Acceleration of Hepatic Cholesterol Synthesis by Triton Wr-1339

Frantz, Ivan D.; Hinkelman, Beverly T.

doi:10.1084/jem.101.3.225

Cited by 85 publications

(21 citation statements)

References 8 publications

(1 reference statement)

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“…Furthermore, the liver can produce greatly increased quantities of cholesterol following the injection of triton, as is shown in the studies of Frantz and Hinkelman who noted that liver slices from rats given triton incorporated radioactive acetate into cholesterol at a rate three times that of normal (8). Yet, extrahepatic synthesis of cholesterol may also occur (9), and it is manifestly possible that other tissues, which have the ability to synthesize cholesterol but under normal conditions contribute relatively little to the cholesterol pool, might, under the influence of triton, greatly accelerate their rates of synthesis.…”

Section: Discussionmentioning

confidence: 76%

The Pathogenesis of Hyperlipemia Induced by Means of Surface-Active Agents

Hirsch

Kellner

1956

The Journal of Experimental Medicine

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Mice rendered hyperlipemic by means of intravenous or subcutaneous injections of triton WR 1339 were found to have an increase in the total amount of cholesterol in their bodies. This observation indicates that the injected surface-active agent affects the metabolism of cholesterol and brings about hyperlipemia by augmenting the synthesis of lipides, or by interfering with their degradation or excretion, or by some combination of these factors. The implications of the findings for the pathogenesis of the hyperlipemia induced by triton are discussed further in the accompanying paper (16).

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Section: Discussionmentioning

confidence: 76%

The Pathogenesis of Hyperlipemia Induced by Means of Surface-Active Agents

Hirsch

Kellner

1956

The Journal of Experimental Medicine

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“…This finding suggests the possibility that cholesterol feedback control in the liver may be more responsive to exogenous than to endogenous cholesterol, a suggestion which is supported by the observation that in at least two other states characterized by hypercholesterolemia of endogenous origin, cholesterol synthesis is apparently not inhibited. Frantz and Hinkelman (11 ) have shown that during the elevation of serum cholesterol produced by injecting the deter-gent Triton WR-1339, cholesterol synthesis is increased; and Marsh and Drabkin (12) have reported that in the first 2 days following the induction of experimental nephrosis in the rat, the resulting moderate hypercholesterolemia does not cause a decrease in hepatic cholesterol synthesis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Endogenous and Exogenous Cholesterol on the Feedback Control of Cholesterol Synthesis*

Sakakida¹,

Shediac²,

Siperstein³

1963

J. Clin. Invest.

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“…(7). The explanation may be that, to purify the peroxisomes, our rats were pre-treated with Triton WR-1339, which is known to produce a hypercholesterolemic serum (22,23), as well as an accumulation of cholesterol in hepatic lysosomes (24) . Since the livers were not perfused, cholesterol might have adsorbed onto the membranes during homogenization .…”

Section: Comparison Of Membranesmentioning

confidence: 99%

Polypeptide and phospholipid composition of the membrane of rat liver peroxisomes: comparison with endoplasmic reticulum and mitochondrial membranes.

Fujiki¹,

Fowler²,

Shio³

et al. 1982

The Journal of Cell Biology

363

158

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Membranes were isolated from highly purified peroxisomes, mitochondria, and rough and smooth microsomes of rat liver by the one-step Na2CO3 procedure described in the accompanying paper (1982, J. Cell Biol . 93 :97-102) . The polypeptide compositions of these membranes were determined by SDS PAGE and found to be greatly dissimilar . The peroxisomal membrane contains 12% of the peroxisomal protein and consists of three major polypeptides (21,700, 67,700 and 69,700 daltons) as well as some minor polypeptides . The major peroxisomal membrane proteins as well as most of the minor ones are absent from the endoplasmic reticulum (ER) . Conversely, most ER proteins are absent from peroxisomes.By electron microscopy, purified peroxisomal membranes are -6 .8 nm thick and have a typical trilaminar appearance . The phospholipid/protein ratio of peroxisomal membranes is -200 nmol/mg; the principal phospholipids are phosphatidyl choline and phosphatidyl ethanolamine, as in ER and mitochondrial membranes. In contrast to the mitochondria, peroxisomal membranes contain no cardiolipin . All the membranes investigated contain a polypeptide band with a molecular mass of 15,000 daltons. Whether this represents an exceptional common membrane protein or a coincidence is unknown. The implications of these results for the biogenesis of peroxisomes are discussed.Knowledge of the peroxisomal membrane's properties is essential to an understanding both of the organelle's functions and of its biogenesis . The membrane separates the peroxisomal contents from the cytosol and defines the peroxisomal interior as a distinct intracellular space. The permeability properties of the membrane determine to what extent the peroxisome functions as a separate biochemical compartment. Knowledge of how the membrane is formed is essential to an understanding of the biogenesis of the organelle as a whole. If the membrane is derived from some other intracellular membrane, for example the endoplasmic reticulum (ER) (as is widely assumed), then one might expect to see some similarity in composition between them . If, on the other hand, the peroxisomes exist as a separate intracellular compartment, as has recently been suggested (1), then the peroxisomal membrane needs to have no structural similarity to the ER . THE JOURNAL OF CELL BIOLOGY " VOLUME 93 APRIL 1982 103-110 ©The Rockefeller University Press -0021-9525/82/04/0103/08 $1 .00We have applied the newly-developed sodium carbonate procedure described in the accompanying paper (2) to isolate peroxisomal, mitochondrial, and ER membranes.' We have partially characterized these three membranes, and found that their polypeptide compositions are almost entirely different, but their phospholipid compositions are similar. Some of these results have appeared in abstract form (3, 4) .' Rat liver microsomes were subfractionated by isopycnic centrifugation in linear sucrose gradients according to Beaufay et al. (7). The fractions selected as the "rough microsomal fraction" have been shown by these authors to c...

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Acceleration of Hepatic Cholesterol Synthesis by Triton Wr-1339

Cited by 85 publications

References 8 publications

The Pathogenesis of Hyperlipemia Induced by Means of Surface-Active Agents

The Pathogenesis of Hyperlipemia Induced by Means of Surface-Active Agents

Effect of Endogenous and Exogenous Cholesterol on the Feedback Control of Cholesterol Synthesis*

Polypeptide and phospholipid composition of the membrane of rat liver peroxisomes: comparison with endoplasmic reticulum and mitochondrial membranes.

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