Accelerated design of bioconversion processes using automated microscale processing techniques

Lye, Gary J.; Ayazi-Shamlou, Parviz; Baganz, Frank; Dalby, Paul A.; Woodley, John M.

doi:10.1016/s0167-7799(02)00011-2

Cited by 128 publications

(99 citation statements)

References 56 publications

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“…An oxygen transfer rate (OTR) up to $350 mmol O 2 l )1 h )1 may be achieved with this reactor for aerobic cultures, which is a significant improvement in relation to OTRs of other micro-reactor systems such as e.g. deep square wells, with typical OTR values of 16-33 mmol O 2 l )1 h )1 (Lye et al 2003). In the present case this is beneficial to the metabolism of Y. lipolytica which is a strictly aerobic yeast species.…”

Section: The Experimental Biotransformation Set-upmentioning

confidence: 96%

“…Platform technologies can effectively decrease the time required to develop highproducing cell lines for biotherapeutics, as well discussed in two recent reviews (Lye et al 2003, Kumar et al 2004. Micro-reactors offer a cheap and fast tool to achieve an optimisation by a screening approach, of course not only in the pharmaceutical industry, but also possibly in other areas, such as the degradation of hydrophobic pollutants and food technology.…”

Section: Introductionmentioning

confidence: 99%

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Application of a Novel Oscillatory Flow Micro-bioreactor to the Production of γ-decalactone in a Two Immiscible Liquid Phase Medium

et al. 2006

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A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of c-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred tank bioreactor or shake flask). A three-fold increase in c-decalactone productivity was obtained by increasing oscillatory mixing intensity from Re o $482 to Re o $1447. This was presumably related to the effective contribution of the reactor geometry to enhanced mass transfer rates between the two immiscible liquid phases involved in the process by increasing the interfacial area.

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Section: The Experimental Biotransformation Set-upmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Application of a Novel Oscillatory Flow Micro-bioreactor to the Production of γ-decalactone in a Two Immiscible Liquid Phase Medium

et al. 2006

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“…A recent study developed an automated scaled-down method for optimizing the flocculation of pre-clarified cell broths [12]. These innovations are being driven primarily by the need within the biotechnology industry to minimize the occurrence of process bottlenecks and to bring the pace of development of downstream separation processes in line with the upstream product discoveries [13].…”

Section: Introductionmentioning

confidence: 99%

The microscale flocculation test (MFT)—A high-throughput technique for optimizing separation performance

LaRue

Cobbledick

Aubry

et al. 2016

Chemical Engineering Research and Design

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In this work, a new microscale flocculation test (MFT) method was developed that is ideally-suited for optimizing separation performance. A critical and complicated task in wastewater treatment is to identify the flocculation conditions that yield the optimal separation of water from suspended solid materials. The standard 'jar test' method is inadequate for conducting a full process optimization because a typical set-up only allows for a maximum of 6 tests to be conducted at once, and fairly large volumes of materials (approximately 1 to 2 litres) are needed for each individual test. The microplate-based, parallel processing format of the MFT method allows for many dozens of flocculation tests to be conducted simultaneously, with each test requiring only a few millilitres of material. As a demonstration of the MFT method, ten cationic polymer flocculants were evaluated with various digestate types. The optimal separation performance, as determined by the lowest capillary suction time (CST) measurement, was found by rigorously evaluating the effect of flocculant type (including molecular weight and charge density) and dosage conditions (including total amount added and single versus staged addition).For example, the dose-dependent profiles for certain flocculants exhibited a nearly 10-fold greater decrease in CST compared to other flocculants. Process optimization in environmental separations is not trivial, but rather, is a complicated task that requires an extensive amount of experimental work for which the MFT method is ideally suited. For any dewatering process, an ongoing challenge involves selecting, from the vast number of possible flocculants, the 'best' one in terms of economics and performance. In our previous work, we found that the dewatering performance of twenty-four different flocculants from three suppliers varied considerably [4]. Notably, for anaerobic wastewater treatment, the cost of the polymer flocculant is the greatest component in the biosolids treatment operation [5].This leads to two important considerations: first, it is generally understood that there is no workable algorithm to relate flocculant properties to dewatering performance [3]. Secondly, wastewater sources from different treatment plants have diverse dewatering characteristics. So, the exact flocculant type and the optimum dose must be determined on a case-by-case basis for different treatment applications. Yet, the selection of the 'best' flocculant has been described as "one of the most demanding, frustrating, and time-consuming experiences for many operations personnel" [6].The benefit of optimizing the flocculant dose extends beyond the operating costs for a given treatment facility. Water-soluble polymers have been found to be toxic to aquatic lifeforms, even at low concentrations [7]. Thus, there are valid concerns about the effect of flocculant 'overdosing' in dewatering processes, given the potential for discharge into the aquatic environment, either directly via the liquid fraction, or indirectly via leachin...

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“…To fully utilize the intrinsic advantages of biocatalysts, the development of techniques that promote better integration of biocatalysis into industrial production of bulk commodities and fine chemicals is of growing importance. [1][2][3] Microreactors are particularly attractive for multiphase reactions. The small diameter in the order of 100 m -1 mm yields efficient mass and heat transfer due to the large surface-to-volume ratio and allows for precise control of the reaction variables thus facilitating parallel rapid screening of reactions with minimal amounts of reagents.…”

Section: Introductionmentioning

confidence: 99%

“…5 In recent years, microchannel reactors have been extensively used in analysis and synthesis 6 as well as for the accelerated collection of data for the optimization of biocatalytic processes. 1,2 In this way, the speed of the bioprocess design can be improved and a wide range of variables can be examined in a short time.…”

Section: Introductionmentioning

confidence: 99%

cBSA-147 for the preparation of bacterial biofilms in a microchannel reactor

Jaenicke

Eisele

et al. 2010

Biointerphases

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Whole cells are attractive biocatalysts, particularly if the reaction requires cofactors or involves multiple transformations. Immobilization of the catalyst is often a prerequisite for continuous processes. The highly cationic chemically modified plasma protein bovine serum albumin ͑cBSA-147͒ has been applied for the electrostatically mediated immobilization of the planktonic bacterium E. coli BL21 star (DE3), and the resulting biofilms were superior to those formed on poly-L-lysine coated surfaces. The biocatalyst was immobilized in a capillary column ͑inside diameter of 530 m and L =30 m͒ and evaluated in the enantioselective reduction of ethyl acetoacetate to R-͑-͒ethyl hydroxybutyrate. In continuous operation in the microreactor format, the productivity of the cells was about 30% higher than that determined in a bench-scale fermentation system. This increase is attributed to the improved mass transfer over short geometrical dimensions. The similarity in the results indicates that studies on a biofilm-coated microreactor can be used for the accelerated collection of data for process optimization.

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Accelerated design of bioconversion processes using automated microscale processing techniques

Cited by 128 publications

References 56 publications

Application of a Novel Oscillatory Flow Micro-bioreactor to the Production of γ-decalactone in a Two Immiscible Liquid Phase Medium

Application of a Novel Oscillatory Flow Micro-bioreactor to the Production of γ-decalactone in a Two Immiscible Liquid Phase Medium

The microscale flocculation test (MFT)—A high-throughput technique for optimizing separation performance

cBSA-147 for the preparation of bacterial biofilms in a microchannel reactor

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