Accelerating SARS-CoV-2 genomic surveillance in a routine clinical setting with nanopore sequencing

Buenestado-Serrano, Sergio; Herranz, Marta; Otero-Sobrino, Álvaro; Molero-Salinas, Andrea; Rodríguez-Grande, Cristina; Sanz-Pérez, Amadeo; Durán Galván, María José; Catalán, Pilar; Alonso, Roberto; Muñoz, Patricia; Pérez-Lago, Laura; García de Viedma, Darío

doi:10.1016/j.ijmm.2024.151599

Cited by 1 publication

(1 citation statement)

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“…Their instrumentation, the corresponding chemicals, and flow cells are more affordable, and the generated data are more targeted [ 131 ]. On the other hand, as long-read techniques offer a wider genomic picture, thus providing a deeper insight into nucleic acid traits, their introduction into routine examinations has started [ 132 – 136 ] and their spreading is expected in the near future.…”

Section: Applications Of Long-read Sequencingmentioning

confidence: 99%

Can long-read sequencing tackle the barriers, which the next-generation could not? A review

Szakállas,

Barták,

Valcz

et al. 2024

Pathol. Oncol. Res.

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The large-scale heterogeneity of genetic diseases necessitated the deeper examination of nucleotide sequence alterations enhancing the discovery of new targeted drug attack points. The appearance of new sequencing techniques was essential to get more interpretable genomic data. In contrast to the previous short-reads, longer lengths can provide a better insight into the potential health threatening genetic abnormalities. Long-reads offer more accurate variant identification and genome assembly methods, indicating advances in nucleotide deflect-related studies. In this review, we introduce the historical background of sequencing technologies and show their benefits and limits, as well. Furthermore, we highlight the differences between short- and long-read approaches, including their unique advances and difficulties in methodologies and evaluation. Additionally, we provide a detailed description of the corresponding bioinformatics and the current applications.

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Section: Applications Of Long-read Sequencingmentioning

confidence: 99%