Accelerated Biomethane Potential assay for straw with artificially flocculated sludge and defined ‘synthetic manure’

Scherer, Paul; Arthur, Richard; Antonczyk, Sebastian

doi:10.1016/j.biteb.2021.100787

Cited by 5 publications

(10 citation statements)

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“…A recent study on batch fermentations, supplemented with trace elements and ‘synthetic manure’ in our lab, showed a mean specific methane yield of 287.1 mL STP CH 4 /gVS for the same charge of wheat straw used here, which corresponded to 98% of the theoretical methane yield [ 22 ]. This theoretical methane yield, based on the extended Buswell formula of straw (C 3.71 H 6.04 O 2.79 N 0.044 S 0.005 ,) in Table 1 , was calculated to be 293.4 mL STP CH 4 /gVS.…”

Section: Resultsmentioning

confidence: 89%

“…The ammonium concentration of the fermentation media was controlled mainly by the defined ‘synthetic manure’ ( Table 2 ), it was about 1940 mg/L or 108 mmol L −1 [ 22 ]. The highest ammonia value obtained was 800 mg/L for the thermophilic fermenter F5.…”

Section: Resultsmentioning

confidence: 99%

“…The composition of the ‘synthetic manure’ was based on a long-term chemical analysis of cattle manure from a dairy farm [ 21 ], the cattle were fed with maize and grass. Thereafter, ‘synthetic manure’ was prepared and used for the continuous fermentation experiments, under mesophilic (41 °C) and thermophilic (58 °C) conditions [ 22 ] ( Table 2 ). The alkalinity of the natural cattle manure was found to be in the range of 8000–12,000 mg CaCO 3 eq./L, whereas that of the prepared ‘synthetic manure’ was 10,865 ± 330 mg CaCO 3 eq./L.…”

Section: Methodsmentioning

confidence: 99%

“…Gas volumes were “standardised”, according to IUPAC, with the standard temperature 0 °C and standard pressure 1 bar (STP). For further details, see [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…The presented studies were performed with an automated CSTR system using different supplementation-strategies for nickel and tungsten. Wheat straw was used as mono-substrate with different organic loading rates (OLR) and hydraulic retention times (HRT) in a new, defined medium [ 21 , 22 ], both under mesophilic and thermophilic conditions. Quantitative data of microbial cells, together with qualitative microbiome data about major players of microbial classes and genera, provided a complete assessment of anaerobic straw digestion.…”

Section: Introductionmentioning

confidence: 99%

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Mesophilic and Thermophilic Anaerobic Digestion of Wheat Straw in a CSTR System with ‘Synthetic Manure’: Impact of Nickel and Tungsten on Methane Yields, Cell Count, and Microbiome

et al. 2022

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Lignocellulosic residues, such as straw, are currently considered as candidates for biogas production. Therefore, straw fermentations were performed to quantitatively estimate methane yields and cell counts, as well as to qualitatively determine the microbiome. Six fully automated, continuously stirred biogas reactors were used: three mesophilic (41 °C) and three thermophilic (58 °C). They were fed every 8 h with milled wheat straw suspension in a defined, buffered salt solution, called ‘synthetic manure’. Total reflection X-ray fluorescence spectrometry analyses showed nickel and tungsten deficiency in the straw suspension. Supplementation of nickel and subsequently tungsten, or with an increasing combined dosage of both elements, resulted in a final concentration of approximately 0.1 mg/L active, dissolved tungsten ions, which caused an increase of the specific methane production, up to 63% under mesophilic and 31% under thermophilic conditions. That is the same optimal range for pure cultures of methanogens or bacteria found in literature. A simultaneous decrease of volatile fatty acids occurred. The Ni/W effect occurred with all three organic loading rates, being 4.5, 7.5, and 9.0 g volatile solids per litre and day, with a concomitant hydraulic retention time of 18, 10, or 8 days, respectively. A maximum specific methane production of 0.254 m3 CH4, under standard temperature and pressure per kg volatile solids (almost 90% degradation), was obtained. After the final supplementation of tungsten, the cell counts of methanogens increased by 300%, while the total microbial cell counts increased by only 3–62%. The mesophilic methanogenic microflora was shifted from the acetotrophic Methanosaeta to the hydrogenotrophic Methanoculleus (85%) by tungsten, whereas the H2-CO2-converter, Methanothermobacter, always dominated in the thermophilic fermenters.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Gas volumes were “standardised”, according to IUPAC, with the standard temperature 0 °C and standard pressure 1 bar (STP). For further details, see [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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