ACC-deaminase and/or nitrogen fixing rhizobacteria and growth of wheat (Triticum Aestivum L.)

Abstract

Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria, which can enhance the growth of the plants, when applied to crops. A pot experiment was conducted to examine the effect of six PGPR isolates on the growth of wheat. Inoculation with rhizobacterial isolates increased the all measured physical, chemical and enzymatic growth parameters compared to control (CK). However, the WAN1 isolate had the highest effect, and significantly (P < 0.05) increased the root length (3.51-fold), shoot length (3.22-fold), seedling fresh (3.41-fold) and dry (3.91-fold) weight, chlorophyll a (3.90-fold), chlorophyll b (3.51-fold), carotenoid contents (7.23-fold), plant macronutrient uptake i.e. N (7.20-fold, 6.71-fold), P (7.41-fold, 5.01-fold), K (5.51-fold, 3.91-fold), Ca (6.40-fold, 5.21-fold) and Mg (5.82-fold, 7.11-fold) in shoot and root, plant micronutrient uptake i.e. Zn (6.40-fold, 9.11-fold), Cu (7.31-fold, 7.02-fold), Fe (6.41-fold, 7.52-fold) and Mn (4.57-fold, 5.21-fold) in shoot and root and plant antioxidant enzymes i.e. glutathione S-transferase (7.51-fold), peroxidase (5.21-fold) and catalase (5.01-fold) respectively. Our results revealed that inoculation of agricultural crops with PGPR is a very useful approach to increase the plant growth. The ACC (1-aminocyclopropane-1-carboxylate) enrichment technique is an efficient approach to select promising PGPR. The PGPR containing dual abilities i.e. both ACC-deaminase and nitrogen fixing ability are more effective than PGPR possessing either ACC-deaminase or nitrogen fixing activity alone for growth promotion of crops.

Efficiency of Rhizobium strains as plant growth promoting rhizobacteria on morpho-physiological properties of Brassica napus L . under salinity stress

“…It seems that a mixture of the above mentioned mechanisms in T5 strain treatment has been the most efficient on plant height, root and shoot dry weight of canola seedlings under salinity stress. Hassan et al (2015) reported similar results.…”

“…In our study, T5 and T4 treatments were the best rhizobacterial isolates due to having combination of plant growth promoting activities. Similarly, Hassan et al (2015) reported that the isolates having both ACC-deaminase activity and nitrogen fixing ability (i.e., WAN1) were more efficient isolates than those of having nitrogen fixing activity or ACC-deaminase activity alone. In our study, only the ACC-deaminase producing strains (T3, T4 and T5) showed significant increase in chlorophyll content compared to the control (Figure 1-C).…”

Impact of inoculant and foliar fertilization on root system parameters of pea (Pisum sativum l.)

“…This partly confirms the findings of Fageria and Moreira (2011), who claim that increasing nutrient supplies in the soil may decrease root length, but increase root weight. In laboratory conditions, Hassan et al (2015) indicated that rhizobacterial isolates increased all measured physical, chemical and enzymatic growth parameters of wheat. Rhizobacteria increased root length, shoot length, dry weight, N uptake by shoots and by roots.…”

Effectiveness of biofertilizer enriched in N by Beijerinckia indica on sugarcane grown on an Ultisol and the interactive effects between biofertilizer and sugarcane filter cake

“…The use of microorganisms is important for sustainable agriculture and plant growth, as they increase the availability and absorption of nutrients, reducing dependence on chemical fertilizers that destabilize agroecosystems (Banerjee et al, 2017;Hassan et al, 2015).…”