Acaricide resistance and resistance mechanisms in Tetranychus urticae populations from rose greenhouses in the Netherlands

Abstract

Resistance levels to traditional acaricides such as bifenthrin and abamectin were prominent, and might result in control failure under field conditions. Resistance to more recently registered compounds was detected in several populations. Resistance levels were generally unstable in the laboratory without selection pressure. The toxicological, biochemical and genetic data in this study will be essential in devising an efficient resistant management for Dutch rose culture.

A link between host plant adaptation and pesticide resistance in the polyphagous spider mite Tetranychus urticae

“…To relate patterns of response between host adaptation and evolved pesticide resistance, we assessed gene expression patterns between two highly resistant field-collected strains (MR-VP and MAR-AB) and the reference susceptible London strain (37). These field strains, one collected on beans and the second collected on roses (Materials and Methods), are resistant when grown on bean, the host we used for assessing transcriptome variation among the three strains.…”

“…The London reference strain originates from a wild-collected T. urticae population from the Vineland region (Ontario, Canada) and DNA from an inbred line of this strain was used for T. urticae genome sequencing (29). This strain is susceptible to commercially available acaricides (37). The LS-VL laboratory reference strain, originally collected in 2000 near Ghent (Belgium), has been previously described as highly susceptible to acaricides (43).…”

“…However, these studies have not been satisfactory for understanding the scope of acaricide resistance in T. urticae. Under field conditions, multiresistant strains that are resistant to all commercially available acaricides are often encountered, and strikingly these strains also resist compounds with new modes of action that have never been used in the field (37). Here, we have taken advantage of the highquality genome sequence of T. urticae (29,38) to construct an expression microarray that we then used to collect genome-wide expression data over a time course ranging from hours to generations after transfer of mites to a new, challenging host.…”

Evaluation of wild tomato accessions (Solanum spp.) for resistance to two-spotted spider mite (Tetranychus urticae Koch) based on trichome type and acylsugar content

“…However, spider mites have the capacity to quickly develop acaricide resistance (Van Leeuwen et al 2004;Dermauw et al 2012). Many aspects of spider mite biology, including rapid development, high reproductive ability of up to 25 generations per year, and haplo-diploid sex determination facilitate rapid evolution of pesticide resistance (Khajehali et al 2011). Unfortunately, there are no tomato cultivars with resistance to spider mite.…”

Efectos de las condiciones abióticas sobre las interacciones entre tres depredadores de Tetranychus urticae con distintos hábitos alimenticios

“…Es muy importante, debido a su alta fecundidad y corto ciclo de vida, la alternancia entre sustancias activas con distintos modos de acción para evitar el desarrollo de resistencias en pocas generaciones (IRAC 2017). Diversos autores han estudiado diferentes mutaciones y mecanismos de resistencia para T. urticae en distintos cultivos (Lee et al 2003;García-Marí 2005;Van Leeuwen et al 2010, 2015Grbić et al 2011;Khajehali et al 2011;Attia et al 2013;Santamaría et al 2015;Brown et al 2017;Ilias et al 2017). El control químico es una práctica eficaz a corto plazo, lo que a menudo lleva a los productores a utilitzar sustancias que pueden causar serios problemas de eliminación de la fauna útil de T. urticae (Jacas and Garcia-Marí 2001;Kim and Yoo 2002;Castagnoli et al 2005;Duso et al 2008;Sá Argolo et al 2013, 2014.…”