Acclimation of Chlamydomonas reinhardtii to Different Growth Irradiances

Bonente, Giulia; Pippa, Sara; Castellano, Stefania; Bassi, Roberto; Ballottari, Matteo

doi:10.1074/jbc.m111.304279

Cited by 183 publications

(193 citation statements)

References 80 publications

(104 reference statements)

Supporting

Mentioning

164

Contrasting

Order By: Relevance

“…The gene expression of different LHCs has been reported to be affected by light acclimation (Teramoto et al, 2002;Durnford et al, 2003;Yamano et al, 2008) and to be NAB1 regulated (Mussgnug et al, 2005). It has been observed that longterm high-light exposure of C. reinhardtii cells leads to a 50% decrease of Chl content (Neale and Melis, 1986;Bonente et al, 2012) and to changes in Chl-to-Car ratio (Niyogi et al, 1997a;Baroli et al, 2003;Bonente et al, 2012), suggesting reduction of the antenna size (Neale and Melis, 1986), although, in a more recent report , it was concluded that the antenna size is not modulated by light in this alga. Recently, a dependence of the antenna components on the carbon availability also was reported.…”

mentioning

confidence: 99%

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

et al. 2016

View full text Add to dashboard Cite

Photosynthetic organisms are exposed to drastic changes in light conditions, which can affect their photosynthetic efficiency and induce photodamage. To face these changes, they have developed a series of acclimation mechanisms. In this work, we have studied the acclimation strategies of Chlamydomonas reinhardtii, a model green alga that can grow using various carbon sources and is thus an excellent system in which to study photosynthesis. Like other photosynthetic algae, it has evolved inducible mechanisms to adapt to conditions where carbon supply is limiting. We have analyzed how the carbon availability influences the composition and organization of the photosynthetic apparatus and the capacity of the cells to acclimate to different light conditions. Using electron microscopy, biochemical, and fluorescence measurements, we show that differences in CO 2 availability not only have a strong effect on the induction of the carbon-concentrating mechanisms but also change the acclimation strategy of the cells to light. For example, while cells in limiting CO 2 maintain a large antenna even in high light and switch on energy-dissipative mechanisms, cells in high CO 2 reduce the amount of pigments per cell and the antenna size. Our results show the high plasticity of the photosynthetic apparatus of C. reinhardtii. This alga is able to use various photoacclimation strategies, and the choice of which to activate strongly depends on the carbon availability.

show abstract

mentioning

confidence: 99%

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In C. reinhardtii, Bonente et al (2012) showed that CEF is up-regulated under low or moderate light conditions, relative to high light. Importantly, mutants devoid of either NDH or pgr5 (Kono et al, 2014) were found to be lethal under fluctuating light conditions compared to wild-type, suggesting that both CEF pathways are critical under frequent dark to light transitions which should include cells cycling between light and dark zones in high density cultures.…”

Section: Cyclic Electron Flows (Cef) and The Role Of Proton Gradient mentioning

confidence: 99%

“…Under steady state light, Bonente et al (2012) reported high light acclimation of C. reinhardtii led to a nearly 3-fold reduction in chlorophyll compared to low light treated cells, but a concomitant accumulation of relatively high amounts of zeaxanthin and lutein involved in photoprotection. The authors found the average antenna size of both photosystem I and II was, in fact, not modulated by acclimation; rather, the regulation affected the PSI : PSII ratio.…”

Section: Photoacclimationmentioning

confidence: 99%

“…Importantly, deactivation of photosynthetic enzymes is thought to be mediated by oxidized thioredoxins, molecular oxygen and accumulation of oxidants (Schürmann andBuchanan, 2008, Dangoor et al, 2012). Thus, Nikkanen et al (2014) (Grobbelaar et al 1995;Grobbelaar et al 1996;Bonente et al 2012). The activation state of photosynthetic enzymes under fluctuating conditions, particularly Rubisco which is the rate-limiting step of photosynthesis, may therefore be a critical component of PBR modelling.…”

Section: Redox Regulation Of Photosynthetic Enzyme Activitymentioning

confidence: 99%

See 1 more Smart Citation

Photosynthesis of microalgae in outdoor mass cultures and modelling its effects on biomass productivity for fuels, feeds and chemicals

Yarnold¹

View full text Add to dashboard Cite

Practical applications of microalgae include uses as feedstocks for sustainable fuels, feeds, nutraceuticals, and chemicals; for the treatment of wastewater and industrial flue gas; and for use as recombinant protein expression systems. Improvements in biomass yields are required for commercially viable and sustainable algal technologies, particularly for low value commodities such as biofuels.Although several algae exhibit higher growth rates than terrestrial crops, with theoretical upper limits of 8-10% photon conversion efficiency (PCE), solar-illuminated outdoor microalgal production systems typically have a PCE far below this and even below those achieved under laboratory conditions. The most intractable limiting factor is poor light distribution through the mass culture, where photoinhibitory light at the surface and dark zones deeper in the culture reduce photosynthetic productivity. Moreover, microalgae have evolved a suite of photoacclimation and photoregulation mechanisms to cope with rapid (seconds to minutes) light fluxes in natural environments, yet these processes remain poorly understood in well-mixed photobioreactors.The aim of this thesis study was to combine an experimental and theoretical approach to: 1) investigate and understand the photosynthetic response of algae under mass culture conditions; 2) develop a mathematical model which could accurately predict light-tobiomass productivities under a broad range of design scenarios for outdoor production systems; and 3) identify key biological and design parameters to maximise biomass yields.In the first part of the study, a light-to-biomass model was developed that could be easily applied to rapidly assess biomass yields of different algal strains under a range of design scenarios. This model predicts temporal and spatial irradiance at the reactor surface and through the mass culture with inputs of local solar radiation data, local coordinates, system properties and the optical properties of the culture. Local growth rates are then coupled to local light intensities within the reactor based on a static Haldane growth model derived from empirical growth-irradiance curves. Growth rates are integrated over space and time to compute areal and volumetric productivities.Validation of the model was attempted by conducting batch harvest experiments in a laboratory scale photobioreactor matrix which simulated diurnal light cycles representative of three 'typical' days of solar radiation in Brisbane. The strains used were ii Chlamydomonas reinhardtii and its truncated light-harvesting antenna mutant, tla1, reported to possess improved optical properties. Initially, the model did not satisfactorily predict growth under batch cultivation for the three different incident light conditions, overestimating productivity under low light days and overestimating on high light days.Subsequent adjustment of the model to include photoacclimative changes in the optical properties of the cell and a re-fit of the growth parameter values provided a more accurate m...

show abstract

“…Microalgae have been reported to radically increase their NPQ capacity even at moderate light intensities (Bonente et al 2012, Cazzaniga et al 2014 2014), though in one study, it was shown that the total contribution of these screening pigments to total NPQ was limited (Masojídek et al 1999). We did not measure a relative change in the absorption spectrum after a transfer from high light to mass culture.…”

Section: Possible Explanations For the Discrepancy Between The Model mentioning

confidence: 99%

Antenna size reduction in microalgae mass culture

Mooij¹

View full text Add to dashboard Cite

Acclimation of Chlamydomonas reinhardtii to Different Growth Irradiances

Cited by 183 publications

References 80 publications

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

Carbon Supply and Photoacclimation Cross Talk in the Green Alga Chlamydomonas reinhardtii

Photosynthesis of microalgae in outdoor mass cultures and modelling its effects on biomass productivity for fuels, feeds and chemicals

Antenna size reduction in microalgae mass culture

Contact Info

Product

Resources

About