Accelerated bone ingrowth by local delivery of Zinc from bioactive glass: oxidative stress status, mechanical property, and microarchitectural characterization in an ovariectomized rat model

Jbahi, Samira; Saoudi, Mongi; Abdelmajid, Kabir; Oudadesse, Hassane; Treq, Rebai; Hafed, Efeki; Elfeki, Abdelfattah; Keskes, Hassib

doi:10.3402/ljm.v10.28572

Cited by 10 publications

(4 citation statements)

References 44 publications

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“…However, oral administration of zinc sulfate induced calcium contents in newborn rats [45]. In addition, when synthetic bone grafts incorporating zinc were implanted in the femoral condyles of ovariectomized rats, bone mass and bone mechanical properties were found to significantly improve in these animals [46]. Despite these previous studies, the mechanisms accounting for zinc-induced osteogenesis in hBMSCs had previously remained largely unclear.…”

Section: Figmentioning

confidence: 99%

Zinc Promotes Osteoblast Differentiation in Human Mesenchymal Stem Cells Via Activation of the cAMP-PKA-CREB Signaling Pathway

Park

Choi

Yoon

et al. 2018

Stem Cells and Development

104

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The crucial trace element zinc stimulates osteogenesis in vitro and in vivo. However, the pathways mediating these effects remain poorly understood. This study aimed to investigate the effects of zinc on osteoblast differentiation in human bone marrow-derived mesenchymal stem cells (hBMSCs) and to identify the molecular mechanisms of these effects. In hBMSCs, zinc exposure resulted in a dose-dependent increase in osteogenesis and increased mRNA and protein levels of the master transcriptional factor RUNX2. Analyzing the upstream signaling pathways of RUNX2, we found that protein kinase A (PKA) signaling inhibition blocked zinc-induced osteogenic effects. Zinc exposure increased transcriptional activity and protein levels of phospho-CREB and enhanced translocation of phospho-CREB into the nucleus. These effects were reversed by H-89, a potent inhibitor of PKA. Moreover, zinc exposure led to dose-dependent increases in levels of intracellular cyclic adenosine monophosphate (cAMP). These findings indicate that zinc activates the PKA signaling pathway by triggering an increase in intracellular cAMP, leading to enhanced osteogenic differentiation in hBMSCs. Our results suggest that zinc exerts osteogenic effects in hBMSCs by activation of RUNX2 via the cAMP-PKA-CREB signaling pathway. Zinc supplementation may offer a promise as a potential pharmaceutical therapy for osteoporosis and other bone loss conditions.

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Section: Figmentioning

confidence: 99%

Zinc Promotes Osteoblast Differentiation in Human Mesenchymal Stem Cells Via Activation of the cAMP-PKA-CREB Signaling Pathway

Park

Choi

Yoon

et al. 2018

Stem Cells and Development

104

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“…Oxidative stress has been involved in the pathogenesis of bone diseases, such as osteoporosis [130]. However, growing evidence shows that oxidative stress also mediates the process of bone remodelling and that excessive production and accumulation of ROS and LPO products in the bone tissue has a detrimental impact on bone metabolism [129,131].…”

Section: Glass and Ceramicmentioning

confidence: 99%

Biocompatibility of implantable materials: An oxidative stress viewpoint

et al. 2016

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Oxidative stress occurs when the production of oxidants surpasses the antioxidant capacity in living cells. Oxidative stress is implicated in a number of pathological conditions such as cardiovascular and neurodegenerative diseases but it also has crucial roles in the regulation of cellular activities. Over the last few decades, many studies have identified significant connections between oxidative stress, inflammation and healing. In particular, increasing evidence indicates that the production of oxidants and the cellular response to oxidative stress are intricately connected to the fate of implanted biomaterials. This review article provides an overview of the major mechanisms underlying the link between oxidative stress and the biocompatibility of biomaterials. ROS, RNS and lipid peroxidation products act as chemo-attractants, signalling molecules and agents of degradation during the inflammation and healing phases. As chemo-attractants and signalling molecules, they contribute to the recruitment and activation of inflammatory and healing cells, which in turn produce more oxidants. As agents of degradation, they contribute to the maturation of the extracellular matrix at the healing site and to the degradation of the implanted material. Oxidative stress is itself influenced by the material properties, such as by their composition, their surface properties and their degradation products. Because both cells and materials produce and react with oxidants, oxidative stress may be the most direct route mediating the communication between cells and materials. Improved understanding of the oxidative stress mechanisms following biomaterial implantation may therefore help the development of new biomaterials with enhanced biocompatibility.

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“…Strontium-doped BGs appear to guarantee better regenerative outcomes [ 79 ], as well as association with PRP or growth factors [ 59 , 76 ]. In particular, zinc seems to allow the control of the dissolution and replacement times of BGs in biological tissues; additionally, the release of zinc ions accelerates bone formation [ 104 , 105 ]. Another consideration concerns the use of membranes for GTR.…”

Section: Discussionmentioning

confidence: 99%

Bioactive Glasses in Periodontal Regeneration: Existing Strategies and Future Prospects—A Literature Review

et al. 2022

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The present review deals with bioactive glasses (BGs), a class of biomaterials renowned for their osteoinductive and osteoconductive capabilities, and thus widely used in tissue engineering, i.e., for the repair and replacement of damaged or missing bone. In particular, the paper deals with applications in periodontal regeneration, with a special focus on in vitro, in vivo and clinical studies. The study reviewed eligible publications, identified on the basis of inclusion/exclusion criteria, over a ranged time of fifteen years (from 1 January 2006 to 31 March 2021). While there are many papers dealing with in vitro tests, only a few have reported in vivo (in animal) research, or even clinical trials. Regardless, BGs seem to be an adequate choice as grafts in periodontal regeneration.

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Accelerated bone ingrowth by local delivery of Zinc from bioactive glass: oxidative stress status, mechanical property, and microarchitectural characterization in an ovariectomized rat model

Cited by 10 publications

References 44 publications

Zinc Promotes Osteoblast Differentiation in Human Mesenchymal Stem Cells Via Activation of the cAMP-PKA-CREB Signaling Pathway

Zinc Promotes Osteoblast Differentiation in Human Mesenchymal Stem Cells Via Activation of the cAMP-PKA-CREB Signaling Pathway

Biocompatibility of implantable materials: An oxidative stress viewpoint

Bioactive Glasses in Periodontal Regeneration: Existing Strategies and Future Prospects—A Literature Review

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