two component mechanism in the short‐term regulation of the membrane fluidity of diatom thylakoids during changing temperatures. One component has been termed the “dynamic effect” and the second the “stable effect” of Ddx de‐epoxidation. The “dynamic effect” includes changes of the membrane during the time course of de‐epoxidation whereas the “stable effect” is based on the rigidifying properties of Dtx. The combination of both effects results in a temporary increase of the rigidity of both peripheral and internal parts of the membrane whereas the persistent increase of the rigidity of the hydrophobic core of the membrane is solely based on the “stable effect.”

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Section: Introductionmentioning

confidence: 99%

Diadinoxanthin de‐epoxidation as important factor in the short‐term stabilization of diatom photosynthetic membranes exposed to different temperatures

Bojko

Olchawa-Pajor

Goss

et al. 2018

Plant Cell & Environment

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“…During the first two weeks of the diatom cultures growth at low and moderate temperatures (12 and 20°C), the decrease of the cell proliferation (measured as optical density, OD600), as well as the chlorophylls content with increasing of the protein concentration, were observed. We also observed the increase of polyunsaturated and decrease of saturated fatty acids content (analysed by gas chromatography, GC), which resulted in stabilisation of the thylakoid membrane fluidity (S parameter, analysed by EPR method) and photosynthetic activity (Fv/Fm) [7,8]. The results show that regulation of membrane fluidity by interaction of fatty acids, proteins and pigments is the most important factor in adaptation strategies of diatoms to environmental temperature changes (Tab.1).…”

Section: The First Face -Acclimation Mechanisms Of Diatoms To Environmentioning

confidence: 70%

“…We grew the diatoms P. tricornutum and T. pseudonana under specified conditions, analysed the fatty acid compounds (by static headspace gas chromatography with flame ionization detector, HSGC-FID) and thus confirmed the presence of DHA, dominant amount of EPA and very low omega-3/omega-6 FA ratio ( Fig. 4) [4,8,37,38]. The results suggest, that the marine diatoms could be considered as a potential source for industrial production of omega-3 LC-PUFAs.…”

Section: The Fourth Face -Treasury Bioactive Compounds In Diatomsmentioning

confidence: 89%

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The Five Different Faces of the Diatoms

Bojko¹,

Latowski²

2019

Proceedings of the 5th World Congress on New Technologies

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Diatoms are one of the most important groups of photosynthetic microorganisms ubiquitous in all aquatic ecosystems. These inconspicuous organisms have achieved spectacular ecological success and furthermore, are used as model organisms in scientific research in the field of biochemistry and genetics. Their ecological success is provided by their ability to tolerance and fast acclimation to rapid changing environmental conditions. Ecological significance and the ease of culture facilitate investigation and attempts of explanation the mechanisms of processes occurring in these organisms on the physiological, biochemical and genetic level. This review describes a number of our investigation strategies and techniques to understand the phenomenon of these organisms. We focused on five important aspects of diatoms biology, presented here as the five different faces of these algae. Acclimation mechanisms to environmental factors (temperature, light, heavy metals, age of the culture), on physiological and biochemical level at short and long time exposition of diatoms, are presented as the first diatoms face. The second face presents diatoms as organisms useful for phytoremediation. The results of the molecular analyses of the selected enzymes involved in photoprotective mechanisms of diatoms is the third face of these algae. The significance of diatoms as a treasury of bioactive compounds (xanthophylls and long chain polyunsaturated fatty acids) important in human diet, is displayed as the fourth face. Finally, as the only one which has not been investigated in our laboratory, we describe the fifth face of diatomsthe rich source of biofuels. In our research we used two model diatoms species: Phaeodactylum tricornutum and Thalassiosira pseudonana.

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“…The effect of temperature and adaptation mechanisms of marine diatoms P. tricornutum and T. pseudonana, growing two weeks in batch cultures, was presented and discussed in our previous papers [9,13]. In this document we present the effect of temperature and light intensity on P. tricornutum growth, cells size and selected photosynthesis parameters, which were analyzed during six months.…”

Section: Resultsmentioning

confidence: 97%

Acclimatization of Diatom Phaeodactylum Tricornutum to Long-Term Environmental Temperature and Light Intensity Changes

Warkiewicz¹,

Strączek²,

Latowski³

et al. 2018

Proceedings of the 4th World Congress on New Technologies

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Diatoms are the dominant primary producers in a number of oceanographic settings that require high-nutrient and turbulent conditions. In addition, diatoms are sensitive indicator of environmental changes and small modifications of environment result in measurable changes in physiological and biochemical parameters. In the present study the model diatom species, Phaeodactylum tricornutum, was culturing for 6 months and the influence of two environmental factors i.e. temperature and light intensity, as the model of greenhouse effect, was tested. Two temperatures i.e. 12°C (low) and 20°C (moderate), and two light intensities i.e. 6µ Em-2 s-1 (low) and 40 µEm-2 s-1 (moderate) were selected. Three main growth phases of diatom cultures were observed. In the first phase (first two weeks) the optical density at 600 nm (OD) of cultures as well as photosynthetic activity measured as Fv/Fm parameter, increased under all experimental conditions. However, in third growth phase i.e. after four months of experiments, the decrease of OD was detected only at a higher temperature. Values of Fv/Fm parameter showed that photosynthetic activity of P. tricornutum was more sensitive to light intensity than to growth temperature and lower photosynthetic activity was detected at moderate light intensity. In the third phase photosynthetic activity was also well correlated with chlorophyll a concentration and the carotenoid content. The obtained results indicated that the growth rate of diatom cultures depends on temperature whereas photosynthetic activity is more sensitive to light intensity. Low temperature as well as low light intensity were found to be better environmental conditions which ensure a sustainable and long-term growth of diatoms.

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Acclimatization of Thalassiosira pseudonana Photosynthetic Membranes to Environmental Temperature Changes

Cited by 5 publications

References 37 publications

Diadinoxanthin de‐epoxidation as important factor in the short‐term stabilization of diatom photosynthetic membranes exposed to different temperatures

Diadinoxanthin de‐epoxidation as important factor in the short‐term stabilization of diatom photosynthetic membranes exposed to different temperatures

The Five Different Faces of the Diatoms

Acclimatization of Diatom Phaeodactylum Tricornutum to Long-Term Environmental Temperature and Light Intensity Changes

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