Acclimation to the Growth Temperature and the High-Temperature Effects on Photosystem II and Plasma Membranes in a Mesophilic Cyanobacterium, Synechocystis sp. PCC6803

Inoue, Naoki; Taira, Yoshiko; Emi, Takashi; Yamane, Yoshihiro; Kashino, Yasuhiro; Koike, Hiroyuki; Satoh, Kazuhiko

doi:10.1093/pcp/pce147

Cited by 77 publications

(60 citation statements)

References 30 publications

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“…1A). The temperature optimum of Synechocystis is 30°C to 32°C, but it can grow for a few days at 43°C (Inoue et al, 2001;Tuominen et al, 2006;Gunnelius et al, 2010). In mild heat stress at 43°C, both the DsigB and DsigCDE strains grew as well as the control strain (Fig.…”

Section: Resultsmentioning

confidence: 95%

The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803

et al. 2011

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Changing of principal s factor in RNA polymerase holoenzyme to a group 2 s factor redirects transcription when cyanobacteria acclimate to suboptimal environmental conditions. The group 2 sigma factor SigB was found to be important for the growth of the cyanobacterium Synechocystis sp. PCC 6803 in high-salt (0.7 M NaCl) stress but not in mild heat stress at 43°C although the expression of the sigB gene was similarly highly, but only transiently up-regulated at both conditions. The SigB factor was found to regulate many salt acclimation processes. The amount of glucosylglycerol-phosphate synthase, a key enzyme in the production of the compatible solute glucosylglycerol, was lower in the inactivation strain DsigB than in the control strain. Addition of the compatible solute trehalose almost completely restored the growth of the DsigB strain at 0.7 M NaCl. High-salt conditions lowered the chlorophyll and phycobilin contents of the cells while protective carotenoid pigments, especially zeaxanthin and myxoxanthophyll, were up-regulated in the control strain. These carotenoids were up-regulated in the DsigCDE strain (SigB is the only functional group 2 s factor) and down-regulated in the DsigB strain under standard conditions. In addition, the HspA heat shock protein was less abundant and more abundant in the DsigB and DsigCDE strains, respectively, than in the control strain in high-salt conditions. Some cellular responses are common to heat and salt stresses, but pretreatment with mild heat did not protect cells against salt shock although protection against heat shock was evident.

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Section: Resultsmentioning

confidence: 95%

The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803

et al. 2011

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“…Actually, thermophilic cyanobacteria grow in hot springs and deserts, whereas mesophilic cyanobacteria can tolerate temperatures up to 50°C [16]. Heat shock response has been already studied in cyanobacteria both at the transcription/expression level of specific genes and proteins and at the transcriptomic/ proteomic level of the whole cell (see Ref.…”

Section: Proteomics Of the Heat Shock Responsementioning

confidence: 99%

Proteomic analyses of the response of cyanobacteria to different stress conditions

Castielli¹,

Cerda²,

Navarro³

et al. 2009

FEBS Letters

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a b s t r a c tCyanobacteria are significant contributors to global photosynthetic productivity, thus making it relevant to study how the different environmental stresses can alter their physiological activities. Here, we review the current research work on the response of cyanobacteria to different kinds of stress, mainly focusing on their response to metal stress as studied by using the modern proteomic tools. We also report a proteomic analysis of plastocyanin and cytochrome c 6 deletion mutants of the cyanobacterium Synechocystis sp. PCC 6803 grown under copper or iron deprivation, as compared to wild-type cells, so as to get a further understanding of the metal homeostasis in cyanobacteria and their response to changing environmental conditions.

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“…Oxygen evolution or consumption activities were measured by a Clark-type oxygen electrode (Rank Brothers) as described previously (Inoue et al, 2001). …”

Section: Measurement Of Oxygen Evolution or Consumption Activitiesmentioning

confidence: 99%

slr1923 of Synechocystis sp. PCC6803 Is Essential for Conversion of 3,8-Divinyl(proto)chlorophyll(ide) to 3-Monovinyl(proto)chlorophyll(ide)

et al. 2008

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The deduced amino acid sequence of an slr1923 gene of Synechocystis sp. PCC6803 is homologous to archaean F 420 H 2 dehydrogenase, which acts as a soluble subcomplex of reduced nicotinamide adenine dinucleotide dehydrogenase complex I. In this study, the gene was inactivated and characteristics of the mutant were analyzed. The mutant grew slower than the wild type under 100 mE m 22 s 21 but did not grow under high light intensity (300 mE m 22 s 21 ). The cellular content of chlorophyll was lower in the mutant, and the absorption spectrum showed a shift in the absorption peak of the Soret band to a longer wavelength by about 10 nm compared with the wild type. It was found, by high-performance liquid chromatography analysis, that the retention time of chlorophyll of the mutant is shorter than that of the wild type and that the peak wavelength of the Soret band was also shifted to a longer wavelength by 11 nm. Proton nuclear magnetic resonance analysis of the chlorophyll of the mutant revealed that the ethyl group of position 8 of ring B is replaced with a vinyl group. The spectrum indicates that the chlorophyll of the mutant is not a normal (3-vinyl)chlorophyll a but a 3,8-divinylchlorophyll a. These results strongly suggest that the Slr1923 protein is essential for the conversion from divinylchlorophyll(ide) to normal chlorophyll(ide). We thus designate this gene cvrA (a gene indispensable for cyanobacterial vinyl reductase).Land plants, algae, cyanobacteria, and photosynthetic bacteria use various types of chlorophyll molecules (chlorophyll [chl] a, b, c, and d and bacteriochlorophyll a, b, c, etc.) as inevitable photon-capturing pigments in photosynthesis. Extensive studies with various organisms by biochemical and genetic approaches have disclosed many aspects related to chlorophyll metabolism (for review, see Beale, 1993Beale, , 1999Senge and Smith, 1995). However, the functional or regulatory genes related to various chlorophyll biosynthetic pathways have not yet been fully elucidated. In land plants, chlorophyll precursors are sometimes accumulated as both 3-monovinyl (MV-) or 3,8-divinyl (DV-) intermediates, and the ratio between the two forms changes depending on the species, tissues, and growth conditions (Kim and Rebeiz, 1996). Chlorophyll biosynthetic heterogeneity is assumed to originate mainly in parallel DV-and MV-chl biosynthetic routes interconnected by 8-vinyl reductases (8 VRs) that convert DV-tetrapyrroles to MV-tetrapyrroles by conversion of the vinyl group at position 8 of ring B to the ethyl group. So far, five 8-VR activities have been detected at the levels of DV-Mg-protophorphyrin IX, Mg-protomonomethyl ester, protochlorophyllide (Pchlide) a, chlorophyllide (chlide) a, and chl a (Kolossov et al.,

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Acclimation to the Growth Temperature and the High-Temperature Effects on Photosystem II and Plasma Membranes in a Mesophilic Cyanobacterium, Synechocystis sp. PCC6803

Cited by 77 publications

References 30 publications

The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803

The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803

Proteomic analyses of the response of cyanobacteria to different stress conditions

slr1923 of Synechocystis sp. PCC6803 Is Essential for Conversion of 3,8-Divinyl(proto)chlorophyll(ide) to 3-Monovinyl(proto)chlorophyll(ide)

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