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ACCELERATION of wound healing produced by Mersilk. After an interval of 7 days, called 'the preliminary wounding was first observed by Botsford re-suture interval', the animals were re-anaesthetized, (I941), working in the Wilkie Surgical Research the sutures were removed and the wounds re-opened Laboratory in Edinburgh. The finding was confirmed by blunt dissection to undo any previous healing. by Young, Fisher, and Young (1941)~ Sandblom and These wounds were then re-sutured and termed Muren (1954)~ and Savlov and Dunphy (1954). 'healing secondary wounds'. At the same time as Douglas (1959) found that disrupted and fresh wounds these wounds were re-sutured, four fresh wounds I-cm. WOUND Dried under vacuum and over CaC1, DRIED WOUND I I Homogenized with 0.45 molar NaCl and allowed to stand for 6 days at 4 ' C., then centrifuged I SUPER~ATANT Added C,HSOH to 50 per cent concentration and allowed to stand at 4" C. for 48 hr., then centrifuged I RES~DUERefluxed with 5-5 per cent TCA at 9o°C. for 6 hr., then centrifuged I PRECIPITATE SUPERNATANT RESIDUE Collagen-hydrolysed Hydrolysed with 6N with 6N HC1 for 16 hr. HC1 for 16 hr. SUPERNATANT HC1 for 16 hr. Hydrolysed with 6N Sol. collagen-hydrolysed with 6N HC1 for 16 hr.Analyses: I, Nitrogen; 2, Proline; 3, Hydroxyproline. FIG. 1.-Diagram of processes involved in chemical fractionations of skin wounds in rabbits, preparatory to estimation of nitrogen, proline, and hydroxyproline.healing simultaneously in the same animal showed different rates of gain of tensile strength. The disrupted wounds healed more quickly than the fresh wounds.The present investigation was designed to study collagen synthesis in disrupted and fresh wounds. EXPERIMENTAL METHODSFour groups of rabbits were used. In Group I, four initial skin wounds were made on the left side of the back of each of the rabbits and sutured with were made and sutured on the right side of each of the rabbits' backs. These latter wounds were referred to as the ' corresponding primary wounds '. Healing for both these types of wounds is synchronous and considered to start from the day of re-suturing, since any previous healing had been undone on opening the original wounds. These final eight wounds were allowed to heal for 7 days, after which the rabbits were killed by an overdose of nembutal, and strips of the wounds excised using a double-bladed scalpel with blades set I cm. apart. After removing the
ACCELERATION of wound healing produced by Mersilk. After an interval of 7 days, called 'the preliminary wounding was first observed by Botsford re-suture interval', the animals were re-anaesthetized, (I941), working in the Wilkie Surgical Research the sutures were removed and the wounds re-opened Laboratory in Edinburgh. The finding was confirmed by blunt dissection to undo any previous healing. by Young, Fisher, and Young (1941)~ Sandblom and These wounds were then re-sutured and termed Muren (1954)~ and Savlov and Dunphy (1954). 'healing secondary wounds'. At the same time as Douglas (1959) found that disrupted and fresh wounds these wounds were re-sutured, four fresh wounds I-cm. WOUND Dried under vacuum and over CaC1, DRIED WOUND I I Homogenized with 0.45 molar NaCl and allowed to stand for 6 days at 4 ' C., then centrifuged I SUPER~ATANT Added C,HSOH to 50 per cent concentration and allowed to stand at 4" C. for 48 hr., then centrifuged I RES~DUERefluxed with 5-5 per cent TCA at 9o°C. for 6 hr., then centrifuged I PRECIPITATE SUPERNATANT RESIDUE Collagen-hydrolysed Hydrolysed with 6N with 6N HC1 for 16 hr. HC1 for 16 hr. SUPERNATANT HC1 for 16 hr. Hydrolysed with 6N Sol. collagen-hydrolysed with 6N HC1 for 16 hr.Analyses: I, Nitrogen; 2, Proline; 3, Hydroxyproline. FIG. 1.-Diagram of processes involved in chemical fractionations of skin wounds in rabbits, preparatory to estimation of nitrogen, proline, and hydroxyproline.healing simultaneously in the same animal showed different rates of gain of tensile strength. The disrupted wounds healed more quickly than the fresh wounds.The present investigation was designed to study collagen synthesis in disrupted and fresh wounds. EXPERIMENTAL METHODSFour groups of rabbits were used. In Group I, four initial skin wounds were made on the left side of the back of each of the rabbits and sutured with were made and sutured on the right side of each of the rabbits' backs. These latter wounds were referred to as the ' corresponding primary wounds '. Healing for both these types of wounds is synchronous and considered to start from the day of re-suturing, since any previous healing had been undone on opening the original wounds. These final eight wounds were allowed to heal for 7 days, after which the rabbits were killed by an overdose of nembutal, and strips of the wounds excised using a double-bladed scalpel with blades set I cm. apart. After removing the
The problem of the biochemical quantification of long term human wound healing was approached by measuring collagen synthesis in reincisions using specific radioimmunoassays for the wound fluid concentrations of the carboxyterminal propeptide of type I procollagen (PICP) and the aminoterminal propeptide of type III procollagen (PIIINP). First-day wound fluid PICP concentration after reincising a 3 week old scar was 25 times higher than the mean value in 20 reference standard incisions but scars older than 3 months did not show this difference. Wound fluid taken in subsequent days demonstrated that the initial acceleration of synthesis disappeared by the fourth day. When wound fluid PIIINP was assessed, high concentrations were found in reincisions of wounds for up to 5 months after the previous operation. The acceleration was also lost more slowly during the first postoperative week. The duration of a high rate of type I collagen synthesis compares well with studies in experimental wounds which show increased gain of strength if they are made not more than 6 weeks after previous surgery. The longer activity of the metabolism of type III collagen related antigens could reflect their function in the regulation of collagen fibril formation.
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