Acceptance of Allogeneic Fibroblasts in Skin Equivalent Transplants

Sher, Stephanie E.; Hull, Barbara E.; Rosen, Seymour; Church, Diane; Friedman, Loren; Bell, Eugene

doi:10.1097/00007890-198311000-00015

Cited by 73 publications

(34 citation statements)

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“…On the other hand, experiments in rats have shown that transplanted fibroblasts will persist for over 13 months (30). When compared to the 20-to 30-day half-life of ADA in transfused erythrocytes (4), continued production of ADA in fibroblasts might allow smaller doses of genetically modified cells.…”

Section: Resultsmentioning

confidence: 99%

“…In addition to subcutaneous injection, genetically modified fibroblasts could be reintroduced into patients as part of a full-thickness skin-equivalent structure, an artificial skin of cultured fibroblast and epidermal cells that is quickly vascularized when transplanted onto freshly prepared graft beds (30,34). Of importance in deciding which route of introduction to use will be accessibility of the fibroblasts to circulating plasma.…”

Section: Resultsmentioning

confidence: 99%

“…Human diploid fibroblast (HDF) lines were isolated from skin biopsies of normal human donors using standard methods (17) and were maintained in culture for [22][23][24][25][26][27][28][29][30][31] (19). LHL, LHL2, and LSHL contain the hygromycin phosphotransferase gene (hph) from pLG90 (20).…”

Section: Methodsmentioning

confidence: 99%

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Efficient retrovirus-mediated transfer and expression of a human adenosine deaminase gene in diploid skin fibroblasts from an adenosine deaminase-deficient human.

Palmer¹,

Hock²,

Osborne³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

134

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Skin fibroblasts might be considered suitable recipients for therapeutic genes to cure several human genetic diseases; however, these cells are resistant to gene transfer by most methods. We have studied the ability of retroviral vectors to transfer genes into normal human diploid skin fibroblasts. Retroviruses carrying genes for neomycin or hygromycin B resistance conferred drug resistance to greater than 50% of the human fibroblasts after a single exposure to virus-containing medium. This represents at least a 500-fold increase in efficiency over other methods. Transfer was achieved in the absence of helper virus by using amphotropic retroviruspackaging cells. A retrovirus vector containing a human adenosine deaminase (ADA) cDNA was constructed and used to infect ADA-fibroblasts from a patient with ADA deficiency. The infected cells produced 12-fold more ADA enzyme than fibroblasts from normal individuals and were able to rapidly metabolize exogenous deoxyadenosine and adenosine, metabolites that accumulate in plasma in ADA-deficient patients and are responsible for the severe combined immunodeficiency in these patients. These experiments indicate the potential of retrovirus-mediated gene transfer into human fibroblasts for gene therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Efficient retrovirus-mediated transfer and expression of a human adenosine deaminase gene in diploid skin fibroblasts from an adenosine deaminase-deficient human.

Palmer¹,

Hock²,

Osborne³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

134

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“…12 The connective tissue fibroblast is an example of a type of cell that appears to be tolerated by hosts that are not immunologically matched with donors. 10,22 This apparent low immunogenicity has formed the basis for development of tissue engineering in which allogeneic fibroblasts from neonatal foreskins are used to produce an artificial dermis. 10,23,24 Growth factors decrease in burn wounds as a result of high levels of bacteria, which can impede the healing process.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Application of Allogeneic Fibroblast and Autologous Mesh Grafting With the Conventional Method in the Treatment of Third-Degree Burns

Moravvej

Hormozi

Hosseini

et al. 2016

Journal of Burn Care & Research

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Wound healing is a multipart process involving different cell types and growth factors. Third-degree burns are usually treated by early excision and skin grafting. Tissue engineering has been developed in this field in response to limitations associated with autografts. Allogeneic fibroblasts on meshed split thickness skin grafts (STSGs) are known to have useful properties in wound healing and can be used to construct a new model of living skin substitute. Fourteen patients were chosen from June 2009 until December 2010 as the sample for this study. After debridement and wound excision, meshed STSG was used to cover the entire wound. Alloskin (allofibroblasts cultured on a combination of silicone and glycosaminoglycan) was applied on one side and petroleum jelly-impregnated gauze (Iran Polymer and Petrochemical Institute) was applied on the other. The healing time, scar formation, and pigmentation score were assessed for the patients. All analyses were undertaken with SPSS 17 software. Alloskin demonstrated good properties compared to petroleum jelly-impregnated gauze. The average healing time and hypertrophic scar formation were significantly different between the two groups. In addition, the skin pigmentation score in the alloskin group was closer to normal. Alloskin grafting, including fibroblasts on meshed STSG, may be a useful method to reduce healing time and scar size and may require less autologous STSG in extensive burns where a high percentage of skin is burned and there is a lack of available donor sites.

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“…Early in the work with the skin equivalent, Bell and colleagues at MIT were the first to show that cultivated allogeneic cells could be used to populate engineered tissues without rejection. 51 The living skin equivalent developed at Organogenesis Inc. and used in clinical trials through phase III consisted of cultivated allogeneic cells and was reported not to provoke immune responses leading to rejection. 52 It is awaiting approval from the Food and Drug Administration (FDA).…”

Section: Bellmentioning

confidence: 99%

Strategy for the Selection of Scaffolds for Tissue Engineering

Bell¹

1995

Tissue Engineering

Self Cite

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A strategy for developing scaffolding materials for prostheses capable of inducing tissue regeneration is presented. It is based on knowledge of the stimuli cells need to carry out their programs of division, morphogenesis, and differentiation, that is, of tissue building. The source of the stimulation is shown to be the extracellular matrix (ECM), in or on which cells in tissues live. A capsule view of the molecular diversity and the supramolecular architecture of the ECM is given with the idea that it can be imitated. The desired properties of scaffolding materials assembled from biological and engineering considerations allow the reader to evaluate biomaterials available for creating prostheses. Keeping in mind the ideal properties sought, a series of case histories is presented as a vehicle for examining prosthetic devices, and the materials used for them. Engineered or processed, naturally occurring, tissue replacements for articular cartilage, small caliber artery, periodontal tissues, cruciate ligament, and skin are reviewed from the point of view of how cells react to the scaffolds and how effectively a functional tissue replacement is restored in vivo. An approach to creating scaffolds by deducing their structure mathematically from their function in vivo is discussed as a first step in imitating the design of a body part. Finally touched on is the task of creating information-rich scaffolds like the ECM.

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Acceptance of Allogeneic Fibroblasts in Skin Equivalent Transplants

Cited by 73 publications

References 0 publications

Efficient retrovirus-mediated transfer and expression of a human adenosine deaminase gene in diploid skin fibroblasts from an adenosine deaminase-deficient human.

Efficient retrovirus-mediated transfer and expression of a human adenosine deaminase gene in diploid skin fibroblasts from an adenosine deaminase-deficient human.

Comparison of the Application of Allogeneic Fibroblast and Autologous Mesh Grafting With the Conventional Method in the Treatment of Third-Degree Burns

Strategy for the Selection of Scaffolds for Tissue Engineering

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