Accidental Use of Milk With an Increased Concentration of Aflatoxins Causes Significant DNA Damage in Hospital Workers Exposed to Ionizing Radiation

Mrdjanović, Jasminka; Sudji, Jan; Srdjenović, Branislava; Dojcinovic, Sanja; Bogdanović, Višnja; Jakovljević, Dea Karaba; Jurišić, Vladimir

doi:10.3389/fpubh.2020.00323

Cited by 7 publications

(22 citation statements)

References 22 publications

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“…The study populations in the literature included were as shown in Table 1, with pediatric patients who undergo cardiac catheterization [ 6 ], radiation workers [ 11 , 15 , 18 ], radiography scoliosis patients [ 17 ], interventional physicians [ 10 ], catheter ablation patients [ 12 ], radiotherapy patients [ 11 , 13 , 16 ] and arthritis patients who underwent radiation synovectomy [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

“…The most common analytical method used for analysis of the 8-OHdG level in the included studies was ELISA [ 10 , 11 , 13 , 16 - 18 ]. Some different methods were used for analysis of 8-OHdG, including competitive immunochromatography with ICR-001 [ 6 ], Gas Chromatography Mass Spectrometry (GC-MS) analysis, Liquid Chromatography–Mass Spectrometry (LC-MS/MS) [ 12 ] and High-Performance Liquid Chromatography (HPLC) [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

“…In studies that were focused on collecting samples from radiation workers, the sample collection ranged between collecting urine samples at the end of shift work [ 15 ] and collecting venous blood samples in the morning [ 10 ]. The other studies did not state the time of collecting, but were limited to referring to the type of sample in terms of it being one blood sample [ 11 ] or one urine sample [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

“…the chance of malignancy will not increase [ 14 ]. Regarding the positive relationship between medical ionizing radiation and 8-OHdG level, the 8-OHdG levels for radiation workers exposed to medical ionizing radiation were higher compared to those who are not working in ionizing radiation procedures [ 10 , 11 , 15 , 18 ]. Moreover, several studies found the patients who were exposed to ionizing radiation had higher 8-OHdG levels compared with healthy people who were not exposed to medical ionizing radiation [ 6 , 12 , 16 , 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, another study suggested that the nuclear medicine group also had the highest urine concentration of 8-OHdG among the other radiographers [ 15 ]. Unplanned intake of milk for radiation workers with excess aﬂatoxin concentrations could lead to greater value of identified DNA damage biomarkers [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

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8-Hydroxy-2-Deoxyguanosine as Oxidative DNA Damage Biomarker of Medical Ionizing Radiation: A Scoping Review

AbuArrah

Setianto²,

Faisal

et al. 2021

J Biomed Phys Eng

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Background: Recent studies reported the significant expansion using 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as a biomarker of oxidative Deoxyribonucleic Acid (DNA) damage among human populations exposed to medical ionizing radiation, but a generalized overview about this topic has not been conducted yet. Objective: This scoping review of published literature examined recent trends in utilizing 8-OHdG biomarker to measure oxidative DNA damage induced by medical ionizing radiation and possible factors that may influence the 8-OHdG level. Material and Methods: Literature search was conducted in PubMed, Scopus and ProQuest databases for publications from 1984 to 2/12/2020. Included articles were: cohort studies, case-control studies, and cross–sectional studies, randomized and nonrandomized controlled trials. Excluded articles were: editorials, letters, personal opinions, newspaper articles, study plans, protocols, qualitative studies, case reports and series, in-vivo and vitro studies, animal research studies, reviews and meta-analyses. Results: According to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, we screened 141 articles, and 10 eligible studies met our inclusion criteria. All studies measured 8-OHdG as an oxidative DNA damage biomarker. The study results were contradictory concerning the relationship between the radiation dose and 8-OhdG level. 8-OHdG was mostly measured by enzyme-linked immunosorbent assay (ELISA) using urine samples. Sample size varied between (n=25-230) and included patients who underwent medical radiation procedures or workers exposed to ionizing radiation during their jobs. Conclusion: This scoping review findings showed 8-OHdG can be used as a promising biomarker to detect oxidative damage, resulting from medical ionizing radiation exposure despite external factors that may influence 8-OHdG levels.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

8-Hydroxy-2-Deoxyguanosine as Oxidative DNA Damage Biomarker of Medical Ionizing Radiation: A Scoping Review

AbuArrah

Setianto²,

Faisal

et al. 2021

J Biomed Phys Eng

View full text Add to dashboard Cite

show abstract

Hematological parameters and X-ray exposure among medical radiation workers: a systematic review and meta-analysis

Vaziri

Mirzaei

Saba

et al. 2022

Expert Review of Hematology

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Amelioration of Radiation-Induced Cell Death in Neuro2a Cells by Neutralizing Oxidative Stress and Reducing Mitochondrial Dysfunction Using N-Acetyl-L-Tryptophan

Kumar

Kumari

Pandey

et al. 2022

Oxidative Medicine and Cellular Longevity

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The deleterious effects of ionizing radiation on the central nervous system (CNS) are poorly understood. Radiation exposure during an accidental nuclear explosion, nuclear war, or radiotherapy causes severe brain damage. As a result, the current work is carried out to assess the radioprotective potential of N-acetyl-L-tryptophan (L-NAT) in neuronal cells. Radiation-induced cell death and its amelioration by L-NAT pretreatment were investigated using MTT, SRB, CFU, and comet assays. Flow cytometric and microscopic fluorescence assays were used to investigate radiation-induced oxidative stress, alteration in mitochondrial redox, Ca2+ homeostasis, depolarization of mitochondrial membrane potential, and its prevention with L-NAT pretreatment. Western blot analysis of Caspase-3, γ-H2aX, p53, ERK-1/2, and p-ERK-1/2 expression was carried out to identify the effects of L-NAT pretreatment on radiation-induced apoptosis and its regulatory proteins expression. The study demonstrated (MTT, SRB, and CFU assay) significant (~80%; p <0.001%) radioprotection in irradiated (LD50 IR dose) Neuro2a cells that were pretreated with L-NAT. In comparison to irradiated cells, L-NAT pretreatment resulted in significant (p <0.001%) DNA protection. A subsequent study revealed that L-NAT pretreatment of irradiated Neuro2a cells establishes oxidative stress by increasing antioxidant enzymes and mitochondrial redox homeostasis by inhibiting Ca2+ migration from the cytoplasm to the mitochondrial matrix and thus protects the mitochondrial membrane hyperpolarization. Caspase-3 and γ-H2aX protein expression decreased, while p-ERK1/2 and p53 expression increased in L-NAT pretreated irradiated cells compared to irradiated cells. Hence, L-NAT could be a potential radioprotective that may inhibit oxidative stress and DNA damage and maintain mitochondrial health and Ca2+ levels by activating p-ERK1/2 and p53 expression in Neuronal cells.

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Accidental Use of Milk With an Increased Concentration of Aflatoxins Causes Significant DNA Damage in Hospital Workers Exposed to Ionizing Radiation

Cited by 7 publications

References 22 publications

8-Hydroxy-2-Deoxyguanosine as Oxidative DNA Damage Biomarker of Medical Ionizing Radiation: A Scoping Review

8-Hydroxy-2-Deoxyguanosine as Oxidative DNA Damage Biomarker of Medical Ionizing Radiation: A Scoping Review

Hematological parameters and X-ray exposure among medical radiation workers: a systematic review and meta-analysis

Amelioration of Radiation-Induced Cell Death in Neuro2a Cells by Neutralizing Oxidative Stress and Reducing Mitochondrial Dysfunction Using N-Acetyl-L-Tryptophan

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