Accelerated blood clearance of PEGylated liposomes following preceding liposome injection: Effects of lipid dose and PEG surface-density and chain length of the first-dose liposomes

Ishida, Tatsuhiro; Harada, Masae; Wang, Xin Yu; Ichihara, Masako; Irimura, Kenji; Kojima, Hiroshi

doi:10.1016/j.jconrel.2005.04.003

Cited by 305 publications

(247 citation statements)

References 34 publications

Supporting

Mentioning

236

Contrasting

Unclassified

Order By: Relevance

“…However, the two main factors, namely, phospholipid (PL) types and animal models, have not yet been studied. Ishida et al (2005) found that when the first PL dose is larger than 1 mmolÁkg À1 , the second injection of PEGylated liposomes cannot induce the ABC phenomenon. By contrast, Laverman et al (2001) reported that an intravenous (i.v.)…”

Section: Introductionmentioning

confidence: 96%

“…The former study used hydrogenated egg phosphatidylcholine (HEPC), whereas the latter study used partially hydrogenated egg phosphatidylcholine. In this study on ABC phenomenon, HEPC, cholesterol and PEG-DSPE were usually used to prepare PEGylated liposomes (Laverman et al, 2001;Ishida et al, 2005Ishida et al, , 2006b, or hydrogenated soybean phosphatidylcholine (HSPC) was adopted instead of HEPC (Ishida et al, 2006a). As of this writing, no experimental studies have demonstrated the effects of PL types on the ABC phenomenon, and these effects are worthy of further investigation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of phospholipid types and animal models on the accelerated blood clearance phenomenon of PEGylated liposomes upon repeated injection

et al. 2014

Drug Delivery

View full text Add to dashboard Cite

(2015) Influence of phospholipid types and animal models on the accelerated blood clearance phenomenon of PEGylated liposomes upon repeated injection, Drug Delivery, 22:5, 598-607, DOI: 10.3109/10717544.2014 AbstractWhen polyethylene glycol (PEG)ylated liposomes were repeatedly injected into the same animal, the second dose of liposomes would rapidly clear from the bloodstream and enhance accumulation in the liver and spleen, and this phenomenon is called ''accelerated blood clearance (ABC)''. There are many factors known to influence ABC phenomenon, in this study, we mainly focused on the effects of different phospholipids (PL) types and animal models. The effects of PL types on ABC phenomenon were examined by repeating injection of PEGylated liposomes prepared by five different types of PL (hydrogenated soy phosphatidylcholine, egg sphingomyelin, soybean phosphatidycholin, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and egg phosphatidycholin) in rats. Dramatically, repeated injection of different types of PL could induce ABC phenomenon altogether. Both t 1/2 and AUC of experimental group (EG) were lower significantly than those of control group (CG). Our results also showed that the liver accumulation of second dose increased significantly (p50.01) in all EG as compared that of CG. Interestingly, ABC phenomenon of liposomes prepared by unsaturated PL was more obvious than that of saturated PL. All the first dose could induce the antibody (anti-PEG IgM) level increasing significantly (p50.01). For different animal models, we found that after repeated injection of PEGylated liposomes, rats, mice, rabbits and guinea pigs could produce ABC phenomenon. Various PL types and animal models could all produce the ABC phenomenon. However, their extent of accelerated clearance differed. ABC phenomenon is possibly a ubiquitous immune phenomenon in life.

show abstract

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Influence of phospholipid types and animal models on the accelerated blood clearance phenomenon of PEGylated liposomes upon repeated injection

et al. 2014

Drug Delivery

View full text Add to dashboard Cite

show abstract

“…The lipid composition and surface properties play an important role in the binding of biotinylated liposomes to avidin, in vivo blood circulation, and the targeting efficacy of liposomes [29][30][31][32]. In this study, we have used a simple formulation consisting of DPPC as the core lipid, DSPE-PEG2kBiotin as a linker, and NBD-cholesterol as a fluorophore, to investigate the binding efficiency and construction of the new vehicle.…”

Section: Introductionmentioning

confidence: 99%

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Kheirolomoom

Dayton

Lum

et al. 2007

Journal of Controlled Release

206

174

View full text Add to dashboard Cite

A new acoustically-active delivery vehicle was developed by conjugating liposomes and microbubbles, using the high affinity interaction between avidin and biotin. Binding between microbubbles and liposomes each containing 5% DSPE-PEG2kBiotin was highly dependent on avidin concentration and observed above an avidin concentration of 10 nM. With an optimized avidin and liposome concentration, we measured and calculated as high as 1000 to 10,000 liposomes with average diameters of 200 and 100 nm, respectively, attached to each microbubble. Replacing avidin with neutravidin resulted in 3-fold higher binding, approaching the calculated saturation level. Highspeed photography of this new drug delivery vehicle demonstrated that the liposome-bearing microbubbles oscillate in response to an acoustic pulse similar to microbubble contrast agents. Additionally, microbubbles carrying liposomes could be spatially concentrated on a monolayer of PC-3 cells at the focal point of ultrasound beam. As a result of cell-vehicle contact, the liposomes fused with the cells and internalization of NBD-cholesterol occurred shortly after incubation at 37°C, with internalization of NBD-cholesterol substantially enhanced in the acoustic focus.

show abstract

“…It is speculated that high dose liposomes may induce apoptosis of B cells and thus attenuate the phenomenon. liposomes with different chain lengths ( M W 2000 and 5000) were comparable [42] .…”

Section: Lipid Dose Of the First Injectionmentioning

confidence: 90%

“…The blood circulation half-lives (t 1/2 ) of the second dose of PEGylated liposomes were 20, 12, 10 and < 2 h when the first dose was given at 0, 5, 1 and ≤ 0.1 μ mol lipid/kg of body weight, respectively [42] . It is speculated that high dose liposomes may induce apoptosis of B cells and thus attenuate the phenomenon.…”

Section: Lipid Dose Of the First Injectionmentioning

confidence: 99%

Immune responses of therapeutic lipid nanoparticles

Chen¹,

May²,

Li³

2013

Nanotechnology Reviews

View full text Add to dashboard Cite

Nanoparticle-based drug delivery is an emerging technology for targeting therapeutics to the diseased site for enhanced therapy and reduced toxicity. A number of pharmaceutical products that involve nanotechnology have been approved for clinical use, and because of altered pharmacokinetics and biodistribution, their profiles of interaction with host cells and resulting toxicity are different from parent agents. This review focuses on the immune responses induced by therapeutic lipid nanoparticles. These immune responses can provoke toxicity, affect pharmacokinetics of the nanoparticles or induce therapeutic effect. This article begins with a general introduction on immune responses and innate and acquired immunity. Specific examples of therapeutic lipid nanoparticles inducing immune responses in each category are presented with detailed discussions on the mechanisms. Current guidelines for evaluating immune response of nanomedicines are summarized. Finally, perspectives and future directions are provided emphasizing mechanistic studies of immune reactions triggered by nanoparticles.

show abstract

Accelerated blood clearance of PEGylated liposomes following preceding liposome injection: Effects of lipid dose and PEG surface-density and chain length of the first-dose liposomes

Cited by 305 publications

References 34 publications

Influence of phospholipid types and animal models on the accelerated blood clearance phenomenon of PEGylated liposomes upon repeated injection

Influence of phospholipid types and animal models on the accelerated blood clearance phenomenon of PEGylated liposomes upon repeated injection

Acoustically-active microbubbles conjugated to liposomes: Characterization of a proposed drug delivery vehicle

Immune responses of therapeutic lipid nanoparticles

Contact Info

Product

Resources

About