Accelerated wound healing in diabetic mice by miRNA-497 and its anti-inflammatory activity

Ban, Eunmi; Jeong, Seonghee; Park, Mijung; Kwon, Hyun Joo; Park, Jin‐Young; Song, Eun Joo; Kim, Aeri

doi:10.1016/j.biopha.2019.109613

Cited by 79 publications

(53 citation statements)

References 32 publications

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“…miRNAs are important regulators in the body. A large number of studies have shown that they play an important role in many diseases by targeting target genes to regulate cell apoptosis, proliferation, oxidative stress, and the release of inflammatory factors [ 32 – 35 ]. In recent years, an increasing number of studies have shown that miR-25-3p plays an important regulatory role in IRI.…”

Section: Discussionmentioning

confidence: 99%

miR-25-3p protects renal tubular epithelial cells from apoptosis induced by renal IRI by targeting DKK3

Zhang

Zuo

2021

Open Life Sciences

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Renal ischemia-reperfusion injury (IRI) is one of the main causes of acute kidney injury (AKI). So far, there have been many studies on renal IRI, although an effective treatment method has not been developed. In recent years, growing evidence has shown that small noncoding RNAs play an important regulatory role in renal IRI. This article aims to explore whether microRNA-25-3p (miR-25-3p) plays a role in the molecular mechanism of renal IRI. The results showed that the expression level of miR-25-3p was significantly downregulated in a rat renal IRI model, and this result was confirmed with in vitro experiments. After the hypoxia-reoxygenation treatment, the apoptosis level of NRK-52E cells transfected with miR-25-3p mimics decreased significantly, and this antiapoptotic effect was antagonized by miR-25-3p inhibitors. In addition, we confirmed that DKK3 is a target of miR-25-3p. miR-25-3p exerts its protective effect against apoptosis on NRK-52E cells by inhibiting the expression of DKK3, and downregulating the expression level of miR-25-3p could disrupt this protective effect. In addition, we reconfirmed the role of miR-25-3p in rats. Therefore, we confirmed that miR-25-3p may target DKK3 to reduce renal cell damage caused by hypoxia and that miR-25-3p may be a new potential treatment for renal IRI.

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Section: Discussionmentioning

confidence: 99%

miR-25-3p protects renal tubular epithelial cells from apoptosis induced by renal IRI by targeting DKK3

Zhang

Zuo

2021

Open Life Sciences

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“…98 In vivo and in vitro studies have shown that miR-497 can reduce the levels interleukin (IL)-1b, IL-6, TNF-a, and other pro-inflammatory factors and promote wound epithelialization and granulation tissue formation, thereby accelerating the diabetic wounds-healing process. 70 Furthermore, miR-146a was significantly downregulated during the healing process of diabetic wounds, leading to a significant increase in the downstream pro-inflammatory targets IRAK1, TRAF6, and NF-kB and an increase in IL-6 and MIP-2 gene expression. In addition, mesenchymal stem cells (MSCs) accelerate wound healing and relieve inflammation by increasing the expression of miR-146a in diabetic wounds.…”

Section: Anti-inflammatory Effectsmentioning

confidence: 99%

The progress, prospects, and challenges of the use of non-coding RNA for diabetic wounds

Wei

Liu

et al. 2021

Molecular Therapy - Nucleic Acids

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Chronic diabetic wounds affect the quality of life of patients, resulting in significant social and economic burdens on both individuals and the health care system. Although treatment methods for chronic diabetic wounds have been explored, there remains a lack of effective treatment strategies; therefore, alternative strategies must be explored. Recently, the abnormal expression of non-coding RNA in diabetic wounds has received widespread attention since it is an important factor in the development of diabetic wounds. This article reviews the regulatory role of three common non-coding RNAs (microRNA [miRNA], long non-coding RNA [lncRNA], and circular RNA [circRNA]) in diabetic wounds and discusses the diagnosis, treatment potential, and challenges of non-coding RNA in diabetic wounds. This article provides insights into new strategies for diabetic wound diagnosis and treatment at the genetic and molecular levels. ncRNA ncRNA refers to a type of RNA that does not encode protein. In the past, it has been shown to participate in a variety of biological processes and play important transcriptional and post-transcriptional regulatory functions. 18 ncRNAs are divided into two categories based on the length of the RNA chain; lncRNA have lengths greater than 200 nucleotides, and small ncRNAs (snRNA) have lengths less than 200 nucleotides. snRNAs can be subdivided into miRNAs and circRNAs. 19 miRNAs inhibit the expression of target genes by binding to the 3 0 untranslated region (3 0 UTR) of the target mRNA. 20 The miRNA gene is processed and cleaved by RNA polymerase II (Pol II) enzyme and endonuclease Drosha III to produce pre-miRNA, which is

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“…This suggests that targeting miR-320 may represent a potential therapeutic strategy to treat diabetes-induced cardiac dysfunction. A recent study has identified miRNA-497 as a potential therapeutic agent for diabetic wound healing, owing to its repressive effect on proinflammatory cytokines (Ban et al, 2020). Furthermore, some studies indicate that as circulating miRNAs can be altered depending on the phase of the disease, they could also be used as potential biomarkers for assessing the development and progression of DCM.…”

Section: Clinical Application Of Small Rnas In Diabetic Cardiomyopathymentioning

confidence: 99%

Research Progress on Epigenetics of Diabetic Cardiomyopathy in Type 2 Diabetes

Deng

Liao

Jian-pin

et al. 2021

Front. Cell Dev. Biol.

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Diabetic cardiomyopathy (DCM) is characterized by diastolic relaxation abnormalities in its initial stages and by clinical heart failure (HF) without dyslipidemia, hypertension, and coronary artery disease in its last stages. DCM contributes to the high mortality and morbidity rates observed in diabetic populations. Diabetes is a polygenic, heritable, and complex condition that is exacerbated by environmental factors. Recent studies have demonstrated that epigenetics directly or indirectly contribute to pathogenesis. While epigenetic mechanisms such as DNA methylation, histone modifications, and non-coding RNAs, have been recognized as key players in the pathogenesis of DCM, some of their impacts remain not well understood. Furthering our understanding of the roles played by epigenetics in DCM will provide novel avenues for DCM therapeutics and prevention strategies.

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Accelerated wound healing in diabetic mice by miRNA-497 and its anti-inflammatory activity

Cited by 79 publications

References 32 publications

miR-25-3p protects renal tubular epithelial cells from apoptosis induced by renal IRI by targeting DKK3

miR-25-3p protects renal tubular epithelial cells from apoptosis induced by renal IRI by targeting DKK3

The progress, prospects, and challenges of the use of non-coding RNA for diabetic wounds

Research Progress on Epigenetics of Diabetic Cardiomyopathy in Type 2 Diabetes

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