Acclimation to prolonged hypoxia alters hemoglobin isoform expression and increases hemoglobin oxygen affinity and aerobic performance in a marine fish

Pan, Yihang K.; Ern, Rasmus; Morrison, Phillip R.; Brauner, Colin J.; Esbaugh, Andrew J.

doi:10.1038/s41598-017-07696-6

Cited by 38 publications

(25 citation statements)

References 53 publications

(50 reference statements)

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“…Blue crab ( Callinectes sapidus ) and various other crustacean species have been shown to be tolerant of low oxygen conditions as they are capable of regulating oxygen transport 37 presumably due to induction of oxygen-transporting proteins. Fish have been shown to increase hemoglobin levels in response to low oxygen conditions 38 .…”

Section: Discussionmentioning

confidence: 99%

Hemoglobin Levels Modulate Nitrite Toxicity to Daphnia magna

Eytcheson

LeBlanc

2018

Sci Rep

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Nitrogenous compounds enter the environment through various anthropogenic sources. Among these are nitrate (NO3−) and nitrite (NO2−) which can oxidize the heme moiety of hemoglobin and reduce the oxygen-carrying capacity of the molecule resulting in toxicity. Of the two anions, nitrite is more toxic. Hemoglobin levels are influenced by environmental conditions; thus, we hypothesized that hemoglobin levels would influence the toxicity of nitrite with low hemoglobin levels resulting in enhanced toxicity and high hemoglobin levels resulting in reduced toxicity. We tested this hypothesis by elevating hemoglobin levels with pyriproxyfen treatment and lowering hemoglobin levels using siRNA in Daphnia magna. Exposure to pyriproxyfen significantly elevated hemoglobin mRNA levels and induced copper coloration of the organisms, indicative of increased hemoglobin protein accumulation. siRNA treatment significantly reduced hemoglobin mRNA levels in both untreated and pyriproxyfen-treated organisms and attenuated copper coloration. Pyriproxyfen treatment increased the tolerance of daphnids to the acute toxicity of nitrite approximately 2-fold while siRNA treatment significantly decreased the tolerance of daphnids to nitrite toxicity. Results indicate that increased hemoglobin levels increase the tolerance of daphnids to nitrite toxicity which may serve to protect daphnids in environments subject to hemoglobin-elevating hypoxia or elevated temperatures.

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Section: Discussionmentioning

confidence: 99%

Hemoglobin Levels Modulate Nitrite Toxicity to Daphnia magna

Eytcheson

LeBlanc

2018

Sci Rep

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“…A follow‐up review of hypoxia tolerance in various species of intertidal sculpins, revealed p crit values ranging 26–36 mmHg (Richards, ). p crit in the marine red drum, Sciaenops ocellatus (L. 1766) is 28–36 mmHg at 24°C (Pan et al, ). p crit Has been examined in the context of a fast‐slow lifestyle continuum in fishes, with values ranging from 19–38 mmHg (Stoffels, ).…”

Section: Discussionmentioning

confidence: 99%

Developmental changes in oxygen consumption and hypoxia tolerance in the heat and hypoxia‐adapted tabasco line of the Nile tilapia Oreochromis niloticus, with a survey of the metabolic literature for the genus Oreochromis

Burggren

Méndez-Sánchez

Martínez-Bautista

et al. 2019

Journal of Fish Biology

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The genus Oreochromis is among the most popular of the tilapiine cichlid tribe for aquaculture.However, their temperature and hypoxia tolerance, if tested at all, is usually tested at temperatures of 20-25 C, rather than at the considerably higher temperatures of 30-35 C typical of tropical aquaculture. We hypothesized that both larvae and adults of the heat and hypoxia-adapted Tabasco-line of the Nile tilapia Oreochromis niloticus would be relatively hypoxia-tolerant. Oxygen consumption rate ( _ MO 2 ), Q 10 and aquatic surface respiration (ASR) was measured using closed respirometry at 2 (c. 0.2 g), 30 (c. 2-5 g), 105 c. (10-15 g) and 240 (c. 250 g) days of development, at 25 C, 30 C and 35 C. _ MO 2 at 30 C was inversely related to body mass: c. 90 μM O 2 g −1 /h in larvae down to c. 1 μM O 2 g −1 /h in young adults. Q 10 for _ MO 2 was typical for fish over the range 25-35 C of 1.5-2.0. ASR was exhibited by 50% of the fish at pO 2 of 15-50 mmHg in a temperature-dependent fashion. However, the largest adults showed notable ASR only when pO 2 fell to below 10 mmHg. Remarkably, p crit for _ MO 2 was 12-17 mmHg at 25-30 C and still only 20-25 mmHg across development at 35 C. These values are among the lowest measuredfor teleost fishes. Noteworthy is that all fish maintain equilibrium, ventilated their gills and showed routine locomotor action for 10-20 min after _ MO 2 ceased at near anoxia and when then returned to oxygenated waters, all fish survived, further indicating a remarkable hypoxic tolerance. Remarkably, data assembled for _ MO 2 from >30 studies showed a > x2000 difference, which we attribute to calculation or conversion errors. Nonetheless, p crit was very low for allOreochromis sp. and lowest in the heat and hypoxia-adapted Tabasco line. K E Y W O R D Shypoxia tolerance, _ MO 2 , Oreochromis, oxygen consumption, Tabasco-line tilapiadevelopment

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“…6A and B, at its NOEC (0.8 µg/L), AEO-7 did not affect the level of hemoglobin in our zebra sh embryos, whereas at a concentration of 3.2 μg/L, the number of hemoglobin-positive cells was reduced. This reduction could re ect decreased production of red blood cells in the bone marrow and/or lowered hemoglobin synthesis in erythrocytes due to blockage of heme synthesis [66,67].…”

Section: At Its Noec Aeo-7 Does Not Cause Hematopoietic Toxicity In mentioning

confidence: 99%

AEO-7 Surfactant is “super Toxic” and Induces Severe Cardiac, Liver and Locomotion Damage in Zebrafish Embryos

Al-Asmakh

Majdalawieh

Abdullah

et al. 2020

Preprint

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BackgroundFatty alcohol polyoxyethylene ether-7 (AEO-7), a non-ionic surfactant, has recently been receiving extensive attention from the ocean pipeline industry for its ability to inhibit corrosion. However, the present lack of information concerning the potential environmental toxicity of AEO-7, especially towards aquatic organisms, is a major impediment to its wider application. Here, we assess potential adverse effects of AEO-7 on zebrafish embryos employing a variety of assays, including (i) a mortality/survival assay which allowed the median lethal concentration (LC50) to be calculated; (ii) a teratogenicity assay on the basis of which the no observed effect concentration (NOEC) was determined; and (iii) specific assays of cardiotoxicity, neurotoxicity (based on locomotion), hematopoietic toxicity (the level of hemoglobin as revealed by o-dianisidine staining) and hepatotoxicity (liver steatosis and yolk retention examined by staining with Oil Red O). ResultsAEO-7 caused mortality with a calculated LC50 of 15.35 μg/L, which, according to the U.S. Fish and Wildlife Service (USFWS) Acute Toxicity Rating scale, should be considered “super toxic”. Although at its NOEC (0.8 μg/L), there were no signs of significant teratogenicity, cardiotoxicity, or hemopoiesis toxicity, 3.2 µg/L AEO-7 exerted dramatic detrimental effects on organ development. ConclusionOn the basis of these findings, we recommend that the industrial usage and environmental impact of AEO-7 be re-evaluated and strictly monitored by environmental and public health organizations.

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Acclimation to prolonged hypoxia alters hemoglobin isoform expression and increases hemoglobin oxygen affinity and aerobic performance in a marine fish

Cited by 38 publications

References 53 publications

Hemoglobin Levels Modulate Nitrite Toxicity to Daphnia magna

Hemoglobin Levels Modulate Nitrite Toxicity to Daphnia magna

Developmental changes in oxygen consumption and hypoxia tolerance in the heat and hypoxia‐adapted tabasco line of the Nile tilapia Oreochromis niloticus, with a survey of the metabolic literature for the genus Oreochromis

AEO-7 Surfactant is “super Toxic” and Induces Severe Cardiac, Liver and Locomotion Damage in Zebrafish Embryos

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