Accelerated methanogenesis from effluents of hydrogen-producing stage in anaerobic digestion by mixed cultures enriched with acetate and nano-sized magnetite particles

Yang, Zhiman; Xu, Xiaohui; Guo, Rongbo; Fan, Xiaolei; Zhao, Xiaoxian

doi:10.1016/j.biortech.2015.04.057

Cited by 54 publications

(12 citation statements)

References 35 publications

(43 reference statements)

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“…Second, the cumulative relative abundance of acetogenic bacteria at the thermophilic temperature reached 7.09% higher than that observed in mesophilic conditions (4.83%). Thirdly, differentially abundant families at the TAnD reactor gathered in the methanogenic pathway, which constituted a hydrogenotrophic methanogen of Methanobacteriaceae , a hydrogenotrophic/methylotrophic of Methanomicrobiaceae , and three low-abundance microorganisms, including Enterobacteriaceae affiliated with hydrogen-producing processes [58], Planococcaceae associated with a high concentration of carbon source [59] and Pseudomonadaceae which could reduce Fe(III) with hydrogen or acetate [60]. In contrast, under mesophilic conditions, the differentially abundant bacteria were broadly located in hydrolysis, acidogenesis, and acetogenesis and only methanogens had significantly higher relative abundance in methanogenesis.…”

Section: Discussionmentioning

confidence: 99%

Inferring microbial interactions in thermophilic and mesophilic anaerobic digestion of hog waste

et al. 2017

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Anaerobic digestion (AnD) is a microbiological process that converts organic waste materials into biogas. Because of its high methane content, biogas is a combustible energy source and serves as an important environmental technology commonly used in the management of animal waste generated on large animal farms. Much work has been done on hardware design and process engineering for the generation of biogas. However, little is known about the complexity of the microbiology in this process. In particular, how microbes interact in the digester and eventually breakdown and convert organic matter into biogas is still regarded as a “black box.” We used 16S rRNA sequencing as a tool to study the microbial community in laboratory hog waste digesters under tightly controlled conditions, and systematically unraveled the distinct interaction networks of two microbial communities from mesophilic (MAnD) and thermophilic anaerobic digestion (TAnD). Under thermophilic conditions, the well-known association between hydrogen-producing bacteria, e.g., Ruminococcaceae and Prevotellaceae, and hydrotrophic methanogens, Methanomicrobiaceae, was reverse engineered by their interactive topological niches. The inferred interaction network provides a sketch enabling the determination of microbial interactive relationships that conventional strategy of finding differential taxa was hard to achieve. This research is still in its infancy, but it can help to depict the dynamics of microbial ecosystems and to lay the groundwork for understanding how microorganisms cohabit in the anaerobic digester.

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Section: Discussionmentioning

confidence: 99%

Inferring microbial interactions in thermophilic and mesophilic anaerobic digestion of hog waste

et al. 2017

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“…CH 4 and H 2 was periodically analyzed using a gas chromatograph 26 . Ethanol, individual VFAs, TS and VS were determined as described previously 24 . Concentrations of Fe (II) extracted with 0.5 N HCl were determined using a ferrozine method 27 .…”

Section: Methodsmentioning

confidence: 99%

“…The bottles were subsequently purged with high purity N 2 for 2 min and sealed with butyl rubber stoppers. The medium (pH 7.0) was composed of 20 mM ethanol, and other components were described previously 24 .…”

Section: Methane Production By Batch Culturementioning

confidence: 99%

“…Amplicon libraries were constructed for Illumina sequencing using primer pairs 515f/806r for bacteria 28 and 519f/ 825r for archaea 29 . PCR, library construction, Illumina sequencing and sequence analysis were performed as described previously 24 . The sequences were deposited to the GeneBank Sequence Read Archive with accession No.…”

Section: Dna Extraction Pcr Amplification and 16s Rdna Sequencingmentioning

confidence: 99%

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Magnetite nanoparticles facilitate methane production from ethanol via acting as electron acceptors

Yang

Shi

Wang

et al. 2015

Sci Rep

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Potential for interspecies hydrogen transfer within paddy soil enrichments obtained via addition of magnetite nanoparticles and ethanol (named as PEM) was investigated. To do this, PEM derived from rice field of Hangzhou (named as PEM-HZ) was employed, because it offered the best methane production performance. Methane production and Fe (III) reduction proceeded in parallel in the presence of magnetite. Inhibition experiments with 2-bromoethane sulfonate (BES) or phosphate showed that interspecies hydrogen transfer and Fe (III) reduction also occurred in methane production from ethanol. 16S rRNA-based Illumina sequencing results showed that Dechloromonas, Thauera, Desulfovibrio and Clostridium were the dominant putative Fe (III) -reducers, and that hydrogenotrophic Methanobacterium accounted for about 88% of the total archaeal community. These results indicated that magnetite nanoparticles that acted as electron acceptor could facilitate rapid oxidation of ethanol by members of the Fe (III) -reducers in PEM-HZ and establishment of the syntrophic relationship of Fe (III) -reducers with Methanobacterium via interspecies hydrogen transfer. Our results could offer a model to understand the microbial interaction with magnetite from a novel angle during methanogenesis.

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“…Anaerobic digestion has been considered economical during treatment of complex organic wastes [ 18 ], and researchers are focused on developing the acidogenesis stage of H 2 production using mixed-culture that could be affordable and accessible [ 20 , 21 ]. Anaerobic digestion in the presence of mixed-culture have broader choice of organic waste feedstocks, and are easy to operate and control the growth of cultures during H 2 production [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Enrichment of Secondary Wastewater Sludge for Production of Hydrogen from Crude Glycerol and Comparative Evaluation of Mono-, Co- and Mixed-Culture Systems

Pachapur

Kutty

Brar

et al. 2016

IJMS

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Anaerobic digestion using mixed-culture with broader choice of pretreatments for hydrogen (H2) production was investigated. Pretreatment of wastewater sludge by five methods, such as heat, acid, base, microwave and chloroform was conducted using crude glycerol (CG) as substrate. Results for heat treatment (100 °C for 15 min) showed the highest H2 production across the pretreatment methods with 15.18 ± 0.26 mmol/L of medium at 30 °C in absence of complex media and nutrient solution. The heat-pretreated inoculum eliminated H2 consuming bacteria and produced twice as much as H2 as compared to other pretreatment methods. The fermentation conditions, such as CG concentration (1.23 to 24 g/L), percentage of inoculum size (InS) (1.23% to 24% v/v) along with initial pH (2.98 to 8.02) was tested using central composite design (CCD) with H2 production as response parameter. The maximum H2 production of 29.43 ± 0.71 mmol/L obtained at optimum conditions of 20 g/L CG, 20% InS and pH 7. Symbiotic correlation of pH over CG and InS had a significant (p-value: 0.0011) contribution to H2 production. The mixed-culture possessed better natural acclimatization activity for degrading CG, at substrate inhibition concentration and provided efficient inoculum conditions in comparison to mono- and co-culture systems. The heat pretreatment step used across mixed-culture system is simple, cheap and industrially applicable in comparison to mono-/co-culture systems for H2 production.

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Accelerated methanogenesis from effluents of hydrogen-producing stage in anaerobic digestion by mixed cultures enriched with acetate and nano-sized magnetite particles

Cited by 54 publications

References 35 publications

Inferring microbial interactions in thermophilic and mesophilic anaerobic digestion of hog waste

Inferring microbial interactions in thermophilic and mesophilic anaerobic digestion of hog waste

Magnetite nanoparticles facilitate methane production from ethanol via acting as electron acceptors

Enrichment of Secondary Wastewater Sludge for Production of Hydrogen from Crude Glycerol and Comparative Evaluation of Mono-, Co- and Mixed-Culture Systems

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