Acclimation of tobacco plantlets to ex vitro conditions as affected by application of abscisic acid

Pospíšilová, Jana; Wilhelmová, N.; Synková, Helena; Čatský, J.; Krebs, D.; Tichá, Ingrid; Hanáčková, Barbora; Snopek, Jiří

doi:10.1093/jxb/49.322.863

Cited by 53 publications

(16 citation statements)

References 30 publications

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“…One month after transplantation, stomatal conductance decreased considerably and stomata responses to air humidity, irradiance and internal CO 2 concentration resembled those of naturally grown plants (Fila et al, 1998). Similarly in Nicotiana tabacum, stomatal conductance and stomatal transpiration rate decreased to values found in seedlings 3 weeks after transfer to ex vitro conditions, but the cuticular transpiration rate decreased more slowly (Pospíšilová et al, 1988(Pospíšilová et al, , 1998. Development of ability of stomata to regulate transpiration rate during acclimation period was found in many other species, e.g., Brassica oleracea (Grout and Aston, 1977), Leucaena leucocephala (Dhawan and Bhojwani, 1987), Prunus serotina (Drew et al, 1992), Solanum laciniatum (Conner and Conner, 1984) and Solanum tuberosum (Baroja, 1995), Lycopersicon esculentum (Bhatia and Asnwath, 2004) but acclimation was usually slower than in tobacco.…”

Section: Development Of Cuticle Epicuticular Waxes and Functional Smentioning

confidence: 98%

“…Chl a and Chl b contents usually increased after transplantation (Trillas et al, 1995, Rival et al, 1997Synková, 1997;Pospíšilová et al, 1998). This was observed in originally photoautotrophically grown Nicotiana tabacum plantlets, however, in originally photomixotrophically grown plantlets an abrupt decrease in Chl a and Chl b contents during the first week after transplantation followed by a slow increase was found (Kadlecek et al, 1998).…”

Section: Changes In Chlorophyll a And B Contents And Net Photo-synthementioning

confidence: 99%

“…Improved chloroplast ultrastructure was observes e.g., in Liquidambar styraciflua (Wettstein and Sommer, 1982). Also photochemical efficiency, which may be characterized by variable to maximum fluorescence ratio (F v /F m ), increased after transfer of Elaeis guineensis and Nicotiana tabacum plantlets to ex vitro conditions (Rival et al, 1997;Synková, 1997;Pospíšilová et al, 1998). P N in Solanum tuberosum, Spathiphyllum floribundum, Capsicum annuum and Rehmania glutinosa plants decreased in the first days after transplantation and increased thereafter (Baroja et al, 1995;Van Huylenbroeck and Debergh, 1996;Estrada-Luna et al, 2001;Seon et al, 2000).…”

Section: Changes In Chlorophyll a And B Contents And Net Photo-synthementioning

confidence: 99%

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Acclimation of Plantlets to Ex Vitro Conditions: Effects of Air Humidity, Irradiance, Co2 Concentration and Abscisic Acid (A Review)

Pospíšilová¹,

Synková²,

Haisel³

et al. 2007

Acta Hortic.

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Plantlets grown in vitro might be easily impaired by sudden changes in environmental conditions after ex vitro transfer. They usually need several weeks under shade and gradually decreasing air humidity to acclimate to the new conditions and to correct all abnormalities in their anatomy and physiology induced by special conditions of in vitro culture. For plant survival, the most important changes include development of cuticle, epicuticular waxes, and effective stomatal regulation of transpiration leading to stabilization of water status. For plant growth, changes in photosynthetic parameters (chlorophyll content, chloroplast ultrastructure, efficiency of photosystem 2, net photosynthetic rate) ensuring fully autotrophic growth with the rate corresponding to naturally grown plants are the most important. Acclimation can be speeded up by hardening of plantlets in vitro or after transplantation by decreasing the transpiration rate by antitranspirants including abscisic acid, or by increasing photosynthetic rate by elevated CO 2 concentration. INTRODUCTION Within the last four decades, plant micropropagation has developed from a laboratory curiosity to a real industry. Its use in horticulture, agriculture and forestry is currently expanding worldwide. Micropropagation of many species can be achieved through the establishment of explants, their initial growth in vitro being followed by transplanting into the greenhouse or field. During in vitro cultivation, plantlets grow under constant temperature, very high air humidity, low irradiance, very low air turbulence, variable and often insufficient CO 2 concentration, water potential dependent on medium composition, sugars as carbon source, growth regulators in nutrient medium, ethylene and other volatiles, etc. The conditions are very dependent on the vessel and closure types (e.g., Solárová et al., 1996). Acclimation to these conditions leads to formation of plantlets with morphology, anatomy and physiology different from naturally grown plants (for review see, e.g.,

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Section: Development Of Cuticle Epicuticular Waxes and Functional Smentioning

confidence: 98%

Section: Changes In Chlorophyll a And B Contents And Net Photo-synthementioning

confidence: 99%

Section: Changes In Chlorophyll a And B Contents And Net Photo-synthementioning

confidence: 99%

See 1 more Smart Citation

Acclimation of Plantlets to Ex Vitro Conditions: Effects of Air Humidity, Irradiance, Co2 Concentration and Abscisic Acid (A Review)

Pospíšilová¹,

Synková²,

Haisel³

et al. 2007

Acta Hortic.

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show abstract

“…Chlorophyll (Chl) a and Chl b contents usually increased after transplantation (Trillas et al 1995, Rival et al 1997, Synková 1997, Van Huylenbroeck et al 2000, Osório et al 2005 and also improvement of chloroplast ultrastructure was observed (Wettstein and Sommer 1982).…”

Section: Introductionmentioning

confidence: 99%

Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

Pospíšilová¹,

Synková²,

Haisel³

et al. 2009

Biologia plant.

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The aim of this research was to determine whether exogenous abscisic acid (ABA) applied immediately after ex vitro transfer of in vitro grown plants can improve their acclimatization. Tobacco (Nicotiana tabacum L.) plantlets were transferred into pots with Perlite initially moistened either by water or 50 μM ABA solution and they were grown under low (LI) or high (HI) irradiance of 150 and 700 μmol m -2 s -1 , respectively. Endogenous content of ABA in tobacco leaves increased considerably after ABA application and even more in plants grown under HI. Stomatal conductance, transpiration rate and net photosynthetic rate decreased considerably 1 d after ex vitro transfer and increased thereafter. The gas exchange parameters were further decreased by ABA application and so wilting of these plants was limited. Chlorophyll (a+b) and β-carotene contents were higher in ABA-treated plants, but the content of xanthophyll cycle pigments was not increased. However, the degree of xanthophyll cycle pigments deepoxidation was decreased what also suggested less stress in ABA-treated plants. No dramatic changes in most chlorophyll a fluorescence parameters after ex vitro transfer suggested that the plants did not suffer from restriction of electron transport or photosystem damage.

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“…During this stage plants have to adapt to the new environment of greenhouse or field. The plantlets usually need some weeks of acclimation in shade with the gradual lowering of air humidity (Pospíšilová et al 1998). Fully developed V. negundo plantlets with proper shoots and roots were removed from culture medium, transferred to Thermocol cups containing sterile Soilrite and acclimatized by adopting the standard procedure.…”

mentioning

confidence: 99%

Direct plant regeneration from encapsulated nodal segments of Vitex negundo

Ahmad¹,

Anis²

2010

Biologia plant.

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An efficient protocol for encapsulation of nodal segments of Vitex negundo L. has been developed for the production of non-embryogenic synthetic seeds. The encapsulations of nodal segments were significantly affected by the concentrations of sodium alginate and calcium chloride. A 3 % Na 2 -alginate with 100 mM CaCl 2 has been found to be optimum concentration for the production of uniform synthetic seed. For germination, the synseeds were cultured on Murashige and Skoog (MS) basal medium supplemented with kinetin (KIN) and α-naphthalene acetic acid (NAA) either singly or in various combinations. MS medium containing 2.5 µM KIN in combination with 1.0 µM NAA was found to be the optimum for maximum (92.6 ± 3.71 %) plantlet conversion frequency. Well developed regenerated plantlets were hardened, acclimatized and established in field, where they grew well without any detectable variation.

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Acclimation of tobacco plantlets to ex vitro conditions as affected by application of abscisic acid

Cited by 53 publications

References 30 publications

Acclimation of Plantlets to Ex Vitro Conditions: Effects of Air Humidity, Irradiance, Co2 Concentration and Abscisic Acid (A Review)

Acclimation of Plantlets to Ex Vitro Conditions: Effects of Air Humidity, Irradiance, Co2 Concentration and Abscisic Acid (A Review)

Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

Direct plant regeneration from encapsulated nodal segments of Vitex negundo

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