Acanthopanax senticosus extracts have a protective effect on Drosophila gut immunity

Li, Wenjia; Luo, Qiong; Jin, Li

doi:10.1016/j.jep.2012.12.040

Cited by 20 publications

(25 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditional, medicinal plants are globally used and have rapidly grown in economic importance. Intrinsically active compounds are well-known for their anti-oxidant, anti-tumor, anti-viral and anti-inflammatory activities, and for improving immunity in general (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage

Zhou

Liu

Chen

et al. 2016

Experimental and Therapeutic Medicine

Self Cite

View full text Add to dashboard Cite

Traditional medicinal plants are widely used as immunomodulatory medicines that help improve health. A total of 50 different plants used for the treatment of toxicity were screened for their protective effects. Flies were fed a standard cornmeal-yeast medium (control group) or the standard medium containing medicinal plant extracts (experimental groups). Assessment of the survival rate was performed by feeding flies with toxic compounds. Gut epithelial cells were analyzed for cell proliferation and death by green fluorescent protein antibodies and 7-aminoactinomycin D staining under the microscope. The expression of antimicrobial peptides (AMPs) was evaluated by the quantitative polymerase chain reaction and the results revealed that after feeding the flies with toxic compounds, aqueous extracts from (Franch.) Nannf (), (Decne.) C.B.Clarke (), Beauv.var. (Nees) C.E. Hubb. ( var. ) and Sied. Et Zucc. () increased the fly survival rate, reduced epithelial cell death and improved gut morphology. In addition, extracts induced the antimicrobial peptide levels (Dpt and Mtk) following treatment with sodium dodecyl sulfate (SDS). However, these extracts were not observed to increase SDS-induced cell proliferation. These results indicate that there are strong protective effects in extracts of ,, var. and on intestinal cells among 50 medicinal plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage

Zhou

Liu

Chen

et al. 2016

Experimental and Therapeutic Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…An aqueous extracts of R . crenulata root were obtained as previously described (Li et al ., ). Flies were fed a standard cornmeal–yeast medium containing 2.5% (w/v) of R .…”

Section: Methodsmentioning

confidence: 97%

“…The pellets were resuspended in feeding medium. The spores of Beauveria bassiana were harvested as previously described (Li et al ., ). The 3‐ to 5‐day‐old adult flies (15 female and 15 male) were starved for 2 h before being transferred to a vial containing five layers of filter paper hydrated with 5% sucrose (w/v) with microorganisms and toxic compound (Ha et al ., ), containing S .…”

Section: Methodsmentioning

confidence: 97%

“…Imaging and 7‐amino‐actinomycin D (7‐AAD) staining were performed as previously described (Li et al ., ). In brief, the dissected guts were stained with 7‐AAD [5 µg/mL in phosphate buffered saline (PBS); Invitrogen, Carlsbad, CA, USA] for 30 min at room temperature, fixed for 30 min in PBS with 4% paraformaldehyde, and then stained with 4′,6‐diamidino‐2‐phenylindole for 10 min.…”

Section: Methodsmentioning

confidence: 97%

See 1 more Smart Citation

The Protective Effects of Rhodiola crenulata Extracts on Drosophila melanogaster Gut Immunity Induced by Bacteria and SDS Toxicity

Zhu

Guan

Wang

et al. 2014

Phytotherapy Research

Self Cite

View full text Add to dashboard Cite

The aim of this study was to observe the effect of the Rhodiola crenulata extracts on gut immunity of Drosophila melanogaster. Wild-type flies fed standard cornmeal-yeast medium were used as controls. Experimental groups were supplemented with 2.5% R. crenulata aqueous extracts in standard medium. Survival rate was determined by feeding pathogenic microorganisms and toxic compounds. The levels of reactive oxygen species and dead cells were detected by dihydroethidium and 7-amino-actinomycin D staining, respectively. The expression of antimicrobial peptides was evaluated by quantitative polymerase chain reaction, and morphological change of the intestine was imaged by an Axioskop 2 plus microscope. The results demonstrate that R. crenulata increased the survival rates of adult flies and expression of antimicrobial peptide genes after pathogen or toxic compound ingestion. Moreover, decreased levels of reactive oxygen species and epithelial cell death were associated with results in improved intestinal morphology. The pharmacological action of R. crenulata from Tibet was greater than that from Sichuan. These results indicate that the R. crenulata extracts from Tibet had better pharmacological effect on D. melanogaster gut immunity after ingestion of pathogens and toxic compounds. These results may provide the pharmacological basis for prevention of inflammatory diseases of the intestine.

show abstract

“…Aqueous CI extract was obtained as previously described (11). Chopped capitula (20 g) were soaked overnight in deionized water (200 ml; yield, ~5–14%) at room temperature and then heated until boiling for 3 h. The extraction process was repeated twice and the filtrate was collected and concentrated to 100 ml.…”

Section: Methodsmentioning

confidence: 99%

Flos�Chrysanthemi�Indici extract improves a high‑sucrose diet‑induced metabolic disorder in Drosophila

Bai

Shao

et al. 2018

Exp Ther Med

Self Cite

View full text Add to dashboard Cite

Flos Chrysanthemi Indici (CI) is a traditional medicinal plant used in the treatment of inflammatory diseases. However, the pharmacological role of CI in metabolic diseases, especially in diseases induced by insulin metabolism disorders, remains poorly understood. In the present study, Drosophila melanogaster (Drosophila) were fed with high-sugar diet (HSD) to induce a model similar to Type 2 diabetes (T2D) in order to determine whether CI extracts improve the metabolic disorder. It was demonstrated that the CI extracts could improve growth rate, body size, lifespan, reproductive capacity and fat storage, and CI especially improved the fat metabolism and cell size in S6k and Akt1 mutant flies. In conclusion, the present study provides novel evidence that CI may be an effective drug for the treatment of T2D.

show abstract

Acanthopanax senticosus extracts have a protective effect on Drosophila gut immunity

Cited by 20 publications

References 26 publications

Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage

Identification of the protective effects of traditional medicinal plants against SDS-induced Drosophila gut damage

The Protective Effects of Rhodiola crenulata Extracts on Drosophila melanogaster Gut Immunity Induced by Bacteria and SDS Toxicity

Flos�Chrysanthemi�Indici extract improves a high‑sucrose diet‑induced metabolic disorder in Drosophila

Contact Info

Product

Resources

About