“…1 , scaffolds with a 0°/90° lay-down pattern architecture, 330 μm fibre diameter, and 350 μm top view and 210 μm side view pore size, were fabricated using 3D Discovery (regenHU, Switzerland), a screw-assisted material-extrusion additive manufacturing system, at room temperature and with the optimal processing parameters previously reported (nozzle diameter of 330 μm, printing temperature of 90 °C, air pressure of 6 bars, deposition velocity of 13 mm/s, and screw rotational velocity of 8 rpm, and layer thickness of 270 μm) [ [40] , [41] , [42] ]. The geometrical characteristics of the considered scaffolds were based on previous studies from our group that investigated the effect of filament diameter, pore size, and porosity on mechanical and biological properties [ [42] , [43] , [44] , [45] ]. As reported the considered values are the ones that allow both high mechanical properties and cell attachment, proliferation, and differentiation.…”