Accelerated wound healing using three-dimensional amniotic membrane scaffold in combination with adipose-derived stem cells in a diabetic rat model

Nasiry, Davood; Khalatbary, Ali Reza; Noori, Afshin; Abouhamzeh, Beheshteh; Jamalpoor, Zahra

doi:10.1016/j.tice.2023.102098

Cited by 6 publications

(3 citation statements)

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“…The pathway of p38 MAPK signaling plays an important role in controlling various cellular functions, mainly inflammation. 56 Several previous studies have presented the utility of D-hAM in wound healing, 33,57,58 the present data corroborates the claim by providing its proteomic basis and the potential wound healing pathways modulated by D-hAM.…”

Section: Discussionsupporting

confidence: 87%

“…hAM has been used in tissue engineering as a biological scaffold in the form of either fresh, cryopreserved or minimally processed tissue implants since the early 21st century. In recent years, it has been tested for the treatment of burns, 32 wounds, 33 and ophthalmological disorders 34 because of its low immunogenicity, regenerative capability, anti‐scarring, and anti‐inflammatory properties. The preparation of D‐hAM further enhances hAM's clinical utility as it minimizes the risk of implant rejection because of the host immune response.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of the proteome profile of decellularized human amniotic membrane and its biocompatibility with umbilical cord‐derived mesenchymal stem cells

Ahmed,

Tauseef,

Ainuddin

et al. 2024

J Biomedical Materials Res

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Extracellular matrix‐based bio‐scaffolds are useful for tissue engineering as they retain the unique structural, mechanical, and physiological microenvironment of the tissue thus facilitating cellular attachment and matrix activities. However, considering its potential, a comprehensive understanding of the protein profile remains elusive. Herein, we evaluate the impact of decellularization on the human amniotic membrane (hAM) based on its proteome profile, physicochemical features, as well as the attachment, viability, and proliferation of umbilical cord‐derived mesenchymal stem cells (hUC‐MSC). Proteome profiles of decellularized hAM (D‐hAM) were compared with hAM, and gene ontology (GO) enrichment analysis was performed. Proteomic data revealed that D‐hAM retained a total of 249 proteins, predominantly comprised of extracellular matrix proteins including collagens (collagen I, collagen IV, collagen VI, collagen VII, and collagen XII), proteoglycans (biglycan, decorin, lumican, mimecan, and versican), glycoproteins (dermatopontin, fibrinogen, fibrillin, laminin, and vitronectin), and growth factors including transforming growth factor beta (TGF‐β) and fibroblast growth factor (FGF) while eliminated most of the intracellular proteins. Scanning electron microscopy was used to analyze the epithelial and basal surfaces of D‐hAM. The D‐hAM displayed variability in fibril morphology and porosity as compared with hAM, showing loosely packed collagen fibers and prominent large pore areas on the basal side of D‐hAM. Both sides of D‐hAM supported the growth and proliferation of hUC‐MSC. Comparative investigations, however, demonstrated that the basal side of D‐hAM displayed higher hUC‐MSC proliferation than the epithelial side. These findings highlight the importance of understanding the micro‐environmental differences between the two sides of D‐hAM while optimizing cell‐based therapeutic applications.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Assessment of the proteome profile of decellularized human amniotic membrane and its biocompatibility with umbilical cord‐derived mesenchymal stem cells

Ahmed,

Tauseef,

Ainuddin

et al. 2024

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Despite the advantageous clinical results obtained by grafting AM, there are some challenges for surgeons to handle thin AM tissue without tearing or folding, which limits the incorporation of fresh AM into routine clinical applications to some extent ( Murphy et al, 2017 ; Dadkhah Tehrani et al, 2021 ). Besides, the dense structure of the AM tissue is known to limit the penetration of cells to the wounded site ( Nasiry et al, 2021 ; Nasiry et al, 2022 ; Khalatbary et al, 2023 ; Nasiry et al, 2023 ). One of the methods to solve these issues was to use its dehydrated form, which is easier to handle and can be stored at room temperature with a shelf life of about 5 years ( Fetterolf and Snyder, 2012 ).…”

Section: Introductionmentioning

confidence: 99%