Accessing a Long‐Fluorescence‐Lifetime BODIPY Dye via Efficient Förster Resonance Energy Transfer Induced by Host‐Guest Doping

Abstract

The development of pure organic room temperature phosphorescence (RTP) luminophores with high quantum yield and long emission lifetime has attracted considerable attention due to their extensive optoelectronic and biomedical applications. The commonly used BODIPY dyes have strong UV‐Vis absorption, a relatively sharp emission peak, high quantum yield, and a short emission lifetime. By expending the molecular doping approach, we herein successfully achieved a long‐fluorescence‐lifetime BODIPY dye via efficient Förster resonance energy transfer (FRET) from RTP molecule o‐AI‐Cz to the short‐lived fluorescence emitter Bodipy‐2I. This host‐guest material possessed an ultralong lifetime of up to 595 ms and a long emission wavelength under ambient conditions, which has great potential in data encryption and anti‐counterfeiting applications. Moreover, this easily prepared FRET system exhibited an efficient response to two‐photon excitation in living cell fluorescence imaging.

Click-ready Iridium(III) complexes as versatile bioimaging probes for bioorthogonal metabolic labeling

“…17 If many efforts are constantly being made to improve bioorthogonal monosaccharide reporters and reactions in terms of specificity toward a given metabolic path, cell entry, level of incorporation, reporter group innocuity, stability or kinetics, [18][19][20][21][22][23][24] the most promising advances also stem from the development of improved click-ready molecular probes. For example, near-IR dyes, 25 long lifetime dyes, 26 dyes that rely on Forster's resonance energy transfer (FRET) interactions or quenching 27 or fluorogenic dyes 28 can help circumventing autofluorescence and non-specific background issues. In the search for new emissive probes to improve versatility, specificity and resolution in bioimaging studies, luminescent d 6 transition metal complexes such as iridium(III)-polypyridines are very attractive candidates.…”