Acaulospora longula Spain &amp; N.C. Schenck: A low-cost bioinsumption to optimize phenolics and saponins production in Passiflora alata Curtis

Muniz, Brena Coutinho; Falcão, Eduarda Lins; Monteiro, Rodrigo P.; Santos, Emanuela Lima dos; Bastos-Filho, Carmelo J. A.; Silva, Fábio Sérgio Barbosa da

doi:10.1016/j.indcrop.2021.113498

Cited by 16 publications

(7 citation statements)

References 32 publications

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“…The biomass of some medicinal plants, e.g., Salvia officinalis L. and Melissa officinalis L., increased significantly after inoculation with the single agent Septoglomus viscosum and combined Scutellospora heterogama , Claroideoglomus etunicatum and Acaulospora morrowiae agents ( Tarraf et al., 2017 ; de Assis et al., 2020 ). In addition, increasing evidence has indicated that the contents of many secondary metabolites, such as Passiflora alata Curtis saponin under A. longula ( Muniz et al., 2021 ), O. basilicum essential oil under mixed AMF fertilizer containing Rhizophagus irregularis , Funneliformis mosseae , G. aggregatum ( Yilmaz and Karik, 2022 ) and Thymus vulgaris terpenoids under mixed AMF inoculum of R. intraradices and F. mosseae ( Arpanahi et al., 2020 ), increased in varying degrees by inoculating AMF on the roots. In this study, the P. notoginseng biomass and saponin contents significantly increased, including plant height, total fresh weight, fresh root weight, notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and Rd, and PNSs under mixed AMF of G. intraradices and G. etunicatum ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Dai,

Zhang,

et al. 2024

Front. Plant Sci.

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Panax notoginseng is a highly valued perennial medicinal herb plant in Yunnan Province, China, and the taproots are the main medicinal parts that are rich in active substances of P. notoginseng saponins. The main purpose of this study is to uncover the physiological and molecular mechanism of Panax notoginseng saponin accumulation triggered by methyl jasmonate (MeJA) under arbuscular mycorrhizal fungi (AMF) by determining physiological indices, high-throughput sequencing and correlation analysis. Physiological results showed that the biomass and saponin contents of P. notoginseng, the concentrations of jasmonic acids (JAs) and the key enzyme activities involved in notoginsenoside biosynthesis significantly increased under AMF or MeJA, but the interactive treatment of AMF and MeJA weakened the effect of AMF, suggesting that a high concentration of endogenous JA have inhibitory effect. Transcriptome sequencing results indicated that differential expressed genes (DEGs) involved in notoginsenoside and JA biosynthesis were significantly enriched in response to AMF induction, e.g., upregulated genes of diphosphocytidyl-2-C-methyl-d-erythritol kinases (ISPEs), cytochrome P450 monooxygenases (CYP450s)_and glycosyltransferases (GTs), while treatments AMF-MeJA and salicylhydroxamic acid (SHAM) decreased the abundance of these DEGs. Interestingly, a high correlation presented between any two of saponin contents, key enzyme activities and expression levels of DEGs. Taken together, the inoculation of AMF can improve the growth and saponin accumulation of P. notoginseng by strengthening the activities of key enzymes and the expression levels of encoding genes, in which the JA regulatory pathway is a key link. This study provides references for implementing ecological planting of P. notoginseng, improving saponin accumulation and illustrating the biosynthesis mechanism.

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Section: Discussionmentioning

confidence: 99%

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Dai,

Zhang,

et al. 2024

Front. Plant Sci.

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“…It is generally accepted that the chemical compositions of plants are directly or indirectly related to endophytic microbes and their interactions with host plants [ 48 ]. As the most reliable soil microbiome, AMF was intimately involved in plant physiological and biochemical metabolic processes promoting the production and accumulation of active components in medicinal plants, such as terpenoids, phenols, and alkaloids [ 49 ]. There is evidence that AMF colonization significantly alters the content or composition of secondary metabolites of aromatic plants [ 50 , 51 ], which has been demonstrated in Salvia officinalis L. [ 52 ] and Angelica archagelica L. [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Effect the accumulation of bioactive constituents of a medicinal plant (Salvia Miltiorrhiza Bge.) by arbuscular mycorrhizal fungi community

Wu,

Qin,

Cai

et al. 2023

BMC Plant Biol

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Background Arbuscular mycorrhizal fungi (AMF) form symbiotic relationships with various terrestrial plants and have attracted considerable interest as biofertilizers for improving the quality and yield of medicinal plants. Despite the widespread distribution of AMFs in Salvia miltiorrhiza Bunge's roots, research on the impact of multiple AMFs on biomass and active ingredient accumulations has not been conducted. In this study, the effects of five native AMFs (Glomus formosanum, Septoglomus constrictum, Rhizophagus manihotis, Acaulospora laevis, and Ambispora gerdemannii) and twenty-six communities on the root biomass and active ingredient concentrations of S. miltiorrhiza were assessed using the total factor design method. Results Thirty-one treatment groups formed symbiotic relationships with S. miltiorrhiza based on the pot culture results, and the colonization rate ranged from 54.83% to 89.97%. AMF communities had higher colonization rates and total phenolic acid concentration than single AMF, and communities also appeared to have higher root fresh weight, dry weight, and total phenolic acid concentration than single inoculations. As AMF richness increased, there was a rising trend in root biomass and total tanshinone accumulations (ATTS), while total phenolic acid accumulations (ATP) showed a decreasing trend. This suggests that plant productivity was influenced by the AMF richness, with higher inoculation benefits observed when the communities contained three or four AMFs. Additionally, the affinities of AMF members were also connected to plant productivity. The inoculation effect of closely related AMFs within the same family, such as G. formosanum, S. constrictum, and R. manihotis, consistently yielded lower than that of mono-inoculation when any combinations were applied. The co-inoculation of S. miltiorrhiza with nearby or distant AMFs from two families, such as G. formosanum, R. manihotis, and Ac. laevis or Am. gerdemannii resulted in an increase of ATP and ATTS by more than 50%. AMF communities appear to be more beneficial to the yield of bioactive constituents than the single AMF, but overall community inoculation effects are related to the composition of AMFs and the relationship between members. Conclusion This study reveals that the AMF community has great potential to improve the productivity and the accumulation of bioactive constituents in S. miltiorrhiza, indicating that it is an effective way to achieve sustainable agricultural development through using the AMF community.

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“…For phytochemical evaluations, total phenols were quantified using the method described by Orujei, Shabani, and Sharifi-Tehrani (2013), with tannic acid as the standard curve (y= 7.9482x -0.005; R 2 = 0.9871); the total flavonoids were determined by the method suggested by Araújo et al (2008), of which rutin was used as a standard curve (y= 0.001x -0.0459; R²= 0.9952); total saponins were quantified by the method of Vigo, Narita and Marques (2003), using saponin as standard curve (y= 0.0009x + 0.0045; R 2 = 0.9926) and total proanthocyanidins were evaluated by the methodology of Queiroz, Morais and Nascimento (2002) and catechin was used as standard curve (y= 0.0412 x + 0.0026, R 2 = 0.9926). Details of these analyses have been described by Muniz et al (2021).…”

Section: Experiments Evaluationsmentioning

confidence: 99%

Cultivation protocol using a coir-based substrate modulates the concentration of bioactive compounds and the antioxidant activity of Passiflora alata Curtis seedlings

et al. 2023

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The use of coconut coir dust is a low-cost cultivation practice. Thus, this study aimed to determine the effect of coconut coir dust proportion on foliar secondary metabolite biosynthesis and growth of Passiflora alata Curtis (sweet passion fruit). This Passiflora species possess pharmaceutical relevance and the use of organic-based substrates may promote the production of bioactive compounds in the phytomass. An experiment in a greenhouse was set up with three proportions of coir dust mixed with a commercial substrate for seedlings (CSS) (peat-based) (S1= 1:1:1, CSS: sand: coconut coir dust; S2= 1.5:1.5:1, CSS: sand: coconut coir dust and S3= 1:1, sand: CSS) in seven replicates. After 68 days, growth parameters were measured (height, leaf area, number of leaves, stem diameter, and dry matter), and the harvested leaves were used to prepare ethanolic extracts. The total antioxidant activity and production of phenolics, flavonoids, proanthocyanidins, and saponins were assessed. Unexpectedly, the coir dust supply did not favor the growth and production of secondary metabolites, as the best results were observed in plants grown in S3. Moreover, S3 is efficient in optimizing the growth, metabolite content, and antioxidant capacity of P. alata foliar extracts dispensing coir dust supplementation in the substrate. Therefore, adding coir to CSS is not recommended to enhance the production of secondary metabolites and the growth of P. alata seedlings.

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Acaulospora longula Spain & N.C. Schenck: A low-cost bioinsumption to optimize phenolics and saponins production in Passiflora alata Curtis

Cited by 16 publications

References 32 publications

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Effect the accumulation of bioactive constituents of a medicinal plant (Salvia Miltiorrhiza Bge.) by arbuscular mycorrhizal fungi community

Cultivation protocol using a coir-based substrate modulates the concentration of bioactive compounds and the antioxidant activity of Passiflora alata Curtis seedlings

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