Accelerated biogas production from lignocellulosic biomass after pre-treatment with Neocallimastix frontalis

Dollhofer, Veronika; Dandikas, V.; Dorn‐In, Samart; Bauer, Christoph; Lebuhn, Michael; Bauer, Johann

doi:10.1016/j.biortech.2018.05.068

Cited by 30 publications

(14 citation statements)

References 26 publications

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“…It is also reported that selection of substrate specific and as well as effective strain and culture conditions can reduce treatment time and carbohydrate losses [154]. Dollhofer et al [155] reported that biogas production from lignocellulosic biomass can be accelerated after pretreatment with Neocallimastix frontalis, an anaerobic fungus isolated from rumen fluid of a cow and of a chamois.…”

Section: Biological Pretreatmentsmentioning

confidence: 99%

Insight into Pretreatment Methods of Lignocellulosic Biomass to Increase Biogas Yield: Current State, Challenges, and Opportunities

et al. 2019

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Lignocellulosic biomass is recalcitrant due to its heterogeneous structure, which is one of the major limitations for its use as a feedstock for methane production. Although different pretreatment methods are being used, intermediaries formed are known to show adverse effect on microorganisms involved in methane formation. This review, apart from highlighting the efficiency and limitations of the different pretreatment methods from engineering, chemical, and biochemical point of views, will discuss the strategies to increase the carbon recovery in the form of methane by way of amending pretreatments to lower inhibitory effects on microbial groups and by optimizing process conditions.

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Section: Biological Pretreatmentsmentioning

confidence: 99%

Insight into Pretreatment Methods of Lignocellulosic Biomass to Increase Biogas Yield: Current State, Challenges, and Opportunities

et al. 2019

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“…However, these polymers bind with each other and form a strong structure that resists disintegration and hydrolysis during anaerobic digestion (Sawatdeenarunat et al, 2015). To improve the bioconversion of lignocellulosic biomass, previous studies proposed physical (Rusanowska et al, 2018), chemical (Koyama et al, 2017), and biological (Dollhofer et al, 2018) pretreatments. These pretreatment methods enhance methane production from lignocellulosic biomass but consume large amounts of energy and wastewater neutralization and are costly.…”

Section: Introductionmentioning

confidence: 99%

Change of Endoglucanase Activity and Rumen Microbial Community During Biodegradation of Cellulose Using Rumen Microbiota

et al. 2020

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Treatment with rumen microorganisms improves the methane fermentation of undegradable lignocellulosic biomass; however, the role of endoglucanase in lignocellulose digestion remains unclear. This study was conducted to investigate endoglucanases contributing to cellulose degradation during treatment with rumen microorganisms, using carboxymethyl cellulose (CMC) as a substrate. The rate of CMC degradation increased for the first 24 h of treatment. Zymogram analysis revealed that endoglucanases of 52 and 53 kDa exhibited high enzyme activity for the first 12 h, whereas endoglucanases of 42, 50, and 101 kDa exhibited high enzyme activities from 12 to 24 h. This indicates that the activities of these five endoglucanases shifted and contributed to efficient CMC degradation. Metagenomic analysis revealed that the relative abundances of Selenomonas, Eudiplodinium, and Metadinium decreased after 12 h, which was positively correlated with the 52- and 53-kDa endoglucanases. Additionally, the relative abundances of Porphyromonas, Didinium, unclassified Bacteroidetes, Clostridiales family XI, Lachnospiraceae and Sphingobacteriaceae increased for the first 24 h, which was positively correlated with endoglucanases of 42, 50, and 101 kDa. This study suggests that uncharacterized and non-dominant microorganisms produce and/or contribute to activity of 40, 50, 52, 53, and 101 kDa endoglucanases, enhancing CMC degradation during treatment with rumen microorganisms.

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“…Bootten et al [ 112 ] compared the capabilities of rumen fungi ( Caecomyces communis, Piromyces communis and Neocallimastix frontalis ) as mono-culture and co-culturing with Methanobrevibacter smithii in the degradation of lignified Medicago sativa L. Among them, Piromyces communis and Neocallimastix frontalis were the efficient degraders showing 37.9% and 33.2% loss of lignin component in M. sativa , respectively. Recently, Dollhofer et al [ 113 ] investigated the role of two anaerobic fungal strains ( Neocallimastix frontalis ) in hydrolytic pretreatment of hay biomass that was isolated from the rumen fluid of Bos taurus taurus (cow) and Rupicarpa rupicarpa (chamois). The pre-processing of LCB with anaerobic fungi have increased the biogas production together with high volatile fatty acid concentrations.…”

Section: Role Of Microbes and Different Process Strategies Adopted For Biofuel Productionmentioning

confidence: 99%

Unlocking the potential of insect and ruminant host symbionts for recycling of lignocellulosic carbon with a biorefinery approach: a review

et al. 2021

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Uprising fossil fuel depletion and deterioration of ecological reserves supply have led to the search for alternative renewable and sustainable energy sources and chemicals. Although first generation biorefinery is quite successful commercially in generating bulk of biofuels globally, the food versus fuel debate has necessitated the use of non-edible feedstocks, majorly waste biomass, for second generation production of biofuels and chemicals. A diverse class of microbes and enzymes are being exploited for biofuels production for a series of treatment process, however, the conversion efficiency of wide range of lignocellulosic biomass (LCB) and consolidated way of processing remains challenging. There were lot of research efforts in the past decade to scour for potential microbial candidate. In this context, evolution has developed the gut microbiota of several insects and ruminants that are potential LCB degraders host eco-system to overcome its host nutritional constraints, where LCB processed by microbiomes pretends to be a promising candidate. Synergistic microbial symbionts could make a significant contribution towards recycling the renewable carbon from distinctly abundant recalcitrant LCB. Several studies have assessed the bioprospection of innumerable gut symbionts and their lignocellulolytic enzymes for LCB degradation. Though, some reviews exist on molecular characterization of gut microbes, but none of them has enlightened the microbial community design coupled with various LCB valorization which intensifies the microbial diversity in biofuels application. This review provides a deep insight into the significant breakthroughs attained in enrichment strategy of gut microbial community and its molecular characterization techniques which aids in understanding the holistic microbial community dynamics. Special emphasis is placed on gut microbial role in LCB depolymerization strategies to lignocellulolytic enzymes production and its functional metagenomic data mining eventually generating the sugar platform for biofuels and renewable chemicals production.

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Accelerated biogas production from lignocellulosic biomass after pre-treatment with Neocallimastix frontalis

Cited by 30 publications

References 26 publications

Insight into Pretreatment Methods of Lignocellulosic Biomass to Increase Biogas Yield: Current State, Challenges, and Opportunities

Insight into Pretreatment Methods of Lignocellulosic Biomass to Increase Biogas Yield: Current State, Challenges, and Opportunities

Change of Endoglucanase Activity and Rumen Microbial Community During Biodegradation of Cellulose Using Rumen Microbiota

Unlocking the potential of insect and ruminant host symbionts for recycling of lignocellulosic carbon with a biorefinery approach: a review

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