Access to Phylogeny from Voltammetric Fingerprints of Seeds: the Asparagus Case

Abstract

1IntroductionIntersection of electrochemistryw ith vegetal matterh as av ariety of aspects.A part from the electrochemical determinationo fb ioactivec ompounds [1],c ontaminants [2,3] and antioxidant capacities [4][5][6][7],r ecognizing growth conditions and type of plants [8] and varietal discrimination of fruits [9] and leaves [10] have been reported. Conversely,v egetal matter can be used for electrode modification [11],e lectrochemical sensing [12,13] and as as upport for electrode modification [ 14,15].Electrochemical methods have as ignificant place in biological and environmentala nalyses. Much of electroanalytical methodsa re devotedt ot he determination of selected natural products in biological samples.T hese approaches,b ased on solution-phase electrochemistry, involve different types of modifiede lectrodes in order to achieve the levelso fs ensitivity and selectivity demanded by the individual analytest ob ed etermined in the presence of complex biological matrices [16][17][18][19].Itispossible, however, aiming av ariety of analytical targets which are based on the recordo ft he global electrochemicalr esponse of biological systems without determination of individual analytes [20,21].I nt his context, we have recently described the application of the voltammetry of immobilized particles (VIMP),asolid state electrochemical technique developed by Scholz et al. whichp rovidesa nalytical data on sparingly soluble solids [22][23][24],f or acquiring phylogenetici nformationb ased on the record of the "electrochemical fingerprint"d ue to the electroactive compounds existing in plantl eaves [20][21].H ere,w e presenta ne xpansiono ft his electrochemolomic approach focused on two objectives:i )r ecognition of the presence of characteristic voltammetric fingerprints at different taxonomic levels (family,g enus,s pecies), and ii) testing the possibility of discriminating different genetic linesw ithin different species of the same genus,i nb oth cases using the voltammetric response of seed extracts. Them ain objective was to test the hypothesis that the chemical composition of seed extracts should be less sensitive to local environmental factors and hence much more representative of geneticd ifferences,t han that of leaf extractsp reviously studied [20,21],i no rder to apply electrochemical data in phylogenetic studies.Molecularp hylogenyp lays an essential role in contemporary botany.P hylogenetic trees,t raditionally basedo n morphological/functional features,a re mainly derived fromg enetica nalysis mostly based in the identificationo f nucleotide sequences of nuclear and chloroplast regions [25].I ns everalc ases,c himeric nucleotide sequences are obtained so that discrepancies between molecular and morphological/functional classifications appear [26][27][28][29].Abstract:Am ethodology for characterizing vegetal taxonomic groupsf rom the voltammetric fingerprintso fp olyphenolic components of seeds is described. It is based on recording the voltammetric response of microparticulate films depositedo ng lassy...

Voltammetric Electrochemical Sensor for Phylogenetic Study in Acer Linn.

“…This work tried to statistically analyze all species using principal component analysis (PCA). PCA analysis is frequently used in phytochemical fingerprinting [14,18]. The electrochemical processes involve mainly solid-state reactions in the previous reports, where electron ingress is coupled, by reasons of charge conservation, to the entrance of protons on the solid lattice [18].…”

“…Electrochemical fingerprinting is a technique for collecting electrochemically active substances in plant tissues. Since the electrochemical signal is proportional to the type and content of the substance, it can reflect the difference of electrochemically active substance profile in plants [11][12][13][14][15][16][17][18][19][20][21][22][23]. For example, Doménech-Carbó et al [14] demonstrated the voltammetric fingerprints of Asparagus seeds can reflect the profiles of polyphenolic compounds (daidzein, ellagic acid, gallic acid, genistein, morin, quercetin, and rutin).…”

“…Since the electrochemical signal is proportional to the type and content of the substance, it can reflect the difference of electrochemically active substance profile in plants [11][12][13][14][15][16][17][18][19][20][21][22][23]. For example, Doménech-Carbó et al [14] demonstrated the voltammetric fingerprints of Asparagus seeds can reflect the profiles of polyphenolic compounds (daidzein, ellagic acid, gallic acid, genistein, morin, quercetin, and rutin). Ren et al [20] reported the electrochemical signal changes can be used for profiling hydrogen peroxide, nitric oxide, and pH in plants.…”

Antioxidative Properties of Selected Polish Honeys

“…However, natural methods for studying the redox reactions seem to be electroanalytical ones (Głód, Kiersztyn, & Piszcz, 2014). Indeed, TAP has been already estimated through potentiometry or voltammetry as analytical techniques (Hoyos-Arbeláez, Vázquez, & Contreras-Calderón, 2017;Doménech-Carbó et al, 2017;Głód, Haddad, & Alexander, 1992;Ortiz-Miranda et al, 2016;Doménech-Carbó et al, 2015;Sužnjević, Pastor, & Gorjanović, 2015). The righteous TAP measure is a standard redox potential, which is directly correlated with the half-wave potential in polarography and the voltammetric peak potential.…”

Electrochemical monitoring of ROS influence on seedlings and germination response to salinity stress of three species of the tribe Inuleae

“…23 This methodology exploits the possibility of electrochemical generation of ROS by applying reductive potential inputs to air-saturated aqueous solutions, [24][25][26] combined with the voltammetry of immobilized particles (VIMP), a solid state technique developed by Scholz et al 27,28 which provides analytical information on sparingly soluble solids attached to inert electrodes in contact with suitable electrolytes. 29 The VIMP was previously applied to determine antioxidant capabilities of vegetal products 30,31 and for electro-chemotaxonomic purposes 32,33 and monitoring plant defense against external stressors. 34 The current work reports the application of this methodology to monitor the interaction of ROS with the components of extracts from seedlings of Inula helenium L., Dittrichia viscosa (L.) Greuter, and Limbarda crithmoides (L.) Dumort, three species of the tribe Inuleae.…”