ACC Oxidase and ACC Synthase Expression Profiles after Leaning of Young Radiata (P. radiata D. Don) and Maritime Pine (P. pinaster Ait.) Seedlings

Ramos, Patricio; Valenzuela, Claudio; Provost, Grégoire Le; Plomion, Christophe; Gantz, Carlos; Moya-León, Marı́a Alejandra; Herrera, Raúl

doi:10.1007/s00344-011-9248-2

Cited by 14 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genes involved in ethylene signalling were strongly differentially expressed with ethylene overproducer-like transcripts present in high abundance in contaminated trees, along with others involved in the ethylene biosynthesis pathway, including S-adenosyl-L-methionine (SAM), 1-aminocyclopropane-1-carboxylate (ACC) and ACC oxidase (ACO). Interestingly, when viewed alongside the very high overexpression of FLA12 in the stems of contaminated trees (Additional file 1 : Table S3), a characteristic marker of tension wood tissue in reaction wood producing poplar [ 42 – 44 ] and willow (Brereton, unpublished) trees, an expression profile very similar to tension wood production emerges. Adding to this is a concurrent increase in the abundance of a CesA in stems of contaminated trees as well as the very high abundance of ACO (Additional file 1 : Table S4), thought to result in the secondary xylem tissue localisation (or asymmetry) of ethylene biosynthesis from ubiquitous (non-localised) ACC [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Meta-transcriptomics indicates biotic cross-tolerance in willow trees cultivated on petroleum hydrocarbon contaminated soil

et al. 2015

View full text Add to dashboard Cite

BackgroundHigh concentrations of petroleum hydrocarbon (PHC) pollution can be hazardous to human health and leave soils incapable of supporting agricultural crops. A cheap solution, which can help restore biodiversity and bring land back to productivity, is cultivation of high biomass yielding willow trees. However, the genetic mechanisms which allow these fast-growing trees to tolerate PHCs are as yet unclear.MethodsSalix purpurea ‘Fish Creek’ trees were pot-grown in soil from a former petroleum refinery, either lacking or enriched with C10-C50 PHCs. De novo assembled transcriptomes were compared between tree organs and impartially annotated without a priori constraint to any organism.ResultsOver 45 % of differentially expressed genes originated from foreign organisms, the majority from the two-spotted spidermite, Tetranychus urticae. Over 99 % of T.urticae transcripts were differentially expressed with greater abundance in non-contaminated trees. Plant transcripts involved in the polypropanoid pathway, including phenylalanine ammonia-lyase (PAL), had greater expression in contaminated trees whereas most resistance genes showed higher expression in non-contaminated trees.ConclusionsThe impartial approach to annotation of the de novo transcriptomes, allowing for the possibility for multiple species identification, was essential for interpretation of the crop’s response treatment. The meta-transcriptomic pattern of expression suggests a cross-tolerance mechanism whereby abiotic stress resistance systems provide improved biotic resistance. These findings highlight a valuable but complex biotic and abiotic stress response to real-world, multidimensional contamination which could, in part, help explain why crops such as willow can produce uniquely high biomass yields on challenging marginal land.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0636-9) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Meta-transcriptomics indicates biotic cross-tolerance in willow trees cultivated on petroleum hydrocarbon contaminated soil

et al. 2015

View full text Add to dashboard Cite

show abstract

“…One year-old half-sib Pinus radiata D. Don (radiata pine) seedlings were grown in a local nursery from seeds obtained from an open-pollinated population (half sibs). Seedlings of around 30 cm tall were maintained at 20 °C, and following the protocol established previously 29,91 . Nine inclined seedlings were collected at each sampling time; their stems were cut into different parts along the longitudinal axis into lower and upper sides, pooled, immediately frozen in liquid nitrogen and stored at −80 °C until RNA extraction.…”

Section: Methodsmentioning

confidence: 99%

Induction of PrMADS10 on the lower side of bent pine tree stems: potential role in modifying plant cell wall properties and wood anatomy

Cruz

Méndez

Ramos

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

The molecular mechanisms underlying inclination responses in trees are unclear. In this study, we identified a MADS-box transcription factor differentially expressed early after inclination in the stems of Pinus radiata D. Don. PrMADS10 has a CDS of 582 bp and encodes a group II MADS-box transcription factor. We measured highest accumulation of this transcript on the lower side of inclined pine stems. In an effort to identify putative targets, we stably transformed Arabidopsis thaliana with a 35S::PrMADS10 construct. Transcriptome analysis revealed 1,219 genes differentially-expressed, with 690 and 529 genes up- and down-regulated respectively, when comparing the transgenic and wild-type. Differentially-expressed genes belong to different biological processes, but were enriched in cell wall remodeling and phenylpropanoid metabolic functions. Interestingly, lignin content was 30% higher in transgenic as compared to wild-type plants consistent with observed changes in gene expression. Differentially expressed transcription factors and phenylpropanoid genes were analyzed using STRING. Several MYB and NAC transcription factors showed interactions with genes of the phenylpropanoid pathway. Together, these results implicate PrMADS10 as a regulatory factor, triggering the expression of other transcription factors and genes involved in the synthesis of lignin.

show abstract

“…Pinus radiata D. Don Ethylene biosynthesis-ACC oxidase and ACC synthase differetially expressed. [30] Pinus radiata D. Don Cell division, cellulose biosynthesis, lignin deposition, microtubules. [22] Pinus radiata D. Don Ethylene signaling-Induction of tracheids with compression wood phenotypes in seedlings treated with ethylene biosynthesis precursor.…”

Section: Chamaecyparis Obtusamentioning

confidence: 99%

“…Gene expression analysis has been performed in conifers such as Pinus taeda [ 29 ] and Pinus pinaster [ 17 , 35 ]. In radiata pine, a transcriptional analysis was conducted to evaluate the expression of key genes involved in the ethylene biosynthesis pathway, showing that 1-aminocyclopropane-1-carboxylate synthase (ACS) and 1-aminocyclopropane-1-carboxylate oxidase (ACO) genes are upregulated in inclined stems [ 30 ]. A study of treatments with exogenous ethylene application to radiata pine seedlings showed that the morphological characteristics of CW and lignin deposition on cell walls of the stem of inclined seedlings were accelerated compared to nonethylene-treated seedlings [ 31 ].…”

Section: Hormones and The Gravitropic Response In Gymnospermsmentioning

confidence: 99%

A Talk between Flavonoids and Hormones to Reorient the Growth of Gymnosperms

Morales-Quintana

Ramos

2021

IJMS

Self Cite

View full text Add to dashboard Cite

Plants reorient the growth of affected organs in response to the loss of gravity vector. In trees, this phenomenon has received special attention due to its importance for the forestry industry of conifer species. Sustainable management is a key factor in improving wood quality. It is of paramount importance to understand the molecular and genetic mechanisms underlying wood formation, together with the hormonal and environmental factors that affect wood formation and quality. Hormones are related to the modulation of vertical growth rectification. Many studies have resulted in a model that proposes differential growth in the stem due to unequal auxin and jasmonate allocation. Furthermore, many studies have suggested that in auxin distribution, flavonoids act as molecular controllers. It is well known that flavonoids affect auxin flux, and this is a new area of study to understand the intracellular concentrations and how these compounds can control the gravitropic response. In this review, we focused on different molecular aspects related to the hormonal role in flavonoid homeostasis and what has been done in conifer trees to identify molecular players that could take part during the gravitropic response and reduce low-quality wood formation.

show abstract

ACC Oxidase and ACC Synthase Expression Profiles after Leaning of Young Radiata (P. radiata D. Don) and Maritime Pine (P. pinaster Ait.) Seedlings

Cited by 14 publications

References 47 publications

Meta-transcriptomics indicates biotic cross-tolerance in willow trees cultivated on petroleum hydrocarbon contaminated soil

Meta-transcriptomics indicates biotic cross-tolerance in willow trees cultivated on petroleum hydrocarbon contaminated soil

Induction of PrMADS10 on the lower side of bent pine tree stems: potential role in modifying plant cell wall properties and wood anatomy

A Talk between Flavonoids and Hormones to Reorient the Growth of Gymnosperms

Contact Info

Product

Resources

About