Acaricidal activity of the essential oil from Tetradenia riparia (Lamiaceae) on the cattle tick Rhipicephalus (Boophilus) microplus (Acari; Ixodidae)

Abstract

Tetradenia riparia (Lamiaceae) is a well-known herbal medicine with a variety of useful properties, including its acaricidal effect. This experiment was carried out to study the bioacaricidal activity of T. riparia essential oil (EO) against engorged females of Rhipicephalus (Boophilus) microplus (Acari; Ixodidae). For this purpose, nine serial concentrations (12.50%, 6.25%, 3.75%, 1.80%, 0.90%, 0.45%, 0.22%, 0.11%, and 0.056% w/v) of T. riparia were used for the adult immersion test (AIT). For the larval packet test (LPT), we used 14 serial concentrations (100.00%, 50.00%, 25.00%, 12.50%, 6.25%, 3.65%, 1.82%, 0.91%, 0.45%, 0.228%, 0.114%, 0.057%, 0.028%, and 0.014% w/v). The results for AIT showed 100.00% and 2.05% mortality, 19.00 and 90.20% for the total number of eggs, egg-laying inhibition of 0.00% and 90.20%, hatchability inhibition of 0.00% and 70.23%, and product effectiveness of 100.00% and 2.89%, respectively. The AIT indicated that the LC(50) and LC(99.9), calculated using the Probit test, were for mortality (%) 0.534g/mL (0.436-0.632) and 1.552g/mL (1.183-1.92); for total number of eggs were 0.449g/mL (0.339-0.558) and 1.76g/mL (1.27-2.248); and for hatchability inhibition were 0.114g/mL (0.0-0.31) and 2.462g/mL (1.501-3.422), respectively. Larvae between 14 and 21days old were fasted and placed in each envelope. Bioassays were performed at 27°±1°C, RH⩾80%. Larval mortality was observed 24h after treatment and showed 10.60-100% mortality in the LPT bioassay. The LPT showed that the LC(50) and LC(99.9) were 1.222g/mL (0.655-1.788) and 11.382g/mL (7.84-14.91), respectively. A positive correlation between T. riparia EO concentration and tick control, was observed by the strong acaricidal effects against R. (B.) microplus, and the mortality rate of ticks was dose-dependent. Our results showed that T. riparia is a promising candidate as an acaricide against resistant strains of R. (B.) microplus.

New Natural Diterpene-Type Abietane from Tetradenia riparia Essential Oil with Cytotoxic and Antioxidant Activities

“…Fractions 16 (6.6 mg) and 17 (11.7 mg) were identified by 1 H, 13 C, DEPT, HSQC, HMBC and NOESY, and by comparison with literature data [24]. Thus, fraction 16 was elucidated as a new compound, identified as 9β,13β-epoxy-7-abietene (1) Amorphous white solid; GC retention time 33.78 min.…”

“…Although there is broad report on the isolated compounds of T. riparia essential oil, our group still searches for minor constituents that represent a great challenge for researchers. The essential oil of T. riparia present biological activities that are reported in the literature as antispasmodic [4], larvicidal and insecticidal [1], anti-mycobacterial [11], antimalarial [2], repellent of Anopheles gambiae [12], antimicrobial, antinociceptive [8], and acaricidal against Rhipicephalus (Boophilus) microplus [13].…”

Antileishmanial and immunomodulatory effects of the essential oil from Tetradenia riparia (Hochstetter) Codd

“…Essential oils, including those of the Lamiaceae family, can stimulate cytokines that mediate the protective cellular immune response against intracellular pathogens, other agents, cancer, and autoimmune disorders . The immunomodulatory and antileishmanial effects that were induced by TrEO may be attributable to its complex mixture of compounds.…”

Laboratory and field evaluation of the aphidicidal activity of moso bamboo (Phyllostachys pubescens) leaf extract and identification of the active components

“…Various plant extracts showed aphidicidal activity, including A. triloba , Plocamiaceae , L.honkrauswianus , Kunzeaericifolia , Tetradenia riparia , Commiphora swynertonii and S. miser . The LC 50 values of A. triloba and Tetradenia riparia are 100 and 534 g L −1 , respectively.…”