Accelerating itaconic acid production by increasing membrane permeability of whole-cell biocatalyst based on a psychrophilic bacterium Shewanella livingstonensis Ac10

Luo, Gonglinfeng; Fujino, Mitsuhiro; Nakano, Sho; Hida, Akiko; Tajima, Takahisa; Kato, Junichi

doi:10.1016/j.jbiotec.2020.03.003

Cited by 18 publications

(12 citation statements)

References 24 publications

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“…Generally, in the two-step reaction of the IA synthesis process, the second step catalyzed by CAD was considered as the rate-limiting step, so more attention was focused on enhancing CAD expression by increasing the copy number of cadA or inserting cadA into the upstream of acn. 4,49 However, different from previous reports, our results suggested that the rate-limiting step might be the first step catalyzed by ACN. To test the catalytic efficiency of the first step from citrate to cis-aconitate, TD1.0-acn (TD1.0 with plasmid pN59-acn expression) was constructed and used to catalyze citrate to cis-aconitate.…”

Section: Construction Of the Recombinant Ia Biosynthesiscontrasting

confidence: 99%

“…According to previous reports, CAD was considered as the bottleneck of the IA synthesis process. ,, However, our results suggested that ACN was the rate-limiting step of IA synthesis in H. bluephagenesis (Figure ), and further increasing the copy number of acn improved ACN activity (Figure S5) and catalytic efficiency (Figure A).…”

Section: Discussionsupporting

confidence: 61%

“…coli, , C. glutamicum, and Shewanella livingstonensis . However, the intrinsic properties of CAD determine that it has low stability and tends to be expressed as insoluble aggregates in surrogate hosts, resulting in far lower IA titers than those obtained by fermentation of A.…”

Section: Discussionmentioning

confidence: 99%

“…The cadA from A. terreus has enabled IA production in a variety of heterologous hosts, including fungi such as A. niger, 52,53 S. cerevisiae, 27 Y. lipolytica, 26 and Candida lignohabitans, 54 and bacteria such as E. coli, 9,29 C. glutamicum, 28 and Shewanella livingstonensis. 49 However, the intrinsic properties of CAD determine that it has low stability and tends to be expressed as insoluble aggregates in surrogate hosts, resulting in far lower IA titers than those obtained by fermentation of A. terreus. 18 Not surprisingly, the poor expression and insolubility of CAD also existed in H. bluephagenesis (Figure S1).…”

Section: ■ Discussionmentioning

confidence: 99%

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Cell Catalysis of Citrate to Itaconate by Engineered Halomonas bluephagenesis

et al. 2021

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Itaconic acid (IA), an important five-carbon unsaturated dicarboxylic acid, is one of the top 12 renewable chemicals with an urgent need to reduce industrial production costs. Halomonas bluephagenesis, which possesses the potential for cost-effective bioproduction of chemicals and organic acids due to its ability to grow under open nonsterile conditions and high tolerance to organic acid salts, was genetically engineered and used to produce IA from citrate by a cell catalytic strategy. Here, two essential genes (cis-aconitate decarboxylase encoding gene cadA and aconitase (ACN) encoding gene acn) were introduced into H. bluephagenesis to construct an IA biosynthesis pathway. Further engineering modifications including coexpression of molecular chaperones GroESL, increasing the copy number of the gene encoding rate-limiting enzyme ACN, and weakening the competing pathway were implemented. Under the optimized condition for the cell catalytic system, the engineered strain TAZI-08 produced 451.45 mM (58.73 g/L) IA from 500 mM citrate, with 93.24% conversion in 36 h and a productivity of 1.63 g/(L h). An intermittent feeding strategy further increased the IA titer to 488.86 mM (63.60 g/L). The IA titer and citrate conversion in H. bluephagenesis are the highest among heterologous hosts reported so far, demonstrating that this strain is a suitable chassis for hyperproduction of IA.

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Section: Construction Of the Recombinant Ia Biosynthesiscontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

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Cell Catalysis of Citrate to Itaconate by Engineered Halomonas bluephagenesis

et al. 2021

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“…can produce this organic acid, A. terreus remains the most considerable strain, with the highest IA yield ( Table 1 ) [ 39 , 45 , 46 ]. Also, different microorganisms able to produce this building block chemical are species from Candida, Pseudozyma with a quantity of 30 g/L [ 47 ], Ustilaginaceae with values between 33–74.9 g/L [ 48 , 49 , 50 ], and others with insignificant quantity [ 51 , 52 ]. Besides biological fermentation, IA can also be produced through chemical techniques, fossil fuel.…”

Section: Itaconic Acid (Ia) Synthesismentioning

confidence: 99%

Recent Advances in Biotechnological Itaconic Acid Production, and Application for a Sustainable Approach

Teleky

Vodnar

2021

Polymers

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Intense research has been conducted to produce environmentally friendly biopolymers obtained from renewable feedstock to substitute fossil-based materials. This is an essential aspect for implementing the circular bioeconomy strategy, expressly declared by the European Commission in 2018 in terms of “repair, reuse, and recycling”. Competent carbon-neutral alternatives are renewable biomass waste for chemical element production, with proficient recyclability properties. Itaconic acid (IA) is a valuable platform chemical integrated into the first 12 building block compounds the achievement of which is feasible from renewable biomass or bio-wastes (agricultural, food by-products, or municipal organic waste) in conformity with the US Department of Energy. IA is primarily obtained through fermentation with Aspergillus terreus, but nowadays several microorganisms are genetically engineered to produce this organic acid in high quantities and on different substrates. Given its trifunctional structure, IA allows the synthesis of various novel biopolymers, such as drug carriers, intelligent food packaging, antimicrobial biopolymers, hydrogels in water treatment and analysis, and superabsorbent polymers binding agents. In addition, IA shows antimicrobial, anti-inflammatory, and antitumor activity. Moreover, this biopolymer retains qualities like environmental effectiveness, biocompatibility, and sustainability. This manuscript aims to address the production of IA from renewable sources to create a sustainable circular economy in the future. Moreover, being an essential monomer in polymer synthesis it possesses a continuous provocation in the biopolymer chemistry domain and technologies, as defined in the present review.

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