ACCERBATIN, a small molecule at the intersection of auxin and reactive oxygen species homeostasis with herbicidal properties

Hu, Yuming; Depaepe, Thomas; Smet, Dajo; Hoyerová, Klára; Klíma, Petr; Cuypers, Ann; Cutler, Sean R.; Buyst, Dieter; Morreel, Kris; Boerjan, Wout; Martins, José; Petrášek, Jan; Vandenbussche, Filip; Straeten, Dominique Van Der

doi:10.1093/jxb/erx242

Cited by 6 publications

(3 citation statements)

References 122 publications

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“…In addition, root length was measured in ImageJ. The triple response assay was carried out, as described previously (Hu et al, 2017), to evaluate the growth of etiolated seedlings. The length of 4-day-old hypocotyls and roots were measured in ImageJ.…”

Section: Methodsmentioning

confidence: 99%

The Ethylene Precursor ACC Affects Early Vegetative Development Independently of Ethylene Signaling

Vanderstraeten¹,

Depaepe²,

Bertrand³

et al. 2019

Front. Plant Sci.

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The plant hormone ethylene plays a pivotal role in virtually every aspect of plant development, including vegetative growth, fruit ripening, senescence, and abscission. Moreover, it acts as a primary defense signal during plant stress. Being a volatile, its immediate biosynthetic precursor, 1-aminocyclopropane-1-carboxylic acid, ACC, is generally employed as a tool to provoke ethylene responses. However, several reports propose a role for ACC in parallel or independently of ethylene signaling. In this study, pharmacological experiments with ethylene biosynthesis and signaling inhibitors, 2-aminoisobutyric acid and 1-methylcyclopropene, as well as mutant analyses demonstrate ACC-specific but ethylene-independent growth responses in both dark- and light-grown Arabidopsis seedlings. Detection of ethylene emanation in ethylene-deficient seedlings by means of laser-based photoacoustic spectroscopy further supports a signaling role for ACC. In view of these results, future studies employing ACC as a proxy for ethylene should consider ethylene-independent effects as well. The use of multiple knockout lines of ethylene biosynthesis genes will aid in the elucidation of the physiological roles of ACC as a signaling molecule in addition to its function as an ethylene precursor.

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Section: Methodsmentioning

confidence: 99%

The Ethylene Precursor ACC Affects Early Vegetative Development Independently of Ethylene Signaling

Vanderstraeten¹,

Depaepe²,

Bertrand³

et al. 2019

Front. Plant Sci.

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“…Transgenic calli were stained with NBT for superoxide activity analysis and with DAB for H 2 O 2 concentration detection in accordance with the methods of Hu et al . 56 . For NBT staining, 2 mM NBT (Sigma, Germany) solution was prepared in 20 mM phosphate buffer (pH 7.0).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome analysis provides insights into the stress response crosstalk in apple (Malus × domestica) subjected to drought, cold and high salinity

Zhou

et al. 2019

Sci Rep

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Drought, cold, and high salinity are three major abiotic stresses effecting apple tree growth and fruit production. Understanding the genetic mechanisms of crosstalk between stress responses signalling networks and identifying the genes involved in apple has potential importance for crop improvement and breeding strategies. Here, the transcriptome profiling analysis of in vitro -grown apple plants subjected to drought, cold and high salinity stress, showed a total of 377 upregulated and 211 downregulated common differentially expressed genes (DEGs) to all 3 stress treatments compared with the control. Gene Ontology (GO) analysis indicated that these common DEGs were enriched in ‘metabolic process’ under the ‘biological process’ category, as well as in ‘binding’ and ‘catalytic activity’ under the ‘molecular function’ category. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that common DEGs were mainly belong to the ‘biological functions’ category and 17 DEGs were identified in ‘environmental information processing’ sub-category which may act as signal transduction components in response crosstalk regulation. Overexpression of 5 upregulated genes individually, out of these 17 common DEGs in apple calli promoted the consistent upregulation of DREB6 , CBF1 and ZAT10 and increased the mass weight and antioxidase ability, implying these five common DEGs involved in multiple pathways and improved comprehensive resistance to stress.

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“…The limit of detection was in the range of 0.8 -8.0 fmol on the column, whereas the limit of quantification was in the range of 2 -20 fmol on column depending on the phytohormone. Ethylene emanation was measured by laser photo-acoustics [98].…”

Section: Phytohormone Bioassay and Measurementsmentioning

confidence: 99%

Insights into the Evolution of Multicellularity from the Sea Lettuce Genome

et al. 2018

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We report here the 98.5 Mbp haploid genome (12,924 protein coding genes) of Ulva mutabilis, a ubiquitous and iconic representative of the Ulvophyceae or green seaweeds. Ulva's rapid and abundant growth makes it a key contributor to coastal biogeochemical cycles; its role in marine sulfur cycles is particularly important because it produces high levels of dimethylsulfoniopropionate (DMSP), the main precursor of volatile dimethyl sulfide (DMS). Rapid growth makes Ulva attractive biomass feedstock but also increasingly a driver of nuisance "green tides." Ulvophytes are key to understanding the evolution of multicellularity in the green lineage, and Ulva morphogenesis is dependent on bacterial signals, making it an important species with which to study cross-kingdom communication. Our sequenced genome informs these aspects of ulvophyte cell biology, physiology, and ecology. Gene family expansions associated with multicellularity are distinct from those of freshwater algae. Candidate genes, including some that arose following horizontal gene transfer from chromalveolates, are present for the transport and metabolism of DMSP. The Ulva genome offers, therefore, new opportunities to understand coastal and marine ecosystems and the fundamental evolution of the green lineage.

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ACCERBATIN, a small molecule at the intersection of auxin and reactive oxygen species homeostasis with herbicidal properties

Abstract: ACCERBATIN is an ethylene-mimicking small molecule that affects auxin homeostasis and ROS accumulation in etiolated seedlings. In light-grown plants, it exhibits auxin-like herbicidal properties.

Cited by 6 publications

References 122 publications

The Ethylene Precursor ACC Affects Early Vegetative Development Independently of Ethylene Signaling

The Ethylene Precursor ACC Affects Early Vegetative Development Independently of Ethylene Signaling

Transcriptome analysis provides insights into the stress response crosstalk in apple (Malus × domestica) subjected to drought, cold and high salinity

Insights into the Evolution of Multicellularity from the Sea Lettuce Genome

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