Acceleration of wound healing by ultrasound activation of TiO₂ in Escherichia coli‐infected wounds in mice

Abstract

Surgical site infections continue to be a common complication affecting surgical prognosis. Reactive oxygen species (ROS) are generated by ultrasound-irradiated titanium dioxide (TiO ) (UIT). Although excessive ROS production can cause cell damage, ROS at physiological levels mediate beneficial cellular responses, including angiogenesis. This study investigated whether UIT can promote healing of Escherichia coli-infected wounds. We used TiO and ultrasound irradiation using an ultrasonography machine at a frequency of 1.0 MHz and intensity of 0.4 W cm . These levels are not bactericidal in vitro; therefore, we could study the effect of UIT on E. coli survival without interference of ultrasound effects. The number of cluster of differentiation 31-positive blood vessels, which are indicative of angiogenesis, was decreased by bacterial infection, and increased at the wound edges in the UIT-treated infected wounds, suggesting upregulation of neovascularization by UIT. Although UIT treatment did not decrease E. coli survival in vivo, it promoted healing of the infected wounds as evidenced by a significant decrease in the wound area in the UIT-treated mice. Our findings demonstrate that UIT promotes wound healing in surgical site infections and suggest beneficial use of the UIT-based approach as a novel therapeutic method to treat infected surgical wounds. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2344-2351, 2017.

Titanium Nanorods Loaded PCL Meshes with Enhanced Blood Vessel Formation and Cell Migration for Wound Dressing Applications

“…This in vivo data is comparable with the results of the CAM assay (Figure ). In support to our data, earlier study showed that simultaneous application of ultrasound and TiO 2 nanoparticles promoted neovascularization in mice models . The most plausible reason for the enhanced wound healing could be due to the ability of TiO 2 nanoparticles to generate ROS in biological system .…”

The Synergistic Effect of Nanocrystals Combined With Ultrasound in the Generation of Reactive Oxygen Species for Biomedical Applications

“…It is also been proven that the ROS generation achieved by the external activation of membrane-bound NADPH oxidase can induce angiogenesis and other essential functions of endothelial cells, such as hemostasis (Kim et al, 2017;Aldosari et al, 2018). The activation of angiogenesis caused by an increase in ROS production to restore ROS physiological levels, can be beneficial not only for CVD, as occurs after ischemia (Urao et al, 2008), but can also contribute to wound healing (Osumi et al, 2017). On the other hand, an excessive production of ROS leads to a disequilibrium redox state, where the antioxidant defenses of the cell has been overcome, being responsible for damaging cellular components, as lipids, proteins and DNA.…”

Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing

“…Ultrasound therapy could enhance the penetration of NP into the tissues to treat deeper infected sites. Figure 10 shows (a) images of the infected wounds treated with or without using ultrasound-activated TiO 2 NPs, (b) curves illustrating relative wound size percentage calculated compared to the initial wound size [304].…”