Accelerated Angiogenic Host Tissue Response to Poly(L-Lactide-co-Glycolide) Scaffolds by Vitalization with Osteoblast-like Cells

Tavassol, Frank; Schumann, Paul; Lindhorst, Daniel; Sinikovic, Branko; Voss, André; See, Constantin von; Kampmann, Andreas; Bormann, Kai-Hendrik; Carvalho, Carlos; Mülhaupt, Rolf; Harder, Yves; Laschke, Matthias W.; Menger, Michael D.; Gellrich, Nils‐Claudius; Rücker, Martin

doi:10.1089/ten.tea.2008.0457

Cited by 25 publications

(24 citation statements)

References 57 publications

(66 reference statements)

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“…An improved vascularization of scaffolds is also achieved by seeding them with differentiated tissue-specific cells [11,44] or multipotent stem cells [45,46]. These cells contribute to the vascularization process via different mechanisms.…”

Section: Stimulating Angiogenesis In Tissue Constructsmentioning

confidence: 99%

Vascularization in Tissue Engineering: Angiogenesis versus Inosculation

Laschke

Menger²

2012

Eur Surg Res

171

127

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Background/Purpose: The key challenge in tissue engineering is the establishment of an efficient vascularization for tissue constructs guaranteeing long-term survival and function. Vascularization may be achieved by the stimulation of angiogenesis or the inosculation of preformed microvascular networks within the implants to the host microvasculature. The present review provides an overview of these two concepts applied in tissue engineering. Methods: A literature search was performed in PubMed for publications focusing on vascularization, angiogenesis and inosculation in tissue engineering. Results: Several strategies have been proposed to stimulate the ingrowth of new blood vessels into tissue constructs. These include the modification of the chemical composition and architecture of scaffolds, their bioactivation by incorporation of growth factor delivery systems or by cell seeding as well as the stimulation of stem cell recruitment. However, because angiogenesis is a time-consuming process, all of these approaches cannot prevent ischemic cell death within larger 3-dimensional tissue constructs during the initial phase after implantation. To overcome this problem, in vitro or in situ prevascularization has emerged as a novel concept in tissue engineering. This bears the advantage that preformed microvascular networks within tissue constructs simply have to inosculate with the host microvasculature at the implantation site to get completely blood-perfused within a short period of time. Conclusions: During the last years, considerable progress has been made in the development of promising vascularization strategies in tissue engineering. Particularly the inosculation of preformed microvascular networks has the great potential to markedly improve the survival of tissue constructs after implantation. The optimization of this vascularization strategy may pave the way for a broad clinical use of tissue engineering applications in the future.

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Section: Stimulating Angiogenesis In Tissue Constructsmentioning

confidence: 99%

Vascularization in Tissue Engineering: Angiogenesis versus Inosculation

Laschke

Menger²

2012

Eur Surg Res

171

127

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“…In addition, scaffolds are loaded with antimicrobial peptides, such as cathelicicin LL37 (Steinstraesser et al, 2006), angiogenic growth factors, such as VEGF , or growth factor-containing extracellular matrices, such as Matrigel TM (Laschke et al, 2008a), to stimulate the angiogenic host tissue reaction. Finally, vascularisation of scaffolds can be accelerated by vitalisation with osteoblast-like cells (Schumann et al, 2009;Tavassol et al, 2010) or bone marrow cells (Ichioka et al, 2005;Schumann et al, 2009).…”

Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Laschke

Vollmar²,

Menger³

2011

eCM

144

128

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The implantation of biomaterials into the human body has become an indispensable part of almost all fi elds of modern medicine. Accordingly, there is an increasing need for appropriate approaches, which can be used to evaluate the suitability of different biomaterials for distinct clinical indications. The dorsal skinfold chamber is a sophisticated experimental model, which has been proven to be extremely valuable for the systematic in vivo analysis of the dynamic interaction of small biomaterial implants with the surrounding host tissue in rats, hamsters and mice. By means of intravital fl uorescence microscopy, this chronic model allows for repeated analyses of various cellular, molecular and microvascular mechanisms, which are involved in the early infl ammatory and angiogenic host tissue response to biomaterials during the initial 2-3 weeks after implantation. Therefore, the dorsal skinfold chamber has been broadly used during the last two decades to assess the in vivo performance of prosthetic vascular grafts, metallic implants, surgical meshes, bone substitutes, scaffolds for tissue engineering, as well as for locally or systemically applied drug delivery systems. These studies have contributed to identify basic material properties determining the biocompatibility of the implants and vascular ingrowth into their surface or internal structures. Thus, the dorsal skinfold chamber model does not only provide deep insights into the complex interactions of biomaterials with the surrounding soft tissues of the host but also represents an important tool for the future development of novel biomaterials aiming at an optimisation of their biofunctionality in clinical practice.

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“…21 To verify the identity of the isolated cells as OLC, all isolations were subjected to immunocytochemical detection for collagen I, osteocalcin, and osteonectin. Additionally, alkaline phosphatase was detected via a specific color reaction as described previously in detail 14 (data not shown).…”

Section: Isolation and Cultivation Of Murine Olcmentioning

confidence: 99%

“…Although adequate engraftment of poly(L-lactide-coglycolide) (PLGA) scaffolds has been demonstrated in the past, 12,13 vitalization with OLC has showed rapid death of the seeded cells. 14 This implied that implantation meant hypoxic and physical stress with fatal consequences to the seeded cells before they were reached by a functional microvascular system. 15 Heat shock proteins (HSPs) are found in all organisms and all cell types.…”

Section: Introductionmentioning

confidence: 99%

Prolongated Survival of Osteoblast-Like Cells on Biodegradable Scaffolds by Heat Shock Preconditioning

Tavassol

Kampmann

Lindhorst

et al. 2011

Tissue Engineering Part A

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The implantation of tissue-engineered constructs leads to hypoxic and physical stress to the seeded cells until they were reached by a functional microvascular system. Preconditioning of cells with heat shock induced heat shock proteins, which can support the cells to survive a subsequent episode of stress that would otherwise be lethal. Preconditioning of tissue-engineered constructs resulted in significantly higher number of surviving osteoblast-like cells (OLC). At the 6th and 10th day, angiogenic response was found comparative to poly(L-lactide-co-glycolide) (PLGA) scaffolds vitalized with either unconditioned or preconditioned OLC. However, they were significantly enhanced compared with the nonvitalized collagen-labeled PLGA scaffolds. This study demonstrates that vitalization of PLGA scaffolds with OLC accelerates the angiogenic response induced by the surrounding host tissue. In addition, heat shock preconditioning significantly enhances the survival rate of the OLC that are seeded on these scaffolds. Thus, vitalization of substitutes with adequately pretreated OLC may promise biologically adequate osseous restorations.

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Accelerated Angiogenic Host Tissue Response to Poly(L-Lactide-co-Glycolide) Scaffolds by Vitalization with Osteoblast-like Cells

Abstract: Our study demonstrates that PLGA scaffold vitalization with OLCs accelerates the angiogenic response in the surrounding host tissue. Bone substitutes created by tissue engineering may thus be superior to nonvitalized substitutes although the seeded cells do not survive for long periods.

Cited by 25 publications

References 57 publications

Vascularization in Tissue Engineering: Angiogenesis versus Inosculation

Vascularization in Tissue Engineering: Angiogenesis versus Inosculation

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Prolongated Survival of Osteoblast-Like Cells on Biodegradable Scaffolds by Heat Shock Preconditioning

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