Accessory cells in murine Peyer's patch. I. Identification and enrichment of a functional dendritic cell.

Abstract

Previous studies have suggested that in vitro and in vivo immune responses are defective in Peyer's patch (PP) as a result of a deficiency in accessory cell number or function. However, we report here that enzymatic dissociation of PP does release a cell population with accessory activity in oxidative mitogenesis, i.e., the proliferation of periodate-modified T cells. The accessory activity present in PP is quantitatively similar to that of spleen. Accessory function is mediated by a cell type(s) that has the following characteristics: low buoyant density, lack of adherence to plastic or glass surfaces, lack of Fc receptors, and presence of surface Ia and the 33D1 dendritic cell (DC)-specific determinants. This PP accessory cell was markedly enriched by a novel technique. PP cells formed large aggregates when cultured for 16 h with irradiated, periodate-treated spleen cells. From the clusters we obtained a low density cell population that was 60% Ia positive, 33D1 positive, non-T and non-B, Fc receptor-negative, and dendritic in morphology. The DC-enriched populations were 60-80-fold enriched in accessory function relative to unfractionated PP. We can now compare PP accessory cells with accessory cells from other organs, and try to determine how PP dendritic cells contribute to the unique functions of this lymphoid organ.

Comparison of the Accessory Activity of Murine Peritoneal Cavity Macrophage Derived Dendritic Cells and Peritoneal Cavity Macrophages in a Mixed Lymphocyte Reaction.

“…In these and several other studies DC were reproducibly the most potent stimulators of allogeneic MLR when compared to macrophages. The results of this study are in agreement with those of Spalding et al [34], who demonstrated the stimulatory ability of mouse splenic DC in allogeneic MLR. Similar work on DC from mouse intestinal lamina propria and pig Peyer's patches [21,20,27,40], as well as studies of DC from the human colonic lamina propria demonstrated the presence of cells with potent stimulatory activity in the allogeneic MLR.…”

“…Professional APC are characterized by their constitutive high levels of major histocompartibility complex (MHC) class II expression, poor phagocytic ability, lack of sIg and Fc receptors, weak adherence to solid supports [34] and their ability to stimulate primed T cell proliferation in an antigen specific manner [29]. Studies have clearly shown that peritoneal macrophages are highly proliferating progenitors for functional DC [24,28].…”

A Bone Marrow-Derived APC in the Gut-Associated Lymphoid Tissue Captures Oral Antigens and Presents Them to Both CD4+ and CD8+ T Cells

“…These include different types of conventional APCs like dendritic cells (17)(18)(19)(20)(21)(22)(23)(24)(25), macrophages (26,27), and B cells (28) along with other putative APCs such as intestinal epithelial cells (29). We have found that the APC responsible for the presentation of oral OVA to both CD4 ϩ and CD8 ϩ T cells in the GALT is of bone marrow origin because T cell proliferation was evident only in chimeric mice in which the bone marrow-derived compartment expressed the correct MHC haplotype (Figs.…”

Dendritic Cells: A Specialized Complex System of Antigen Presenting Cells.

“…The current accepted concept is that DC comprise three distinct subpopulations, including two within the myeloid lineage (LC and interstitial DC) and one within the lymphoid lineage [107]. DC can be classified on the basis of their tissue location as: interdigitating reticulum cells in the dome and inter follicular areas (IFA) of lymphoid organs [59,168]; Veiled cells (non lymphoid DC) in afferent lymph [61,138]; Blood DC when in circulation [136,166]; Langerhans cells in the epidermis [150]; dermal DC when found in the dermis of the skin [142,146]; follicular DC, in B cell areas (follicles) of the spleen, lymph nodes and Peyer's patches [128]; and interstitial DC, in the interstitial connective tissues of non-lymphoid organs [45]. Tissues from which DC have been isolated are summarized in Table 1.…”