Acaricidal activity of Annonaceae fractions against Tetranychus tumidus and Tetranychus urticae (Acari: Tetranychidae) and the metabolite profile of Duguetia lanceolata (Annonaceae) using GC-MS

Abstract

Species of the Tetranychus genus feed on plant tissues, which reduces the rate of photosynthesis and can lead to the death of plant tissues. As a result, considerable production losses are caused by these arthropods. Thus, in order to aid in the development of new products for the control of Tetranychus tumidus Banks and Tetranychus urticae Koch (Acari: Tetranychidae), the initial objective of this study was to select Annonaceae derived fractions that were soluble in dichloromethane and have acaricidal activity. Then, an exploratory analysis of the metabolite profile of the most successful fraction was performed by gas chromatography-mass spectrometry (GC-MS). Among the dichloromethane soluble fractions derived from Annona cacans Warm., Annona coriacea Mart., Annona neolaurifolia H. Rainer, Annona sylvatica A.St.-Hil., Duguetia lanceolata A.St.-Hil., Guatteria australis A.St.-Hil., Xylopia brasiliensis Spreng., Xylopia emarginata Mart. and Xylopia sericea A.St.-Hil., only the fraction from D. lanceolata stem bark reduced the survival of T. tumidus females. However, ovicidal activity was not detected when D. lanceolata stem bark was evaluated against T. tumidus eggs. Further, we studied the effect of dichloromethane soluble fractions from D. lanceolata leaves, berry fruits and stem bark on T. urticae, and the stem bark was found to be the most active fraction against T. urticae. The metabolite profile analysis of D. lanceolata stem bark by GC-MS, suggested that the main constituents were 2,4,5-trimethoxystyrene and trans-asarone. Key words: Botanic acaricide, secondary metabolites, 1,2,4-trimethoxy-5-vinylbenzene, 1,2,4-trimethoxy-5-[(1E)-1-propen-1-yl]benzene ResumoAs espécies do gênero Tetranychus alimentam-se de tecidos vegetais acarretando redução na capacidade fotossintética da planta, que pode resultar em morte dos tecidos vegetais, como consequência são

Dereplication of Aporphine Alkaloids by UHPLC-HR-ESI-MS/MS and NMR from Duguetia lanceolata St.-Hil (Annonaceae) and Antiparasitic Activity Evaluation

“…8 Until now, there are few chemical reports about D. lanceolata in literature, and some studies describe identification of trans-asarone, 2,4,5-trymethoxystirene, stigmasterol, sitosterol and campesterol from its leaves. 9,10 Furthermore, the chemical composition of its essential oil from bark and its biological activities has also been reported. 11,12 Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS) has been widely used as an important tool for dereplication of natural product extracts, including alkaloidal fractions.…”

Identification of antifungal constituents of essential oils extracted from Boesenbergia pulcherrima against Fusarium wilt (Fusarium oxysporum)

“…It has pharmacological effects on central nervous system disorders, such as dementia [18][19][20]. 1,2,4-Trimethoxy-5-vinylbenzene has been reported in Duguetia lanceolata and has been reported to be active against spotted mites [21].…”

Bioactivity of the organic extracts of Annona vepretorum on Tetranychus urticae (Acari: Tetranychidae)

“…The Annonaceae stands out from the several botanical families that release metabolites that are toxic to the phytophagous mites, and their derivatives have been widely tested as an alternative to control mites (Madhumitha et al, 2012;Ribeiro et al, 2014;Alves et al, 2015). Brazil accounts for 29 genera of this family, which comprises nearly 392 species (Maas et al, 2015), several of which, particularly those of the genus Annona, have been investigated for pest control (Krinski et al, 2014;Ribeiro et al, 2016).…”